Mechanism of 3-nitropropionic acid-induced membrane permeability transition of isolated mitochondria and its suppression by L-carnitine.

Makoto Nishimura, Yuya Okimura, Hirofumi Fujita, Hiromi Yano, Jintae Lee, Etsuko Suzaki, Masayasu Inoue, Kozo Utsumi, Junzo Sasaki

Research output: Contribution to journalArticle

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Abstract

3-Nitropropionic acid (3NP) functions as an irreversible inhibitor of succinic acid dehydrogenase (complex II) and induces neuronal disorders in rats similar to those in patients with Huntington's disease. It is well known that L-carnitine (LC), a carrier of long chain fatty acid into the mitochondrial matrix, attenuates the neuronal degeneration in 3NP-treated rats. From these findings it has been suggested that 3NP induces certain neuronal cell death through mitochondrial dysfunction and that LC preserves the neurons against the dysfunction of mitochondria caused by 3NP. However, the detailed mechanism of cell death by 3NP and the protective actions of LC against the mitochondrial dysfunction have not been fully elucidated yet. Thus, we studied the molecular mechanism of the effects of 3NP and LC on isolated rat liver mitochondria. 3NP inhibited succinate respiration and the decreased respiratory control ratio of isolated mitochondria without affecting oxidative phosphorylation. 3NP induced a membrane permeability transition (MPT), which plays an important role in the mechanism of apoptotic cell death. 3NP stimulated Ca2+ release from mitochondria, decreased membrane potential, induced mitochondrial swelling, and stimulated cytochrome c release from mitochondria. 3NP-induced swelling was suppressed by bovine serum albumin, inhibitors of phospholipase A(2) and by an inhibitor of classic MPT, cyclosporin A. Furthermore, LC suppressed the changes brought about by 3NP in mitochondrial functions in the presence of ATP. These results suggest that MPT underlies the mechanism of 3NP-induced cell death, and that LC attenuates mitochondrial MPT by decreasing long chain fatty acids generated by phospholipase A(2).

Original languageEnglish
Pages (from-to)881-891
Number of pages11
JournalCell Biochemistry and Function
Volume26
Issue number8
DOIs
Publication statusPublished - Dec 2008

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Mitochondria
Carnitine
Permeability
Membranes
Cell death
Cell Death
Rats
Phospholipases A
Succinic Acid
Swelling
3-nitropropionic acid
Fatty Acids
Mitochondrial Swelling
Liver Mitochondrion
Huntington Disease
Oxidative Phosphorylation
Mitochondrial Membranes
Bovine Serum Albumin
Cytochromes c
Liver

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Mechanism of 3-nitropropionic acid-induced membrane permeability transition of isolated mitochondria and its suppression by L-carnitine. / Nishimura, Makoto; Okimura, Yuya; Fujita, Hirofumi; Yano, Hiromi; Lee, Jintae; Suzaki, Etsuko; Inoue, Masayasu; Utsumi, Kozo; Sasaki, Junzo.

In: Cell Biochemistry and Function, Vol. 26, No. 8, 12.2008, p. 881-891.

Research output: Contribution to journalArticle

Nishimura, Makoto ; Okimura, Yuya ; Fujita, Hirofumi ; Yano, Hiromi ; Lee, Jintae ; Suzaki, Etsuko ; Inoue, Masayasu ; Utsumi, Kozo ; Sasaki, Junzo. / Mechanism of 3-nitropropionic acid-induced membrane permeability transition of isolated mitochondria and its suppression by L-carnitine. In: Cell Biochemistry and Function. 2008 ; Vol. 26, No. 8. pp. 881-891.
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