Abstract
Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.
| Original language | English |
|---|---|
| Pages (from-to) | 1-5 |
| Number of pages | 5 |
| Journal | Clinica Chimica Acta |
| Volume | 466 |
| DOIs | |
| Publication status | Published - Mar 1 2017 |
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Keywords
- 4-pyridoxic acid
- Epilepsy
- Fluorescence detection
- Liquid chromatography
- Pyridoxal phosphate
- Vitamin B6
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children. / Akiyama, Tomoyuki; Akiyama, Mari; Hayashi, Yumiko; Shibata, Takashi; Hanaoka, Yoshiyuki; Toda, Soichiro; Imai, Katsumi; Hamano, Shin ichiro; Okanishi, Tohru; Yoshinaga, Harumi; Kobayashi, Katsuhiro.
In: Clinica Chimica Acta, Vol. 466, 01.03.2017, p. 1-5.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children
AU - Akiyama, Tomoyuki
AU - Akiyama, Mari
AU - Hayashi, Yumiko
AU - Shibata, Takashi
AU - Hanaoka, Yoshiyuki
AU - Toda, Soichiro
AU - Imai, Katsumi
AU - Hamano, Shin ichiro
AU - Okanishi, Tohru
AU - Yoshinaga, Harumi
AU - Kobayashi, Katsuhiro
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.
AB - Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers. Methods CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models. Results The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated. Conclusions We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.
KW - 4-pyridoxic acid
KW - Epilepsy
KW - Fluorescence detection
KW - Liquid chromatography
KW - Pyridoxal phosphate
KW - Vitamin B6
UR - http://www.scopus.com/inward/record.url?scp=85007608266&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007608266&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2016.12.027
DO - 10.1016/j.cca.2016.12.027
M3 - Article
C2 - 28040557
AN - SCOPUS:85007608266
VL - 466
SP - 1
EP - 5
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
ER -