Measurement of the respiration-dependent component of intracellular pressure with an improved pressure probe

Kiyoshi Nakahori, Kazuji Koizumi, Satoru Muramatsu, Hiroyuki Ohtani, Masayuki Masuko, Sigeki Nakase, Kiyoshi Katou, Hisashi Okamoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have improved a previously described pressure probe in the following respect to make it suitable for continous mesurements of intracelluar presure (p)i in higher plant cells. (1)The oil/cell-sap boundary (meniscus) at the tip of the microcapillary was moniterd photo-electrically with a high-resolution image sensor. (2) Adjustment of the plunger and the insertion of the probe into the target cell were performed with specilly desinged, hydraulic manipulator system. These improvements allowed us to monitor the meniscus precisely and to maintain the meniscus at a certain position without mechanical perturbation. This system has been successfully used to measure Pi continuously for more than 3 h.With this pressure probe, the dependence on respiration of Pi in the elongation zone of Vigna hypocotyls, which was predicted by Katou and Furumoto (1986a, b), was demonstrated directly. Changes in Pi induced by osmotic stress were also measured quite successfully. The respiration-dependent component of Pi was 30-40 kPa. While this component appears to be very small, the change in Pi under anoxia caused shrinkage of the segment of hypocotyl being examined. Regulation of Pu over a range of several tens of kPa or so should, therefore, make a significant contribution to the control of elongation growth.

Original languageEnglish
Pages (from-to)859-864
Number of pages6
JournalPlant and Cell Physiology
Volume31
Issue number6
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Respiration
Pi
cell respiration
probes (equipment)
Hypocotyl
respiration
Probe
probe
Pressure
hypocotyls
Dependent
Elongation
hydraulic systems
Vigna
Osmotic Pressure
Plant Cells
anoxia
cells
image resolution
osmotic stress

Keywords

  • Anoxia
  • Elongation growth
  • Intracellular pressure
  • Pressure probe
  • Vigna hypocotyl

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Nakahori, K., Koizumi, K., Muramatsu, S., Ohtani, H., Masuko, M., Nakase, S., ... Okamoto, H. (1990). Measurement of the respiration-dependent component of intracellular pressure with an improved pressure probe. Plant and Cell Physiology, 31(6), 859-864.

Measurement of the respiration-dependent component of intracellular pressure with an improved pressure probe. / Nakahori, Kiyoshi; Koizumi, Kazuji; Muramatsu, Satoru; Ohtani, Hiroyuki; Masuko, Masayuki; Nakase, Sigeki; Katou, Kiyoshi; Okamoto, Hisashi.

In: Plant and Cell Physiology, Vol. 31, No. 6, 1990, p. 859-864.

Research output: Contribution to journalArticle

Nakahori, K, Koizumi, K, Muramatsu, S, Ohtani, H, Masuko, M, Nakase, S, Katou, K & Okamoto, H 1990, 'Measurement of the respiration-dependent component of intracellular pressure with an improved pressure probe', Plant and Cell Physiology, vol. 31, no. 6, pp. 859-864.
Nakahori, Kiyoshi ; Koizumi, Kazuji ; Muramatsu, Satoru ; Ohtani, Hiroyuki ; Masuko, Masayuki ; Nakase, Sigeki ; Katou, Kiyoshi ; Okamoto, Hisashi. / Measurement of the respiration-dependent component of intracellular pressure with an improved pressure probe. In: Plant and Cell Physiology. 1990 ; Vol. 31, No. 6. pp. 859-864.
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