Medaka (Oryzias latipes) as a model for hypoosmoregulation of euryhaline fishes

Tatsuya Sakamoto, Tomohiro Kozaka, Akiyoshi Takahashi, Hiroshi Kawauchi, Masaaki Ando

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We examined the hypoosmoregulatory ability of a model fish, medaka (Oryzias latipes), in relation to the gill-chloride cells or mitochondrion-rich (MR) cells, and to cortisol. When the medaka were transferred from freshwater (FW) to 30% seawater (SW), muscle water content decreased by 8% after 2 h and normalized within 1 week. Size and density of MR cells in the gill filament increased after 1-2 weeks in SW. Immersion of medaka in FW containing cortisol (10 μg/ml) for 1-2 weeks also doubled the number of MR cells, and abolished the decrease in muscle water content after SW transfer. In SW-adapted medaka, prolactin (PRL; 10 μg/g body weight) injection reduced muscle water content. However, when cortisol (50 μg/g) was injected simultaneously with PRL, cortisol abolished the decrease in muscle water. We concluded that cortisol plays an important role in the SW adaptation of the medaka, whereas PRL may be involved in ion uptake. Medaka seems to be a good model fish useful for the study of osmoregulatory mechanisms in general.

Original languageEnglish
Pages (from-to)347-354
Number of pages8
JournalAquaculture
Volume193
Issue number3-4
DOIs
Publication statusPublished - Feb 15 2001
Externally publishedYes

Fingerprint

euryhaline species
Oryzias latipes
cortisol
seawater
muscle
mitochondrion
fish
muscles
mitochondria
water content
gills
cells
ion transport
prolactin
chlorides
chloride
injection
body weight
ion
water

Keywords

  • Chloride cell
  • Cortisol
  • Medaka
  • Oryzias latipes
  • Osmoregulation
  • Prolactin

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Medaka (Oryzias latipes) as a model for hypoosmoregulation of euryhaline fishes. / Sakamoto, Tatsuya; Kozaka, Tomohiro; Takahashi, Akiyoshi; Kawauchi, Hiroshi; Ando, Masaaki.

In: Aquaculture, Vol. 193, No. 3-4, 15.02.2001, p. 347-354.

Research output: Contribution to journalArticle

Sakamoto, Tatsuya ; Kozaka, Tomohiro ; Takahashi, Akiyoshi ; Kawauchi, Hiroshi ; Ando, Masaaki. / Medaka (Oryzias latipes) as a model for hypoosmoregulation of euryhaline fishes. In: Aquaculture. 2001 ; Vol. 193, No. 3-4. pp. 347-354.
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