Mechanisms of the genotoxic effects associated with 5-formyluracil: effect of exogenous 5'-formyl-2'-deoxyuridine.

H. Ide, Hiroaki Terato, A. Masaoka, N. Nagasawa, Y. Ohyama

Research output: Contribution to journalArticle

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Abstract

E. coli HB101 harboring plasmid pUC19 was grown in the presence of 5-formyl-2'-deoxyuridine (fdU) to evaluate the genotoxic and cytotoxic potentials associated with this DNA lesion. Cell growth was inhibited by fdU in a concentration-dependent manner, but increased mutation was not observed in the lacZ(alpha) gene of pUC19. The lack of the mutagenic effect was attributed to poor utilization of fdU as a substrate by thymidine kinase, which converts exogenous thymidine analogs to the corresponding 5'-monophosphates in the salvage pathway.

Original languageEnglish
Pages (from-to)165-166
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - Jan 1 1997
Externally publishedYes

Fingerprint

Deoxyuridine
Lac Operon
Thymidine Kinase
Thymidine
Plasmids
Escherichia coli
Mutation
DNA
Growth
5-formyluracil
5-formyl-2'-deoxyuridine

Cite this

Mechanisms of the genotoxic effects associated with 5-formyluracil : effect of exogenous 5'-formyl-2'-deoxyuridine. / Ide, H.; Terato, Hiroaki; Masaoka, A.; Nagasawa, N.; Ohyama, Y.

In: Nucleic acids symposium series, No. 37, 01.01.1997, p. 165-166.

Research output: Contribution to journalArticle

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