Abstract
Bradykinin at concentrations higher than 2 μM caused a significant histamine release from rat peritoneal mast cells when extracellular Ca2+ was removed from the medium. Under the same experimental conditions, bradykinin increased Ca2+ release from the intracellular Ca store of the rat peritoneal mast cells, and a clear relationship was observed between the magnitude of histamine release and an increase in fluorescence intensity. Addition of Ca2+ to the medium resulted in an inhibition of the response to bradykinin in a concentration-dependent manner. Almost the same results were obtained when Mg2+, Ba2+ and La3+ were added to the medium. Neither B1 nor B2 antagonists caused significant antagonistic effects on histamine release induced by bradykinin. However, B2 antagonists caused a histamine release of the same potency as bradykinin when applied alone. These results indicate that bradykinin-induced histamine release is not attributable to a bradykinin receptor.
Original language | English |
---|---|
Pages (from-to) | 237-240 |
Number of pages | 4 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 19 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 1996 |
Keywords
- bradykinin
- histamine release
- intracellular Ca release
- mast cell
ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science