TY - JOUR
T1 - Mechanical stimulation of gingiva reduces plasma 8-OHdG level in rat periodontitis
AU - Ekuni, Daisuke
AU - Tomofuji, Takaaki
AU - Tamaki, Naofumi
AU - Sanbe, Toshihiro
AU - Azuma, Tetsuji
AU - Yamanaka, Reiko
AU - Yamamoto, Tatsuo
AU - Watanabe, Tatsuo
N1 - Funding Information:
This study was supported by Grants-in-Aid for Scientific Research (Nos. 17209066, 17791576, 18592277, 18791612) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2008/4
Y1 - 2008/4
N2 - Objective: Gingival cells respond to periodontal pathogens by generating reactive oxygen species, and such a condition would increase circulating oxidative stress. Improvement of gingival inflammation by toothbrushing may offer clinical benefits on not only periodontal health but also the circulatory conditions. We examined the effects of mechanical stimulation on the plasma 8-hydroxydeoxyguanosine level in a rat periodontitis model. Design: In this experiment, male Wistar rats (n = 18) were divided into three groups. The control group received topical application of pyrogen-free water to the gingival sulcus for 8 weeks, while the other two groups received topical application of bacterial pathogens (lipopolysaccharide and proteases). After 4 weeks, half of the rats in the experimental groups received daily mechanical stimulation with an electric toothbrush for 4 weeks. Results: Rats treated with bacterial pathogens presented periodontal tissue damage and increased plasma levels of 8-hydroxydeoxyguanosine. Mechanical stimulation by toothbrushing decreased gingival inflammation and oxidative DNA damage indicated by a decrease in plasma 8-hydroxydeoxyguanosine. Conclusions: Mechanical stimulation of periodontally involved gingiva reduced 8-hydroxydeoxyguanosine in plasma and may contribute to a reduction in circulating oxidative stress associated molecules.
AB - Objective: Gingival cells respond to periodontal pathogens by generating reactive oxygen species, and such a condition would increase circulating oxidative stress. Improvement of gingival inflammation by toothbrushing may offer clinical benefits on not only periodontal health but also the circulatory conditions. We examined the effects of mechanical stimulation on the plasma 8-hydroxydeoxyguanosine level in a rat periodontitis model. Design: In this experiment, male Wistar rats (n = 18) were divided into three groups. The control group received topical application of pyrogen-free water to the gingival sulcus for 8 weeks, while the other two groups received topical application of bacterial pathogens (lipopolysaccharide and proteases). After 4 weeks, half of the rats in the experimental groups received daily mechanical stimulation with an electric toothbrush for 4 weeks. Results: Rats treated with bacterial pathogens presented periodontal tissue damage and increased plasma levels of 8-hydroxydeoxyguanosine. Mechanical stimulation by toothbrushing decreased gingival inflammation and oxidative DNA damage indicated by a decrease in plasma 8-hydroxydeoxyguanosine. Conclusions: Mechanical stimulation of periodontally involved gingiva reduced 8-hydroxydeoxyguanosine in plasma and may contribute to a reduction in circulating oxidative stress associated molecules.
KW - Lipopolysaccharide
KW - Mechanical stimulation
KW - Oxidative stress
KW - Periodontal diseases
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U2 - 10.1016/j.archoralbio.2007.10.005
DO - 10.1016/j.archoralbio.2007.10.005
M3 - Article
C2 - 18031711
AN - SCOPUS:39749151195
VL - 53
SP - 324
EP - 329
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 4
ER -