Mechanism of gene expression of arabidopsis glutathione S-transferase, AtGST1, and AtGST11 in response to aluminum stress

Bunichi Ezaki, Masakatsu Suzuki, Hirotoshi Motoda, Masako Kawamura, Susumu Nakashima, Hideaki Matsumoto

Research output: Contribution to journalArticle

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Abstract

The gene expression of two Al-induced Arabidopsis glutathione S-transferase genes, AtGST1 and AtGST11, was analyzed to investigate the mechanism underlying the response to Al stress. An approximately 1-kb DNA fragment of the 5′-upstream region of each gene was fused to a β-glucuronidase (GUS) reporter gene (pAtGST1::GUS and pAtGST11::GUS) and introduced into Arabidopsis ecotype Landsberg erecta. The constructed transgenic lines showed a time-dependent gene expression to a different degree in the root and/or leaf by Al stress. The pAtGST1::GUS gene was induced after a short Al treatment (maximum expression after a 2-h exposure), while the pAtGST11::GUS gene was induced by a longer Al treatment (approximately 8 h for maximum expression). Since the gene expression was observed in the leaf when only the root was exposed to Al stress, a signaling system between the root and shoot was suggested in Al stress. A GUS staining experiment using an adult transgenic line carrying the pAtGST11::GUS gene supported this suggestion. Furthermore, Al treatment simultaneously with various Ca depleted conditions in root region enhanced the gene expression of the pAtGST11::GUS in the shoot region. This result suggested that the degree of Al toxicity in the root reflects the gene response of pAtGST11::GUS in the shoot via the deduced signaling system. Both transgenic lines also showed an increase of GUS activity after cold stress, heat stress, metal toxicity, and oxidative damages, suggesting a common induction mechanism in response to the tested stresses including Al stress.

Original languageEnglish
Pages (from-to)1672-1682
Number of pages11
JournalPlant Physiology
Volume134
Issue number4
DOIs
Publication statusPublished - Apr 2004

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Glucuronidase
Glutathione Transferase
Aluminum
glutathione transferase
Arabidopsis
aluminum
Gene Expression
gene expression
genes
genetically modified organisms
shoots
Genes
toxicity
cold stress
ecotypes
reporter genes
heat stress
leaves
stress response
Ecotype

ASJC Scopus subject areas

  • Plant Science

Cite this

Mechanism of gene expression of arabidopsis glutathione S-transferase, AtGST1, and AtGST11 in response to aluminum stress. / Ezaki, Bunichi; Suzuki, Masakatsu; Motoda, Hirotoshi; Kawamura, Masako; Nakashima, Susumu; Matsumoto, Hideaki.

In: Plant Physiology, Vol. 134, No. 4, 04.2004, p. 1672-1682.

Research output: Contribution to journalArticle

Ezaki, B, Suzuki, M, Motoda, H, Kawamura, M, Nakashima, S & Matsumoto, H 2004, 'Mechanism of gene expression of arabidopsis glutathione S-transferase, AtGST1, and AtGST11 in response to aluminum stress', Plant Physiology, vol. 134, no. 4, pp. 1672-1682. https://doi.org/10.1104/pp.103.037135
Ezaki, Bunichi ; Suzuki, Masakatsu ; Motoda, Hirotoshi ; Kawamura, Masako ; Nakashima, Susumu ; Matsumoto, Hideaki. / Mechanism of gene expression of arabidopsis glutathione S-transferase, AtGST1, and AtGST11 in response to aluminum stress. In: Plant Physiology. 2004 ; Vol. 134, No. 4. pp. 1672-1682.
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