Mechanisms of the genotoxic effects associated with 5-formyluracil: effect of exogenous 5'-formyl-2'-deoxyuridine.

H. Ide, H. Terato, A. Masaoka, N. Nagasawa, Y. Ohyama

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Abstract

E. coli HB101 harboring plasmid pUC19 was grown in the presence of 5-formyl-2'-deoxyuridine (fdU) to evaluate the genotoxic and cytotoxic potentials associated with this DNA lesion. Cell growth was inhibited by fdU in a concentration-dependent manner, but increased mutation was not observed in the lacZ(alpha) gene of pUC19. The lack of the mutagenic effect was attributed to poor utilization of fdU as a substrate by thymidine kinase, which converts exogenous thymidine analogs to the corresponding 5'-monophosphates in the salvage pathway.

Original languageEnglish
Pages (from-to)165-166
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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