Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium

Ezat Asgarani, Hirofumi Funamizu, Takeshi Saito, Hiroaki Terato, Yoshihiko Ohyama, Osamu Yamamoto, Ide Hiroshi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalMicrobiological Research
Volume154
Issue number2
DOIs
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Halobacterium
Ionizing Radiation
Bacteria
DNA
Pyrimidine Dimers
Carotenoids
Single-Stranded DNA Breaks
DNA Damage
Plasmids
bacterioruberin

Keywords

  • Carotenoid
  • DNA damage
  • Halophilic bacteria
  • Intracellular salt
  • Resistance

ASJC Scopus subject areas

  • Microbiology

Cite this

Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium. / Asgarani, Ezat; Funamizu, Hirofumi; Saito, Takeshi; Terato, Hiroaki; Ohyama, Yoshihiko; Yamamoto, Osamu; Hiroshi, Ide.

In: Microbiological Research, Vol. 154, No. 2, 01.01.1999, p. 185-190.

Research output: Contribution to journalArticle

Asgarani, E, Funamizu, H, Saito, T, Terato, H, Ohyama, Y, Yamamoto, O & Hiroshi, I 1999, 'Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium', Microbiological Research, vol. 154, no. 2, pp. 185-190. https://doi.org/10.1016/S0944-5013(99)80013-5
Asgarani, Ezat ; Funamizu, Hirofumi ; Saito, Takeshi ; Terato, Hiroaki ; Ohyama, Yoshihiko ; Yamamoto, Osamu ; Hiroshi, Ide. / Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium. In: Microbiological Research. 1999 ; Vol. 154, No. 2. pp. 185-190.
@article{a59324e4fea744858330ed90dd72980d,
title = "Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium",
abstract = "Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.",
keywords = "Carotenoid, DNA damage, Halophilic bacteria, Intracellular salt, Resistance",
author = "Ezat Asgarani and Hirofumi Funamizu and Takeshi Saito and Hiroaki Terato and Yoshihiko Ohyama and Osamu Yamamoto and Ide Hiroshi",
year = "1999",
month = "1",
day = "1",
doi = "10.1016/S0944-5013(99)80013-5",
language = "English",
volume = "154",
pages = "185--190",
journal = "Microbiological Research",
issn = "0944-5013",
publisher = "Urban und Fischer Verlag Jena",
number = "2",

}

TY - JOUR

T1 - Mechanisms of DNA protection in Halobacterium salinarium, an extremely halophilic bacterium

AU - Asgarani, Ezat

AU - Funamizu, Hirofumi

AU - Saito, Takeshi

AU - Terato, Hiroaki

AU - Ohyama, Yoshihiko

AU - Yamamoto, Osamu

AU - Hiroshi, Ide

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.

AB - Halobacterium salinarium exhibits resistance to ultraviolet (UV) and ionizing radiation. This organism contains carotenoids and also accumulates highly concentrated KCl in the cell. In the present study, DNA lesions generated by UV and ionizing radiation were measured in vitro in the presence and absence of bacterioruberin, the major carotenoid of H. salinarium, and KCl to elucidate their influences on DNA damage production. When plasmid DNA (pDEL19) was UV-irradiated, formation of cyclobutane pyrimidine dimers (CPD) was slightly suppressed by 0.1 mM bacterioruberin. The same concentration of bacterioruberin suppressed the formation of DNA single strand breaks (SSB) by ionizing radiation more efficiently. The formation of CPD by UV and SSB by ionizing radiation was also repressed by 2M KCl but protection against ionizing radiation was extremely efficient.

KW - Carotenoid

KW - DNA damage

KW - Halophilic bacteria

KW - Intracellular salt

KW - Resistance

UR - http://www.scopus.com/inward/record.url?scp=0032868990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032868990&partnerID=8YFLogxK

U2 - 10.1016/S0944-5013(99)80013-5

DO - 10.1016/S0944-5013(99)80013-5

M3 - Article

AN - SCOPUS:0032868990

VL - 154

SP - 185

EP - 190

JO - Microbiological Research

JF - Microbiological Research

SN - 0944-5013

IS - 2

ER -