Mechanism of Cu- and Cd-Induced Proline Hyperaccumulation in Triticum aestivum (Wheat)

Bhumi Nath Tripathi, Vijeta Singh, Bunichi Ezaki, Vinay Sharma, J. P. Gaur

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Proline hyperaccumulation, a frequently reported phenomenon in the plant kingdom, is considered an adaptive strategy of plants to cope with a variety of stresses. The present study demonstrated the mechanism of proline hyperaccumulation in Triticum aestivum exposed to excess Cu and Cd. The intracellular level of proline was increased significantly in the plants treated with test metals. Enhanced proline accumulation due to Cu and Cd was negatively correlated with the growth of the treated plants, indicating that plants divert their resources toward induction of survival strategies rather than improving growth and development. The activities of pyrroline-5-carboxylate synthetase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) were increased immediately after metal treatment and remained higher through the end of the experiment, whereas ornithine amino transferase (OAT) activity of the treated plants was lower than P5CS and P5CR enzymes. The activity of proline dehydrogenase (PDH) was decreased sharply in the early phase of metal exposure (up to 12 h) but remained unchanged thereafter until the end of the experiment. Expression analysis of the transcripts of P5CS, OAT, and PDH also supports the above findings and indicates the tissue-specific expression of these genes. Based on these results, increased proline synthesis through the glutamate pathway involving the P5CS and P5CR enzymes is a major contributor to proline hyperaccumulation followed by decreased catabolism of proline.

Original languageEnglish
Pages (from-to)799-808
Number of pages10
JournalJournal of Plant Growth Regulation
Volume32
Issue number4
DOIs
Publication statusPublished - Dec 2013

Fingerprint

proline
Triticum aestivum
wheat
ligases
ornithine-oxo-acid transaminase
metals
enzymes
transcriptomics
glutamates
growth and development
gene expression
metabolism
synthesis

Keywords

  • Cadmium
  • Copper
  • Proline
  • Wheat

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Mechanism of Cu- and Cd-Induced Proline Hyperaccumulation in Triticum aestivum (Wheat). / Tripathi, Bhumi Nath; Singh, Vijeta; Ezaki, Bunichi; Sharma, Vinay; Gaur, J. P.

In: Journal of Plant Growth Regulation, Vol. 32, No. 4, 12.2013, p. 799-808.

Research output: Contribution to journalArticle

Tripathi, Bhumi Nath ; Singh, Vijeta ; Ezaki, Bunichi ; Sharma, Vinay ; Gaur, J. P. / Mechanism of Cu- and Cd-Induced Proline Hyperaccumulation in Triticum aestivum (Wheat). In: Journal of Plant Growth Regulation. 2013 ; Vol. 32, No. 4. pp. 799-808.
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