Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children

Tomoyuki Akiyama, Mari Akiyama, Yumiko Hayashi, Takashi Shibata, Yoshiyuki Hanaoka, Soichiro Toda, Katsumi Imai, Shin ichiro Hamano, Tohru Okanishi, Harumi Yoshinaga, Katsuhiro Kobayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalClinica Chimica Acta
Volume466
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Pyridoxic Acid
Pyridoxal
Cerebrospinal fluid
Pyridoxal Phosphate
Cerebrospinal Fluid
Epilepsy
Drug therapy
Pediatrics
Linear Models
Drug Therapy
Sex Ratio
High performance liquid chromatography
Linear regression
Regression analysis

Keywords

  • 4-pyridoxic acid
  • Epilepsy
  • Fluorescence detection
  • Liquid chromatography
  • Pyridoxal phosphate
  • Vitamin B6

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children. / Akiyama, Tomoyuki; Akiyama, Mari; Hayashi, Yumiko; Shibata, Takashi; Hanaoka, Yoshiyuki; Toda, Soichiro; Imai, Katsumi; Hamano, Shin ichiro; Okanishi, Tohru; Yoshinaga, Harumi; Kobayashi, Katsuhiro.

In: Clinica Chimica Acta, Vol. 466, 01.03.2017, p. 1-5.

Research output: Contribution to journalArticle

Akiyama, Tomoyuki ; Akiyama, Mari ; Hayashi, Yumiko ; Shibata, Takashi ; Hanaoka, Yoshiyuki ; Toda, Soichiro ; Imai, Katsumi ; Hamano, Shin ichiro ; Okanishi, Tohru ; Yoshinaga, Harumi ; Kobayashi, Katsuhiro. / Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children. In: Clinica Chimica Acta. 2017 ; Vol. 466. pp. 1-5.
@article{6dd398ebc3664671b9f7174d1d3e0ebf,
title = "Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children",
abstract = "Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.",
keywords = "4-pyridoxic acid, Epilepsy, Fluorescence detection, Liquid chromatography, Pyridoxal phosphate, Vitamin B6",
author = "Tomoyuki Akiyama and Mari Akiyama and Yumiko Hayashi and Takashi Shibata and Yoshiyuki Hanaoka and Soichiro Toda and Katsumi Imai and Hamano, {Shin ichiro} and Tohru Okanishi and Harumi Yoshinaga and Katsuhiro Kobayashi",
year = "2017",
month = "3",
day = "1",
doi = "10.1016/j.cca.2016.12.027",
language = "English",
volume = "466",
pages = "1--5",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",

}

TY - JOUR

T1 - Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children

AU - Akiyama, Tomoyuki

AU - Akiyama, Mari

AU - Hayashi, Yumiko

AU - Shibata, Takashi

AU - Hanaoka, Yoshiyuki

AU - Toda, Soichiro

AU - Imai, Katsumi

AU - Hamano, Shin ichiro

AU - Okanishi, Tohru

AU - Yoshinaga, Harumi

AU - Kobayashi, Katsuhiro

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.

AB - Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.

KW - 4-pyridoxic acid

KW - Epilepsy

KW - Fluorescence detection

KW - Liquid chromatography

KW - Pyridoxal phosphate

KW - Vitamin B6

UR - http://www.scopus.com/inward/record.url?scp=85007608266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007608266&partnerID=8YFLogxK

U2 - 10.1016/j.cca.2016.12.027

DO - 10.1016/j.cca.2016.12.027

M3 - Article

C2 - 28040557

AN - SCOPUS:85007608266

VL - 466

SP - 1

EP - 5

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

ER -