Medicolegal identification of seminal fluid by the radioimmunoassay of prostatic acid phosphatase

K. Okamura, Yuji Yamamoto, H. Ishizu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

For the purpose of applying a radioimmunoassay (RIA) of prostatic acid phosphatase (PAP) for the medicolegal identification of seminal fluids and seminal stains, the usefulness of this method has been investigated for a comparison with the conventional method of an enzyme assay (EA). The detection limit of the PAP level was found to be about 0.5 ng/ml by the RIA method, while that of the PAP activity was about 0.2 King-Armstrong units (K.A.U.) by the EA method. The mean concentration and activity of PAP in the seminal fluid were 1,450,690 ng/ml and 563,290 K.A.U. respectively, these values being markedly higher than those of other human body fluids and secreta. The PAP contents and activities in the seminal fluids from normal, oligospermic and azoospermic human subjects were not significantly (p

Original languageEnglish
Pages (from-to)385-398
Number of pages14
JournalJapanese Journal of Legal Medicine
Volume41
Issue number5
Publication statusPublished - 1987
Externally publishedYes

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Radioimmunoassay
Enzyme Assays
Body Fluids
Human Body
Limit of Detection
Coloring Agents
prostatic acid phosphatase

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Medicolegal identification of seminal fluid by the radioimmunoassay of prostatic acid phosphatase. / Okamura, K.; Yamamoto, Yuji; Ishizu, H.

In: Japanese Journal of Legal Medicine, Vol. 41, No. 5, 1987, p. 385-398.

Research output: Contribution to journalArticle

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