Abstract
The solubilities of phenylalanine and tyrosine in supercritical carbon dioxide (SCCO2) were measured after derivatization as the N-acetyl amino acid ethyl ester, N-carbobenzoxy amino acid and N-acetyl amino acid. Using an SCCO2 flow system, a measuring method of the saturated solubilities of the derivatized amino acids was established in which the contact height of the extraction cell, i.e. a packed column, is increased till the concentration of a derivatized amino acid at the exit of the cell reaches a plateau. The solubilities of N-acetyl phenylalanine ethyl ester (APEE) exceeded 0.001 mole fraction, which is higher than those of caffeine produced in industrial SCCO2 processes. A possible way of separating the amino acid mixtures using polarity differences in different amino acid side chains was demonstrated using the solubility data of the N-acetyl-amino acid ethyl esters in SCCO2, as the solubilities of APEE are higher than those of N-acetyl tyrosine ethyl ester by two orders of magnitude.
| Original language | English |
|---|---|
| Pages (from-to) | 201-206 |
| Number of pages | 6 |
| Journal | Fluid Phase Equilibria |
| Volume | 257 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 25 2007 |
| Externally published | Yes |
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Keywords
- Amino acid derivative
- Flow system
- Solubility
- Supercritical carbon dioxide
ASJC Scopus subject areas
- Fluid Flow and Transfer Processes
- Physical and Theoretical Chemistry
Cite this
Measurements of the solubilities of derivatized amino acids in supercritical carbon dioxide. / Freitag, Joerg; Yokoyama, You; Kuramochi, Hidetoshi; Kawamoto, Katsuya; Nagahama, Kunio; Kato, Satoru.
In: Fluid Phase Equilibria, Vol. 257, No. 2, 25.08.2007, p. 201-206.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurements of the solubilities of derivatized amino acids in supercritical carbon dioxide
AU - Freitag, Joerg
AU - Yokoyama, You
AU - Kuramochi, Hidetoshi
AU - Kawamoto, Katsuya
AU - Nagahama, Kunio
AU - Kato, Satoru
PY - 2007/8/25
Y1 - 2007/8/25
N2 - The solubilities of phenylalanine and tyrosine in supercritical carbon dioxide (SCCO2) were measured after derivatization as the N-acetyl amino acid ethyl ester, N-carbobenzoxy amino acid and N-acetyl amino acid. Using an SCCO2 flow system, a measuring method of the saturated solubilities of the derivatized amino acids was established in which the contact height of the extraction cell, i.e. a packed column, is increased till the concentration of a derivatized amino acid at the exit of the cell reaches a plateau. The solubilities of N-acetyl phenylalanine ethyl ester (APEE) exceeded 0.001 mole fraction, which is higher than those of caffeine produced in industrial SCCO2 processes. A possible way of separating the amino acid mixtures using polarity differences in different amino acid side chains was demonstrated using the solubility data of the N-acetyl-amino acid ethyl esters in SCCO2, as the solubilities of APEE are higher than those of N-acetyl tyrosine ethyl ester by two orders of magnitude.
AB - The solubilities of phenylalanine and tyrosine in supercritical carbon dioxide (SCCO2) were measured after derivatization as the N-acetyl amino acid ethyl ester, N-carbobenzoxy amino acid and N-acetyl amino acid. Using an SCCO2 flow system, a measuring method of the saturated solubilities of the derivatized amino acids was established in which the contact height of the extraction cell, i.e. a packed column, is increased till the concentration of a derivatized amino acid at the exit of the cell reaches a plateau. The solubilities of N-acetyl phenylalanine ethyl ester (APEE) exceeded 0.001 mole fraction, which is higher than those of caffeine produced in industrial SCCO2 processes. A possible way of separating the amino acid mixtures using polarity differences in different amino acid side chains was demonstrated using the solubility data of the N-acetyl-amino acid ethyl esters in SCCO2, as the solubilities of APEE are higher than those of N-acetyl tyrosine ethyl ester by two orders of magnitude.
KW - Amino acid derivative
KW - Flow system
KW - Solubility
KW - Supercritical carbon dioxide
UR - http://www.scopus.com/inward/record.url?scp=34250700547&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250700547&partnerID=8YFLogxK
U2 - 10.1016/j.fluid.2007.01.032
DO - 10.1016/j.fluid.2007.01.032
M3 - Article
AN - SCOPUS:34250700547
VL - 257
SP - 201
EP - 206
JO - Fluid Phase Equilibria
JF - Fluid Phase Equilibria
SN - 0378-3812
IS - 2
ER -