Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension

Masashi Tachikawa, Nobuhiro Morone, Yosuke Senju, Tadao Sugiura, Kyoko Hanawa-Suetsugu, Atsushi Mochizuki, Shiro Suetsugu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates.

Original languageEnglish
Article number7794
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017
Externally publishedYes

Fingerprint

Caveolin 1
Caveolae
Cell Membrane
Membranes
Detergents
Caveolins
Buffers

ASJC Scopus subject areas

  • General

Cite this

Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension. / Tachikawa, Masashi; Morone, Nobuhiro; Senju, Yosuke; Sugiura, Tadao; Hanawa-Suetsugu, Kyoko; Mochizuki, Atsushi; Suetsugu, Shiro.

In: Scientific reports, Vol. 7, No. 1, 7794, 01.12.2017.

Research output: Contribution to journalArticle

Tachikawa, Masashi ; Morone, Nobuhiro ; Senju, Yosuke ; Sugiura, Tadao ; Hanawa-Suetsugu, Kyoko ; Mochizuki, Atsushi ; Suetsugu, Shiro. / Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension. In: Scientific reports. 2017 ; Vol. 7, No. 1.
@article{03f36b33b2464ed4a2ee5c4e230167b1,
title = "Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension",
abstract = "Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates.",
author = "Masashi Tachikawa and Nobuhiro Morone and Yosuke Senju and Tadao Sugiura and Kyoko Hanawa-Suetsugu and Atsushi Mochizuki and Shiro Suetsugu",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-08259-5",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension

AU - Tachikawa, Masashi

AU - Morone, Nobuhiro

AU - Senju, Yosuke

AU - Sugiura, Tadao

AU - Hanawa-Suetsugu, Kyoko

AU - Mochizuki, Atsushi

AU - Suetsugu, Shiro

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates.

AB - Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates.

UR - http://www.scopus.com/inward/record.url?scp=85046889838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046889838&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-08259-5

DO - 10.1038/s41598-017-08259-5

M3 - Article

C2 - 28798329

AN - SCOPUS:85046889838

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 7794

ER -