MCI-186 prevents spinal cord damage and affects enzyme levels of nitric oxide synthase and Cu/Zn superoxide dismutase after transient ischemia in rabbits

Goro Takahashi, Masahiro Sakurai, Koji Abe, Yasuto Itoyama, Koichi Tabayashi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective: The mechanism of spinal cord injury is believed to be related to the vulnerability of spinal motor neuron cells against ischemia. We tested whether MCI-186, which is useful for treating ischemic damage in the brain, can protect against ischemic spinal cord damage. Methods: After induction of ischemia, MCI-186 or vehicle was injected intravenously. Cell damage was analyzed by observing the function of the lower limbs and by counting the number of motor neurons. To investigate the mechanism by which MCI-186 prevents ischemic spinal cord damage, we observed the immunoreactivity of Cu/Zn superoxide dismutase, neuronal nitric oxide synthase, and endothelial nitric oxide synthase. Results: MCI-186 eased the functional deficits and increased the number of motor neurons after ischemia. The induction of neuronal nitric oxide synthase was significantly reduced by the treatment with MCI-186. Furthermore, the increase in the induction of endothelial nitric oxide synthase and Cu/Zn superoxide dismutase was more pronounced. Conclusion: These results indicate that MCI-186 may protect motor neurons from ischemic injury by reducing neuronal nitric oxide synthase and increasing endothelial nitric oxide synthase. MCI-186 may be a strong candidate for use as a therapeutic agent in the treatment of ischemic spinal cord injury.

Original languageEnglish
Pages (from-to)1461-1466
Number of pages6
JournalJournal of Thoracic and Cardiovascular Surgery
Volume126
Issue number5
DOIs
Publication statusPublished - Nov 2003

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Nitric Oxide Synthase
Spinal Cord
Ischemia
Rabbits
Motor Neurons
Nitric Oxide Synthase Type I
Enzymes
Nitric Oxide Synthase Type III
Spinal Cord Injuries
Superoxide Dismutase-1
phenylmethylpyrazolone
Lower Extremity
Wounds and Injuries
Brain

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

MCI-186 prevents spinal cord damage and affects enzyme levels of nitric oxide synthase and Cu/Zn superoxide dismutase after transient ischemia in rabbits. / Takahashi, Goro; Sakurai, Masahiro; Abe, Koji; Itoyama, Yasuto; Tabayashi, Koichi.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 126, No. 5, 11.2003, p. 1461-1466.

Research output: Contribution to journalArticle

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AU - Tabayashi, Koichi

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