Maturation pathway of metalloprotease produced by Aeromonas sobria

Eizo Takahashi, Yoshio Fujii, Hidetomo Kobayashi, Hiroyasu Yamanaka, Gopinath Balakrish Nair, Yoshifumi Takeda, Sakae Arimoto, Tomoe Negishi, Keinosuke Okamoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Previously, we cloned the metalloprotease gene of Aeromonas sobria (amp) and determined its nucleotide sequence (GenBank accession number DQ784565). The protease is composed of 591 amino acid residues. In this study, we purified the mature metalloprotease fromthe culture supernatant of A. sobria and determined the amino terminal sequence and molecular size ofAMP. In addition,we examined the production of AMP diachronically and found that AMP emerges outside of the cell as an intermediate composed of mature and propeptide regions. Subsequently, we determined that the N-terminal amino acid sequence of the intermediate and found that the sequence is identical to that of the mature metalloprotease. This means that the intermediate is composed of a matureAMPregion and aC-terminal propeptide. The cross culture experiment of mutants of metalloprotease and serine protease of A. sobria on skim milk agar medium indicates that the intermediate released outside of the cell is inactive and that serine protease produced by A. sobria accelerates the conversion of the intermediate fromthe inactive to the active form.

Original languageEnglish
Pages (from-to)596-605
Number of pages10
JournalMICROBIOLOGY and IMMUNOLOGY
Volume54
Issue number10
DOIs
Publication statusPublished - Oct 2010

Keywords

  • Aeromonas sobria
  • Metalloprotease

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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    Takahashi, E., Fujii, Y., Kobayashi, H., Yamanaka, H., Nair, G. B., Takeda, Y., Arimoto, S., Negishi, T., & Okamoto, K. (2010). Maturation pathway of metalloprotease produced by Aeromonas sobria. MICROBIOLOGY and IMMUNOLOGY, 54(10), 596-605. https://doi.org/10.1111/j.1348-0421.2010.00258.x