Abstract
Interferon-α (IFN-α) was prophylactically administered to the patients with high-stage renal cell cancer after radical nephrectomy. Seventeen patients who were at high risk for recurrence of disease received 500 x 104 units of IFN-α, 2 or 3 times per week for 2 years. Median followup was 21.5 months. Of the patients 4 (23.5%) revealed pulmonary metastasis with or without local recurrence. The administration of IFN-α for 2 patients was discontinued because of side effects. One patient showing pulmonary metastasis has survived, 2 died of cancer and 1 of an unrelated cause. Immunological changes were analyzed by measurement of peripheral blood lymphocyte subsets. Two-color flow cytometry was used to evaluate the lymphocyte subsets consisting of suppressor T lymphocytes (CD8+ CD11b+), cytotoxic T lymphocytes (CD8+ CD11b-), helper T lymphocytes (CD4+ CD45 RA+), suppressor inducer T lymphocytes (CD4+ CD45 RA-), natural killer cells (CD3- CD56+), natural killer T cells (CD3+ CD56+), activated CD8 positive cells (CD8+ HLA-DR+) and activated CD4 positive cells (CD4+ HLA DR+). The number of suppressor T lymphocytes and activated CD8 positive cells increased in the recurrent cases. In contrast, suppressor T lymphocytes gradually decreased after IFN-α treatment, and no evidence of cancer was found in the remaining cases.
| Original language | English |
|---|---|
| Pages (from-to) | 1201-1207 |
| Number of pages | 7 |
| Journal | Biotherapy |
| Volume | 10 |
| Issue number | 9 |
| Publication status | Published - 1996 |
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Keywords
- Adjuvant therapy
- Interferon-α
- Lymphocyte subset
- Renal cell cancer
ASJC Scopus subject areas
- Cancer Research
- Immunology and Allergy
Cite this
Measurement of peripheral blood lymphocyte subsets in patients with renal cell cancer treated with postoperative adjuvant interferon-α therapy. / Miyaji, Y.; Tsushima, T.; Nasu, Yasutomo; Noda, M.; Murakami, T.; Kaku, S.; Ebara, S.; Ohmori, H.
In: Biotherapy, Vol. 10, No. 9, 1996, p. 1201-1207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of peripheral blood lymphocyte subsets in patients with renal cell cancer treated with postoperative adjuvant interferon-α therapy
AU - Miyaji, Y.
AU - Tsushima, T.
AU - Nasu, Yasutomo
AU - Noda, M.
AU - Murakami, T.
AU - Kaku, S.
AU - Ebara, S.
AU - Ohmori, H.
PY - 1996
Y1 - 1996
N2 - Interferon-α (IFN-α) was prophylactically administered to the patients with high-stage renal cell cancer after radical nephrectomy. Seventeen patients who were at high risk for recurrence of disease received 500 x 104 units of IFN-α, 2 or 3 times per week for 2 years. Median followup was 21.5 months. Of the patients 4 (23.5%) revealed pulmonary metastasis with or without local recurrence. The administration of IFN-α for 2 patients was discontinued because of side effects. One patient showing pulmonary metastasis has survived, 2 died of cancer and 1 of an unrelated cause. Immunological changes were analyzed by measurement of peripheral blood lymphocyte subsets. Two-color flow cytometry was used to evaluate the lymphocyte subsets consisting of suppressor T lymphocytes (CD8+ CD11b+), cytotoxic T lymphocytes (CD8+ CD11b-), helper T lymphocytes (CD4+ CD45 RA+), suppressor inducer T lymphocytes (CD4+ CD45 RA-), natural killer cells (CD3- CD56+), natural killer T cells (CD3+ CD56+), activated CD8 positive cells (CD8+ HLA-DR+) and activated CD4 positive cells (CD4+ HLA DR+). The number of suppressor T lymphocytes and activated CD8 positive cells increased in the recurrent cases. In contrast, suppressor T lymphocytes gradually decreased after IFN-α treatment, and no evidence of cancer was found in the remaining cases.
AB - Interferon-α (IFN-α) was prophylactically administered to the patients with high-stage renal cell cancer after radical nephrectomy. Seventeen patients who were at high risk for recurrence of disease received 500 x 104 units of IFN-α, 2 or 3 times per week for 2 years. Median followup was 21.5 months. Of the patients 4 (23.5%) revealed pulmonary metastasis with or without local recurrence. The administration of IFN-α for 2 patients was discontinued because of side effects. One patient showing pulmonary metastasis has survived, 2 died of cancer and 1 of an unrelated cause. Immunological changes were analyzed by measurement of peripheral blood lymphocyte subsets. Two-color flow cytometry was used to evaluate the lymphocyte subsets consisting of suppressor T lymphocytes (CD8+ CD11b+), cytotoxic T lymphocytes (CD8+ CD11b-), helper T lymphocytes (CD4+ CD45 RA+), suppressor inducer T lymphocytes (CD4+ CD45 RA-), natural killer cells (CD3- CD56+), natural killer T cells (CD3+ CD56+), activated CD8 positive cells (CD8+ HLA-DR+) and activated CD4 positive cells (CD4+ HLA DR+). The number of suppressor T lymphocytes and activated CD8 positive cells increased in the recurrent cases. In contrast, suppressor T lymphocytes gradually decreased after IFN-α treatment, and no evidence of cancer was found in the remaining cases.
KW - Adjuvant therapy
KW - Interferon-α
KW - Lymphocyte subset
KW - Renal cell cancer
UR - http://www.scopus.com/inward/record.url?scp=0029839859&partnerID=8YFLogxK
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M3 - Article
AN - SCOPUS:0029839859
VL - 10
SP - 1201
EP - 1207
JO - Biotherapy
JF - Biotherapy
SN - 0914-2223
IS - 9
ER -