Mechanisms of the selenium tolerance of the Arabidopsis thaliana knockout mutant of sulfate transporter SULTR1;2

Misao Ohno, Misugi Uraji, Yasuaki Shimoishi, Izumi Mori, Yoshimasa Nakamura, Yoshiyuki Murata

Research output: Contribution to journalArticle

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Abstract

We investigated the mechanism of selenium (Se) tolerance using an Arabidopsis thaliana knockout mutant of a sulfate transporter, sultr1;2. Se stress inhibited plant growth, decreased chlorophyll contents, and increased protein oxidation and lipid peroxidation in the wild type, whereas the sultr1;2 mutation mitigated damage of these forms, indicating that sultr1;2 is more tolerant of Se than the wild type is. The accumulation of symplastic Se was suppressed in sultr1;2 as compared to the wild type, and the chemical speciation of Se in the mutant was different from that in the wild type. Regardless of Se stress, the activities of ascorbate peroxidase, catalase, and peroxidase in the mutant were higher than in the wild type, while the activity of superoxide dismutase in the mutant was the same as in the wild type. These results suggest that the sultr1;2 mutation confers Se tolerance on Arabidopsis by decreasing symplastic Se and maintaining antioxidant enzyme activities.

Original languageEnglish
Pages (from-to)993-998
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number5
DOIs
Publication statusPublished - 2012

Fingerprint

Selenium
Arabidopsis
Sulfates
Chemical speciation
Ascorbate Peroxidases
Mutation
Enzyme activity
Chlorophyll
Catalase
Lipid Peroxidation
Peroxidase
Superoxide Dismutase
Antioxidants
Lipids
Oxidation
Enzymes
Growth

Keywords

  • Antioxidant activity
  • Arabidopsis thaliana
  • Selenium
  • Sultr1;2
  • Uptake

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Mechanisms of the selenium tolerance of the Arabidopsis thaliana knockout mutant of sulfate transporter SULTR1;2. / Ohno, Misao; Uraji, Misugi; Shimoishi, Yasuaki; Mori, Izumi; Nakamura, Yoshimasa; Murata, Yoshiyuki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 5, 2012, p. 993-998.

Research output: Contribution to journalArticle

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