Mechanism of calcium‐dependent salt tolerance in cells of Nitellopsis obtusa: role of intracellular adenine nucleotides

Maki Katsuhara, M. TAZAWA

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Abstract. The tonoplasts of internodal cells of Nitellopsis were removed by perfusing the vacuoles with media containing a Ca2 chelator, EGTA. Treatment of tonoplast‐free cells with 100 mol m3 NaCl induces a large membrane depolarization, a drastic decrease in the membrane resistance and an increase in Na+ influx. These events are identical to those that occur in intact cells subjected to high NaCl. These responses to NaCl are prevented if 10 mol m3 Ca2+ is supplied together with 100 mol m3 NaCl. The protective effect of Ca2+ is evident only when the intracellular ATP concentration exceeds 0.1 mol m3 and does not occur full when the intracellular ATP is removed. AMP at concentrations greater than 0.5 mol m3 or 0.25 mol m3 AMPPNP can replace ATP. It is concluded that ATP does not act as an energy source nor as a substrate for protein phosphorylation. ATP seems to exert its effects as a coeffector with Ca2+ in regulating the Na+ permeability of the plasma membrane.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalPlant, Cell and Environment
Volume13
Issue number2
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Salt-Tolerance
Adenine Nucleotides
salt tolerance
Adenosine Triphosphate
calcium
cells
Adenylyl Imidodiphosphate
protein phosphorylation
tonoplast
chelating agents
Membranes
Egtazic Acid
vacuoles
protective effect
Adenosine Monophosphate
Chelating Agents
permeability
Vacuoles
plasma membrane
Permeability

Keywords

  • ATP
  • calcium
  • charophyte
  • effector
  • Nitellopsis obtusa
  • salt stress
  • salt tolerance

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

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abstract = "Abstract. The tonoplasts of internodal cells of Nitellopsis were removed by perfusing the vacuoles with media containing a Ca2 chelator, EGTA. Treatment of tonoplast‐free cells with 100 mol m3 NaCl induces a large membrane depolarization, a drastic decrease in the membrane resistance and an increase in Na+ influx. These events are identical to those that occur in intact cells subjected to high NaCl. These responses to NaCl are prevented if 10 mol m3 Ca2+ is supplied together with 100 mol m3 NaCl. The protective effect of Ca2+ is evident only when the intracellular ATP concentration exceeds 0.1 mol m3 and does not occur full when the intracellular ATP is removed. AMP at concentrations greater than 0.5 mol m3 or 0.25 mol m3 AMPPNP can replace ATP. It is concluded that ATP does not act as an energy source nor as a substrate for protein phosphorylation. ATP seems to exert its effects as a coeffector with Ca2+ in regulating the Na+ permeability of the plasma membrane.",
keywords = "ATP, calcium, charophyte, effector, Nitellopsis obtusa, salt stress, salt tolerance",
author = "Maki Katsuhara and M. TAZAWA",
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T2 - role of intracellular adenine nucleotides

AU - Katsuhara, Maki

AU - TAZAWA, M.

PY - 1990

Y1 - 1990

N2 - Abstract. The tonoplasts of internodal cells of Nitellopsis were removed by perfusing the vacuoles with media containing a Ca2 chelator, EGTA. Treatment of tonoplast‐free cells with 100 mol m3 NaCl induces a large membrane depolarization, a drastic decrease in the membrane resistance and an increase in Na+ influx. These events are identical to those that occur in intact cells subjected to high NaCl. These responses to NaCl are prevented if 10 mol m3 Ca2+ is supplied together with 100 mol m3 NaCl. The protective effect of Ca2+ is evident only when the intracellular ATP concentration exceeds 0.1 mol m3 and does not occur full when the intracellular ATP is removed. AMP at concentrations greater than 0.5 mol m3 or 0.25 mol m3 AMPPNP can replace ATP. It is concluded that ATP does not act as an energy source nor as a substrate for protein phosphorylation. ATP seems to exert its effects as a coeffector with Ca2+ in regulating the Na+ permeability of the plasma membrane.

AB - Abstract. The tonoplasts of internodal cells of Nitellopsis were removed by perfusing the vacuoles with media containing a Ca2 chelator, EGTA. Treatment of tonoplast‐free cells with 100 mol m3 NaCl induces a large membrane depolarization, a drastic decrease in the membrane resistance and an increase in Na+ influx. These events are identical to those that occur in intact cells subjected to high NaCl. These responses to NaCl are prevented if 10 mol m3 Ca2+ is supplied together with 100 mol m3 NaCl. The protective effect of Ca2+ is evident only when the intracellular ATP concentration exceeds 0.1 mol m3 and does not occur full when the intracellular ATP is removed. AMP at concentrations greater than 0.5 mol m3 or 0.25 mol m3 AMPPNP can replace ATP. It is concluded that ATP does not act as an energy source nor as a substrate for protein phosphorylation. ATP seems to exert its effects as a coeffector with Ca2+ in regulating the Na+ permeability of the plasma membrane.

KW - ATP

KW - calcium

KW - charophyte

KW - effector

KW - Nitellopsis obtusa

KW - salt stress

KW - salt tolerance

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