Mechanism of arachidonic acid liberation during ischemia in gerbil cerebral cortex

K. Abe, K. Kogure, H. Yamamoto, M. Imazawa, K. Miyamoto

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Once brain ischemia was induced in the gerbil cerebral fronto-parietal cortex, serial changes occurred in energy metabolites and various lipids. The amounts of inositol-containing phospholipids began to decrease immediately after energy failure, followed by an increase in the amount of 1,2-diacylglycerol with a subsequent liberation of arachidonic acid and other free fatty acids. The fatty acid compositions of inositol-containing phospholipids, of 1,2-diacylglycerols produced by ischemia, and of free fatty acids liberated during ischemia were quite similar. The amount of stearic acid liberated was much larger than that of arachidonic acid between 30 s and 1 min of ischemia. On the other hand, there was no significant decrease in the amount of the other phospholipids except for phosphatidic acid. Furthermore, there was also no change in the fatty acid composition of phosphatidylcholine or phosphatidylethanolamine throughout 15 min of ischemia. The amount of cytidine-monophosphate reached a peak (36.7 nmol/g wet wt) at 2 min of ischemia. These results indicated that arachidonic acid was predominantly liberated from inositol-containing phospholipids by phospholipase C, and by the diglyceride lipase and monoglyceride lipase system rather than from phosphatidylcholine or phosphatidylethanolamine by phospholipase A2 or plasmalogenase or choline phosphotransferase during the early period of ischemia.

Original languageEnglish
Pages (from-to)503-509
Number of pages7
JournalJournal of Neurochemistry
Volume48
Issue number2
DOIs
Publication statusPublished - 1987
Externally publishedYes

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Gerbillinae
Arachidonic Acid
Cerebral Cortex
Phospholipids
Inositol
Ischemia
Phosphatidylinositols
Phosphatidylcholines
Nonesterified Fatty Acids
Fatty Acids
Monoacylglycerol Lipases
Cytidine Monophosphate
Phosphatidic Acids
Lipoprotein Lipase
Phospholipases A2
Type C Phospholipases
Metabolites
Choline
Chemical analysis
Brain

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Mechanism of arachidonic acid liberation during ischemia in gerbil cerebral cortex. / Abe, K.; Kogure, K.; Yamamoto, H.; Imazawa, M.; Miyamoto, K.

In: Journal of Neurochemistry, Vol. 48, No. 2, 1987, p. 503-509.

Research output: Contribution to journalArticle

Abe, K. ; Kogure, K. ; Yamamoto, H. ; Imazawa, M. ; Miyamoto, K. / Mechanism of arachidonic acid liberation during ischemia in gerbil cerebral cortex. In: Journal of Neurochemistry. 1987 ; Vol. 48, No. 2. pp. 503-509.
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