Abstract
Dibucaine, a local anesthetic, inhibited the growth of promyelocytic leukemia cells (HL-60) without inducing arrest of the cell cycle and differentiation to granulocytes. Typical DNA fragmentation and DNA ladder formation were induced in a concentration- and time-dependent manner. The half-maximal concentration of dibucaine required to induce apoptosis was 100 μM. These effects were prevented completely by the pan-caspase inhibitor z- Val-Ala-Asp-(OMe)-fluoromethylketone (z-VAD-fmk), thereby implicating the cysteine aspartase (caspase) cascade in the process. Dibucaine activated various caspases, such as caspase-3, -6, -8, and -9 (-like) activities, but not caspase-1 (-like) activity, and induced mitochondrial membrane depolarization and the release of cytochrome c (Cyt.c) from mitochondria into the cytosol. Processing of pro-caspase-3, -8, and -9 by dibucaine was confirmed by western blot analysis. Bid, a death agonist member of the Bcl-2 family, was processed by caspases following exposure of cells to dibucaine. However, 100 μM dibucaine scarcely inhibited oxidative phosphorylation, but it induced membrane permeability transition in isolated rat liver mitochondria. Taken together, these data suggest that dibucaine induced apoptosis of HL-60 cells through activation of the caspase cascade in conjunction with Cyt.c release induced by a processed product of Bid and depolarization of the mitochondrial membrane potential. (C) 2000 Elsevier Science Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 905-915 |
| Number of pages | 11 |
| Journal | Biochemical Pharmacology |
| Volume | 60 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Oct 1 2000 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Bid
- Caspase activation
- Cytochrome c
- Dibucaine
- Membrane permeability transition
ASJC Scopus subject areas
- Pharmacology
Cite this
Mechanism of dibucaine-induced apoptosis in promyelocytic leukemia cells (HL-60). / Arita, Kayo; Utsumi, Toshihiko; Kato, Akio; Kanno, Tomoko; Kobuchi, Hirotsugu; Inoue, Bunji; Akiyama, Jitsuo; Utsumi, Kozo.
In: Biochemical Pharmacology, Vol. 60, No. 7, 01.10.2000, p. 905-915.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanism of dibucaine-induced apoptosis in promyelocytic leukemia cells (HL-60)
AU - Arita, Kayo
AU - Utsumi, Toshihiko
AU - Kato, Akio
AU - Kanno, Tomoko
AU - Kobuchi, Hirotsugu
AU - Inoue, Bunji
AU - Akiyama, Jitsuo
AU - Utsumi, Kozo
PY - 2000/10/1
Y1 - 2000/10/1
N2 - Dibucaine, a local anesthetic, inhibited the growth of promyelocytic leukemia cells (HL-60) without inducing arrest of the cell cycle and differentiation to granulocytes. Typical DNA fragmentation and DNA ladder formation were induced in a concentration- and time-dependent manner. The half-maximal concentration of dibucaine required to induce apoptosis was 100 μM. These effects were prevented completely by the pan-caspase inhibitor z- Val-Ala-Asp-(OMe)-fluoromethylketone (z-VAD-fmk), thereby implicating the cysteine aspartase (caspase) cascade in the process. Dibucaine activated various caspases, such as caspase-3, -6, -8, and -9 (-like) activities, but not caspase-1 (-like) activity, and induced mitochondrial membrane depolarization and the release of cytochrome c (Cyt.c) from mitochondria into the cytosol. Processing of pro-caspase-3, -8, and -9 by dibucaine was confirmed by western blot analysis. Bid, a death agonist member of the Bcl-2 family, was processed by caspases following exposure of cells to dibucaine. However, 100 μM dibucaine scarcely inhibited oxidative phosphorylation, but it induced membrane permeability transition in isolated rat liver mitochondria. Taken together, these data suggest that dibucaine induced apoptosis of HL-60 cells through activation of the caspase cascade in conjunction with Cyt.c release induced by a processed product of Bid and depolarization of the mitochondrial membrane potential. (C) 2000 Elsevier Science Inc.
AB - Dibucaine, a local anesthetic, inhibited the growth of promyelocytic leukemia cells (HL-60) without inducing arrest of the cell cycle and differentiation to granulocytes. Typical DNA fragmentation and DNA ladder formation were induced in a concentration- and time-dependent manner. The half-maximal concentration of dibucaine required to induce apoptosis was 100 μM. These effects were prevented completely by the pan-caspase inhibitor z- Val-Ala-Asp-(OMe)-fluoromethylketone (z-VAD-fmk), thereby implicating the cysteine aspartase (caspase) cascade in the process. Dibucaine activated various caspases, such as caspase-3, -6, -8, and -9 (-like) activities, but not caspase-1 (-like) activity, and induced mitochondrial membrane depolarization and the release of cytochrome c (Cyt.c) from mitochondria into the cytosol. Processing of pro-caspase-3, -8, and -9 by dibucaine was confirmed by western blot analysis. Bid, a death agonist member of the Bcl-2 family, was processed by caspases following exposure of cells to dibucaine. However, 100 μM dibucaine scarcely inhibited oxidative phosphorylation, but it induced membrane permeability transition in isolated rat liver mitochondria. Taken together, these data suggest that dibucaine induced apoptosis of HL-60 cells through activation of the caspase cascade in conjunction with Cyt.c release induced by a processed product of Bid and depolarization of the mitochondrial membrane potential. (C) 2000 Elsevier Science Inc.
KW - Apoptosis
KW - Bid
KW - Caspase activation
KW - Cytochrome c
KW - Dibucaine
KW - Membrane permeability transition
UR - http://www.scopus.com/inward/record.url?scp=0034308206&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034308206&partnerID=8YFLogxK
U2 - 10.1016/S0006-2952(00)00406-8
DO - 10.1016/S0006-2952(00)00406-8
M3 - Article
C2 - 10974198
AN - SCOPUS:0034308206
VL - 60
SP - 905
EP - 915
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 7
ER -