Medical protein separation system using high gradient magnetic separation by superconducting magnet

Y. Kamioka, K. Agatsuma, K. Kajikawa, Hiroshi Ueda, M. Furuse, S. Fuchino, T. Iitsuka, S. Nakamura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

A high gradient magnetic separation system for medical protein using affinity magnetic nano-beads has been developed. Medical protein such as monoclonal antibody or immunoglobulin is an important substance as a medicine for cancer etc. However; the separation system of these medical protein has very low separation rate and the cost of product is extremely high. The developed system shows very high separation efficiency and can achieve low cost by large production rate compared to the system now using in this field. The system consists of a 3T superconducting magnet cooled by a cryo-cooler, a filter made of fine magnetic metal wires of about 30μm diameter and a demagnetization circuit and a liquid circulation pump for solvent containing medical protein. Affinity magnetic nano-beads is covered with the medical protein after agitation of solvent containing the protein and nano-beads, then the solvent flows through the system and the beads are trapped in the filters by high gradient magnetic field. The beads are released and flow out of the system by the AC demagnetization of the filters using LC resonance circuits after discharge of the magnet. The test results shows 97.8% of the magnetic nano-beads in pure water were captured and 94.1% of total beads were collected.

Original languageEnglish
Title of host publicationAdvances in Cryogenic Engineering - Transactions of the Cryogenic Engineering Conference - CEC, Volume 59
EditorsSusan Breon, Jennifer Marquardt, Thomas Peterson, Michael DiPirro, John Pfotenhauer, Jonathan Demko, James Fesmire, Arkadiy Klebaner, Sidney Yuan, Al Zeller, Peter Kittel, Gregory Nellis, Mark Zagarola
PublisherAmerican Institute of Physics Inc.
Pages1129-1134
Number of pages6
ISBN (Electronic)9780735412019
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes
Event2013 Joint Cryogenic Engineering and International Cryogenic Materials Conferences, CEC/ICMC 2013 - Anchorage, United States
Duration: Jun 17 2013Jun 21 2013

Publication series

NameAIP Conference Proceedings
Volume1573
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2013 Joint Cryogenic Engineering and International Cryogenic Materials Conferences, CEC/ICMC 2013
CountryUnited States
CityAnchorage
Period6/17/136/21/13

Keywords

  • Affinity magnetic nanobeads
  • Cryo-cooler cooled superconducting magnet
  • High gradient magnetic separation
  • Immunoglobulin
  • Medical protein

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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  • Cite this

    Kamioka, Y., Agatsuma, K., Kajikawa, K., Ueda, H., Furuse, M., Fuchino, S., Iitsuka, T., & Nakamura, S. (2014). Medical protein separation system using high gradient magnetic separation by superconducting magnet. In S. Breon, J. Marquardt, T. Peterson, M. DiPirro, J. Pfotenhauer, J. Demko, J. Fesmire, A. Klebaner, S. Yuan, A. Zeller, P. Kittel, G. Nellis, & M. Zagarola (Eds.), Advances in Cryogenic Engineering - Transactions of the Cryogenic Engineering Conference - CEC, Volume 59 (pp. 1129-1134). (AIP Conference Proceedings; Vol. 1573). American Institute of Physics Inc.. https://doi.org/10.1063/1.4860832