Abstract
It is widely accepted that fibrosis is frequently observed in the gingiva of smokers. However, the mechanisms by which smoking results in pathological changes in periodontal tissue that lead to fibrosis are not entirely clear. Our former report showed that type I collagen synthesis was promoted by nicotine via CCN family protein 2 in human periodontal tissue cells. Here, we evaluated other aspects of nicotine function from a viewpoint of extracellular matrix (ECM) remodeling. Human gingival fibroblasts (n = 4) and periodontal ligament cells (n = 3) were isolated. The cells were treated with nicotine at a variety of concentrations for 12–48 h. Modulators of matrix remodeling were measured using enzyme-linked immunosorbent assays. Cell migration and morphology were also evaluated. As a result, following treatment with 1 μg/ml nicotine, tissue inhibitor of metalloproteinase-1 and transforming growth factor-β1 production in both cell lysates and supernatants, and matrix metalloproteinases-1 production in cell lysates, were significantly increased (p
| Original language | English |
|---|---|
| Pages (from-to) | 35-43 |
| Number of pages | 9 |
| Journal | Odontology |
| Volume | 104 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2016 |
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Keywords
- Fibrosis
- Gingival fibrosis
- Matrix remodeling
- Nicotine
- Smoking
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Matrix remodeling response of human periodontal tissue cells toward fibrosis upon nicotine exposure. / Takeuchi-Igarashi, Hiroko; Kubota, Satoshi; Tachibana, Toshiaki; Murakashi, Etsuko; Takigawa, Masaharu; Okabe, Masataka; Numabe, Yukihiro.
In: Odontology, Vol. 104, No. 1, 01.01.2016, p. 35-43.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Matrix remodeling response of human periodontal tissue cells toward fibrosis upon nicotine exposure
AU - Takeuchi-Igarashi, Hiroko
AU - Kubota, Satoshi
AU - Tachibana, Toshiaki
AU - Murakashi, Etsuko
AU - Takigawa, Masaharu
AU - Okabe, Masataka
AU - Numabe, Yukihiro
PY - 2016/1/1
Y1 - 2016/1/1
N2 - It is widely accepted that fibrosis is frequently observed in the gingiva of smokers. However, the mechanisms by which smoking results in pathological changes in periodontal tissue that lead to fibrosis are not entirely clear. Our former report showed that type I collagen synthesis was promoted by nicotine via CCN family protein 2 in human periodontal tissue cells. Here, we evaluated other aspects of nicotine function from a viewpoint of extracellular matrix (ECM) remodeling. Human gingival fibroblasts (n = 4) and periodontal ligament cells (n = 3) were isolated. The cells were treated with nicotine at a variety of concentrations for 12–48 h. Modulators of matrix remodeling were measured using enzyme-linked immunosorbent assays. Cell migration and morphology were also evaluated. As a result, following treatment with 1 μg/ml nicotine, tissue inhibitor of metalloproteinase-1 and transforming growth factor-β1 production in both cell lysates and supernatants, and matrix metalloproteinases-1 production in cell lysates, were significantly increased (p
AB - It is widely accepted that fibrosis is frequently observed in the gingiva of smokers. However, the mechanisms by which smoking results in pathological changes in periodontal tissue that lead to fibrosis are not entirely clear. Our former report showed that type I collagen synthesis was promoted by nicotine via CCN family protein 2 in human periodontal tissue cells. Here, we evaluated other aspects of nicotine function from a viewpoint of extracellular matrix (ECM) remodeling. Human gingival fibroblasts (n = 4) and periodontal ligament cells (n = 3) were isolated. The cells were treated with nicotine at a variety of concentrations for 12–48 h. Modulators of matrix remodeling were measured using enzyme-linked immunosorbent assays. Cell migration and morphology were also evaluated. As a result, following treatment with 1 μg/ml nicotine, tissue inhibitor of metalloproteinase-1 and transforming growth factor-β1 production in both cell lysates and supernatants, and matrix metalloproteinases-1 production in cell lysates, were significantly increased (p
KW - Fibrosis
KW - Gingival fibrosis
KW - Matrix remodeling
KW - Nicotine
KW - Smoking
UR - http://www.scopus.com/inward/record.url?scp=84953254305&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84953254305&partnerID=8YFLogxK
U2 - 10.1007/s10266-014-0177-y
DO - 10.1007/s10266-014-0177-y
M3 - Article
C2 - 25316032
AN - SCOPUS:84953254305
VL - 104
SP - 35
EP - 43
JO - Odontology / the Society of the Nippon Dental University
JF - Odontology / the Society of the Nippon Dental University
SN - 1618-1247
IS - 1
ER -