Measurements of the solubilities of derivatized amino acids in supercritical carbon dioxide

Joerg Freitag, You Yokoyama, Hidetoshi Kuramochi, Katsuya Kawamoto, Kunio Nagahama, Satoru Kato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The solubilities of phenylalanine and tyrosine in supercritical carbon dioxide (SCCO2) were measured after derivatization as the N-acetyl amino acid ethyl ester, N-carbobenzoxy amino acid and N-acetyl amino acid. Using an SCCO2 flow system, a measuring method of the saturated solubilities of the derivatized amino acids was established in which the contact height of the extraction cell, i.e. a packed column, is increased till the concentration of a derivatized amino acid at the exit of the cell reaches a plateau. The solubilities of N-acetyl phenylalanine ethyl ester (APEE) exceeded 0.001 mole fraction, which is higher than those of caffeine produced in industrial SCCO2 processes. A possible way of separating the amino acid mixtures using polarity differences in different amino acid side chains was demonstrated using the solubility data of the N-acetyl-amino acid ethyl esters in SCCO2, as the solubilities of APEE are higher than those of N-acetyl tyrosine ethyl ester by two orders of magnitude.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalFluid Phase Equilibria
Volume257
Issue number2
DOIs
Publication statusPublished - Aug 25 2007
Externally publishedYes

Fingerprint

Carbon Dioxide
amino acids
carbon dioxide
Amino acids
Carbon dioxide
solubility
Solubility
Amino Acids
esters
Esters
phenylalanine
Phenylalanine
tyrosine
Caffeine
caffeine
cells
Tyrosine
plateaus
polarity

Keywords

  • Amino acid derivative
  • Flow system
  • Solubility
  • Supercritical carbon dioxide

ASJC Scopus subject areas

  • Fluid Flow and Transfer Processes
  • Physical and Theoretical Chemistry

Cite this

Measurements of the solubilities of derivatized amino acids in supercritical carbon dioxide. / Freitag, Joerg; Yokoyama, You; Kuramochi, Hidetoshi; Kawamoto, Katsuya; Nagahama, Kunio; Kato, Satoru.

In: Fluid Phase Equilibria, Vol. 257, No. 2, 25.08.2007, p. 201-206.

Research output: Contribution to journalArticle

Freitag, Joerg ; Yokoyama, You ; Kuramochi, Hidetoshi ; Kawamoto, Katsuya ; Nagahama, Kunio ; Kato, Satoru. / Measurements of the solubilities of derivatized amino acids in supercritical carbon dioxide. In: Fluid Phase Equilibria. 2007 ; Vol. 257, No. 2. pp. 201-206.
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