Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium

Ezat Asgarani, Hirofumi Funamizu, Takeshi Saito, Hiroaki Terato, Yoshihiko Ohyama, Osamu Yamamoto, Ide Hiroshi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalMicrobiological Research
Volume154
Issue number2
DOIs
Publication statusPublished - Sep 1999
Externally publishedYes

Keywords

  • Carotenoid
  • DNA damage
  • Halophilic bacteria
  • Intracellular salt
  • Resistance

ASJC Scopus subject areas

  • Microbiology

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