TY - JOUR
T1 - Mechanical and biocompatibility properties of calcium phosphate bioceramics derived from salmon fish bone wastes
AU - Bas, Merve
AU - Daglilar, Sibel
AU - Kuskonmaz, Nilgun
AU - Kalkandelen, Cevriye
AU - Erdemir, Gokce
AU - Kuruca, Serap E.
AU - Tulyaganov, Dilshat
AU - Yoshioka, Tomohiko
AU - Gunduz, Oguzhan
AU - Ficai, Denisa
AU - Ficai, Anton
N1 - Funding Information:
Funding: This work has been supported by Marmara University Scientific Research Projects Coordination Unit under grant number FEN-B-121218-0614.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Natural calcium phosphates derived from fish wastes are a promising material for biomedical application. However, their sintered ceramics are not fully characterized in terms of mechanical and biological properties. In this study, natural calcium phosphate was synthesized through a thermal calcination process from salmon fish bone wastes. The salmon-derived calcium phosphates (sCaP) were sintered at different temperatures to obtain natural calcium phosphate bioceramics and then were investigated in terms of their microstructure, mechanical properties and biocompatibility. In particular, this work is concerned with the effects of grain size on the relative density and microhardness of the sCaP bioceramics. Ca/P ratio of the sintered sCaP ranged from 1.73 to 1.52 when the sintering temperature was raised from 1000 to 1300◦ C. The crystal phase of all the sCaP bioceramics obtained was biphasic and composed of hydroxyapatite (HA) and tricalcium phosphate (TCP). The density and microhardness of the sCaP bioceramics increased in the temperature interval 1000–1100◦ C, while at temperatures higher than 1100◦ C, these properties were not significantly altered. The highest compressive strength of 116 MPa was recorded for the samples sintered at 1100◦ C. In vitro biocompatibility was also examined in the behavior of osteosarcoma (Saos-2) cells, indicating that the sCaP bioceramics had no cytotoxicity effect. Salmon-derived biphasic calcium phosphates (BCP) have the potential to contribute to the development of bone substituted materials.
AB - Natural calcium phosphates derived from fish wastes are a promising material for biomedical application. However, their sintered ceramics are not fully characterized in terms of mechanical and biological properties. In this study, natural calcium phosphate was synthesized through a thermal calcination process from salmon fish bone wastes. The salmon-derived calcium phosphates (sCaP) were sintered at different temperatures to obtain natural calcium phosphate bioceramics and then were investigated in terms of their microstructure, mechanical properties and biocompatibility. In particular, this work is concerned with the effects of grain size on the relative density and microhardness of the sCaP bioceramics. Ca/P ratio of the sintered sCaP ranged from 1.73 to 1.52 when the sintering temperature was raised from 1000 to 1300◦ C. The crystal phase of all the sCaP bioceramics obtained was biphasic and composed of hydroxyapatite (HA) and tricalcium phosphate (TCP). The density and microhardness of the sCaP bioceramics increased in the temperature interval 1000–1100◦ C, while at temperatures higher than 1100◦ C, these properties were not significantly altered. The highest compressive strength of 116 MPa was recorded for the samples sintered at 1100◦ C. In vitro biocompatibility was also examined in the behavior of osteosarcoma (Saos-2) cells, indicating that the sCaP bioceramics had no cytotoxicity effect. Salmon-derived biphasic calcium phosphates (BCP) have the potential to contribute to the development of bone substituted materials.
KW - Hydroxyapatite
KW - Mechanical properties
KW - Salmon fish bone
KW - Sintering
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U2 - 10.3390/ijms21218082
DO - 10.3390/ijms21218082
M3 - Article
C2 - 33138182
AN - SCOPUS:85095395906
VL - 21
SP - 1
EP - 14
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 21
M1 - 8082
ER -