Mechanisms of inhibitory effects of polycyclic aromatic hydrocarbons in photosynthetic primary processes in pea leaves and thylakoid preparations

V. D. Kreslavski, M. Brestic, S. K. Zharmukhamedov, V. Yu Lyubimov, A. V. Lankin, A. Jajoo, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Inhibitory effects of polycyclic aromatic hydrocarbons (PAHs) on plants were studied in pea leaves in order to elucidate the mechanisms of action of PAHs such as naphthalene (Naph) and phenanthrene (Phen) on activity of photosystem II (PSII). The changes in different Chl fluorescence parameters were calculated on the basis of Chl fluorescence induction curves. H2O2 content was measured in leaf homogenates with the luminol-dependent chemiluminescence method. We demonstrated that following PAH treatment, total energy dissipation (DI0/ABS) and amount of QB-non-reducing complexes of PSII significantly increased. Non-photochemical quenching (NPQ) also increased, when weak oxidative stress after PAH application developed. In leaves, a two-step increase in H2O2 was found with time of incubation in the presence of PAHs, which may be associated with damage to the lipid bilayer of the plasma membrane and then violation of lipid bilayer membranes of cell organelles. A hypothesis for the mode of action of PAHs is provided that involves the role of ROS, membrane permeability and associated functional changes in PSII.

Original languageEnglish
Pages (from-to)683-688
Number of pages6
JournalPlant Biology
Volume19
Issue number5
DOIs
Publication statusPublished - Sep 1 2017
Externally publishedYes

Keywords

  • Naphthalene
  • phenanthrene
  • photosynthetic apparatus
  • photosystem II
  • polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Fingerprint Dive into the research topics of 'Mechanisms of inhibitory effects of polycyclic aromatic hydrocarbons in photosynthetic primary processes in pea leaves and thylakoid preparations'. Together they form a unique fingerprint.

  • Cite this

    Kreslavski, V. D., Brestic, M., Zharmukhamedov, S. K., Lyubimov, V. Y., Lankin, A. V., Jajoo, A., & Allakhverdiev, S. (2017). Mechanisms of inhibitory effects of polycyclic aromatic hydrocarbons in photosynthetic primary processes in pea leaves and thylakoid preparations. Plant Biology, 19(5), 683-688. https://doi.org/10.1111/plb.12598