Mechanism of Tetravalent Manganese Reduction with Elemental Sulfur by Thiobacillus ferrooxidans

Tsuyoshi Sugio, Yoshihiko Tsujita, Kouichi Hirayama, Kenji Inagaki, Tatsuo Tano

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9 Citations (Scopus)

Abstract

Washed intact cells of iron-grown T. ferrooxidans API9-3 could reduce manganese dioxide (MnO2) enzymatically with elemental sulfur (S°) and the mechanism of Mn4+ reduction was studied. The optimum pH for Mn4+ reduction was 2.5. The amount of Mn4+ reduced by the strain was proportional to the amount of S° added to the reaction mixture. Anenzyme that directly catalyzes the reduction of Mn4+ to Mn2+ with S° was not found in the cell-free extract of this strain. T. ferrooxidans API9-3 was found to possess sulfur: ferric ion oxidoreductase (SFORase), which catalyzes the reduction of Fe3+ with S° to give Fe2+ and sulfite. The reduction of Mn4+ was inhibited by a specific inhibitor of SFORase or diamide, strongly suggesting that SFORase is involved in the Mn4+ reduction in this strain. The involvement of SFORase in Mn4+ reduction was further supported by the following results. Both the amounts of Fe2+ and sulflte produced by SFORase were markedly reduced by MnO2. The amount of Mn4+ reduced increased 5.4-fold with 1 mm ferric ion and 10-fold with 2.5 mm cyanide or an inhibitor of iron oxidase, respectively. The sulfite and Fe2+ were chemically oxidized by MnO2 instantly. From the results, it is concluded that the Mn4+ reduction with S° in this strain occurs through two steps. In the first step S° is enzymatically oxidized by SFORase to give Fe2+ and sulfite, and in the second step these two reduced compounds are chemically reduced by MnO2 to give Mn2+.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalAgricultural and Biological Chemistry
Volume52
Issue number1
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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