TY - JOUR
T1 - Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children
AU - Akiyama, Tomoyuki
AU - Akiyama, Mari
AU - Hayashi, Yumiko
AU - Shibata, Takashi
AU - Hanaoka, Yoshiyuki
AU - Toda, Soichiro
AU - Imai, Katsumi
AU - Hamano, Shin ichiro
AU - Okanishi, Tohru
AU - Yoshinaga, Harumi
AU - Kobayashi, Katsuhiro
N1 - Funding Information:
This study was supported by the Japan Society for the Promotion of Science (Grant Number JP15K09622). This funding source had no involvement in the study design, the collection, analysis, and interpretation of data, the writing of the report, or the decision to submit this article.
Publisher Copyright:
© 2016 Elsevier B.V.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.
AB - Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.
KW - 4-pyridoxic acid
KW - Epilepsy
KW - Fluorescence detection
KW - Liquid chromatography
KW - Pyridoxal phosphate
KW - Vitamin B6
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U2 - 10.1016/j.cca.2016.12.027
DO - 10.1016/j.cca.2016.12.027
M3 - Article
C2 - 28040557
AN - SCOPUS:85007608266
VL - 466
SP - 1
EP - 5
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
ER -