Abstract
We have improved a previously described pressure probe in the following respect to make it suitable for continous mesurements of intracelluar presure (p)i in higher plant cells. (1)The oil/cell-sap boundary (meniscus) at the tip of the microcapillary was moniterd photo-electrically with a high-resolution image sensor. (2) Adjustment of the plunger and the insertion of the probe into the target cell were performed with specilly desinged, hydraulic manipulator system. These improvements allowed us to monitor the meniscus precisely and to maintain the meniscus at a certain position without mechanical perturbation. This system has been successfully used to measure Pi continuously for more than 3 h.With this pressure probe, the dependence on respiration of Pi in the elongation zone of Vigna hypocotyls, which was predicted by Katou and Furumoto (1986a, b), was demonstrated directly. Changes in Pi induced by osmotic stress were also measured quite successfully. The respiration-dependent component of Pi was 30-40 kPa. While this component appears to be very small, the change in Pi under anoxia caused shrinkage of the segment of hypocotyl being examined. Regulation of Pu over a range of several tens of kPa or so should, therefore, make a significant contribution to the control of elongation growth.
| Original language | English |
|---|---|
| Pages (from-to) | 859-864 |
| Number of pages | 6 |
| Journal | Plant and Cell Physiology |
| Volume | 31 |
| Issue number | 6 |
| Publication status | Published - 1990 |
| Externally published | Yes |
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Keywords
- Anoxia
- Elongation growth
- Intracellular pressure
- Pressure probe
- Vigna hypocotyl
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Ecology
- Cell Biology
- Physiology
- Plant Science
Cite this
Measurement of the respiration-dependent component of intracellular pressure with an improved pressure probe. / Nakahori, Kiyoshi; Koizumi, Kazuji; Muramatsu, Satoru; Ohtani, Hiroyuki; Masuko, Masayuki; Nakase, Sigeki; Katou, Kiyoshi; Okamoto, Hisashi.
In: Plant and Cell Physiology, Vol. 31, No. 6, 1990, p. 859-864.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of the respiration-dependent component of intracellular pressure with an improved pressure probe
AU - Nakahori, Kiyoshi
AU - Koizumi, Kazuji
AU - Muramatsu, Satoru
AU - Ohtani, Hiroyuki
AU - Masuko, Masayuki
AU - Nakase, Sigeki
AU - Katou, Kiyoshi
AU - Okamoto, Hisashi
PY - 1990
Y1 - 1990
N2 - We have improved a previously described pressure probe in the following respect to make it suitable for continous mesurements of intracelluar presure (p)i in higher plant cells. (1)The oil/cell-sap boundary (meniscus) at the tip of the microcapillary was moniterd photo-electrically with a high-resolution image sensor. (2) Adjustment of the plunger and the insertion of the probe into the target cell were performed with specilly desinged, hydraulic manipulator system. These improvements allowed us to monitor the meniscus precisely and to maintain the meniscus at a certain position without mechanical perturbation. This system has been successfully used to measure Pi continuously for more than 3 h.With this pressure probe, the dependence on respiration of Pi in the elongation zone of Vigna hypocotyls, which was predicted by Katou and Furumoto (1986a, b), was demonstrated directly. Changes in Pi induced by osmotic stress were also measured quite successfully. The respiration-dependent component of Pi was 30-40 kPa. While this component appears to be very small, the change in Pi under anoxia caused shrinkage of the segment of hypocotyl being examined. Regulation of Pu over a range of several tens of kPa or so should, therefore, make a significant contribution to the control of elongation growth.
AB - We have improved a previously described pressure probe in the following respect to make it suitable for continous mesurements of intracelluar presure (p)i in higher plant cells. (1)The oil/cell-sap boundary (meniscus) at the tip of the microcapillary was moniterd photo-electrically with a high-resolution image sensor. (2) Adjustment of the plunger and the insertion of the probe into the target cell were performed with specilly desinged, hydraulic manipulator system. These improvements allowed us to monitor the meniscus precisely and to maintain the meniscus at a certain position without mechanical perturbation. This system has been successfully used to measure Pi continuously for more than 3 h.With this pressure probe, the dependence on respiration of Pi in the elongation zone of Vigna hypocotyls, which was predicted by Katou and Furumoto (1986a, b), was demonstrated directly. Changes in Pi induced by osmotic stress were also measured quite successfully. The respiration-dependent component of Pi was 30-40 kPa. While this component appears to be very small, the change in Pi under anoxia caused shrinkage of the segment of hypocotyl being examined. Regulation of Pu over a range of several tens of kPa or so should, therefore, make a significant contribution to the control of elongation growth.
KW - Anoxia
KW - Elongation growth
KW - Intracellular pressure
KW - Pressure probe
KW - Vigna hypocotyl
UR - http://www.scopus.com/inward/record.url?scp=0008987704&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0008987704&partnerID=8YFLogxK
M3 - Article
VL - 31
SP - 859
EP - 864
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 6
ER -