Abstract
Bradykinin at concentrations higher than 2 μM caused a significant histamine release from rat peritoneal mast cells when extracellular Ca2+ was removed from the medium. Under the same experimental conditions, bradykinin increased Ca2+ release from the intracellular Ca store of the rat peritoneal mast cells, and a clear relationship was observed between the magnitude of histamine release and an increase in fluorescence intensity. Addition of Ca2+ to the medium resulted in an inhibition of the response to bradykinin in a concentration-dependent manner. Almost the same results were obtained when Mg2+, Ba2+ and La3+ were added to the medium. Neither B1 nor B2 antagonists caused significant antagonistic effects on histamine release induced by bradykinin. However, B2 antagonists caused a histamine release of the same potency as bradykinin when applied alone. These results indicate that bradykinin-induced histamine release is not attributable to a bradykinin receptor.
| Original language | English |
|---|---|
| Pages (from-to) | 237-240 |
| Number of pages | 4 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 19 |
| Issue number | 2 |
| Publication status | Published - Feb 1996 |
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Keywords
- bradykinin
- histamine release
- intracellular Ca release
- mast cell
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Mechanism of bradykinin-induced histamine release from rat peritoneal mast cells. / Zhao, Qiu E.; Mihara, Takuma; Sugimoto, Yukio; Kamei, Chiaki.
In: Biological and Pharmaceutical Bulletin, Vol. 19, No. 2, 02.1996, p. 237-240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanism of bradykinin-induced histamine release from rat peritoneal mast cells
AU - Zhao, Qiu E.
AU - Mihara, Takuma
AU - Sugimoto, Yukio
AU - Kamei, Chiaki
PY - 1996/2
Y1 - 1996/2
N2 - Bradykinin at concentrations higher than 2 μM caused a significant histamine release from rat peritoneal mast cells when extracellular Ca2+ was removed from the medium. Under the same experimental conditions, bradykinin increased Ca2+ release from the intracellular Ca store of the rat peritoneal mast cells, and a clear relationship was observed between the magnitude of histamine release and an increase in fluorescence intensity. Addition of Ca2+ to the medium resulted in an inhibition of the response to bradykinin in a concentration-dependent manner. Almost the same results were obtained when Mg2+, Ba2+ and La3+ were added to the medium. Neither B1 nor B2 antagonists caused significant antagonistic effects on histamine release induced by bradykinin. However, B2 antagonists caused a histamine release of the same potency as bradykinin when applied alone. These results indicate that bradykinin-induced histamine release is not attributable to a bradykinin receptor.
AB - Bradykinin at concentrations higher than 2 μM caused a significant histamine release from rat peritoneal mast cells when extracellular Ca2+ was removed from the medium. Under the same experimental conditions, bradykinin increased Ca2+ release from the intracellular Ca store of the rat peritoneal mast cells, and a clear relationship was observed between the magnitude of histamine release and an increase in fluorescence intensity. Addition of Ca2+ to the medium resulted in an inhibition of the response to bradykinin in a concentration-dependent manner. Almost the same results were obtained when Mg2+, Ba2+ and La3+ were added to the medium. Neither B1 nor B2 antagonists caused significant antagonistic effects on histamine release induced by bradykinin. However, B2 antagonists caused a histamine release of the same potency as bradykinin when applied alone. These results indicate that bradykinin-induced histamine release is not attributable to a bradykinin receptor.
KW - bradykinin
KW - histamine release
KW - intracellular Ca release
KW - mast cell
UR - http://www.scopus.com/inward/record.url?scp=0029919368&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029919368&partnerID=8YFLogxK
M3 - Article
VL - 19
SP - 237
EP - 240
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 2
ER -