Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro

Megumi Kawagoe, Eichi Tsuruga, Kyoko Oka, Yoshihiko Sawa, Hiroyuki Ishikawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Oxytalan fibers are distributed in the eye and periodontal ligaments (PDL). The ciliary zonule, known as Zinn's zonule, in the eye is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1 and fibrillin-2. As turnover of oxytalan fibers is slow during life, their degradation mechanism remains unclarified. This study was performed to examine degradation pattern of fibrillin-1 and fibrillin-2 by experimental MMP activation. We cultured human non-pigmented ciliary epithelial cells (HNPCEC) and PDL fibroblasts for 7 days, then treated them with concanavalin A to activate matrix metalloproteinase (MMP)-2, and examined the degradation of fibrillin-1 and fibrillin-2 for 72 hr using immunofluorescence. At 7 days of HNPCEC culture, fibrillin-1-positive fibers were observed, some of which merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin and disappeared by 72 hr, while fibrillin-2-positive fibers disappeared almost completely within 24 hr. At 7 days of PDL fibroblast culture, fibrillin-1-positive fibers were mostly merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin by 24 hr and had almost disappeared by 48 hr, while fibrillin-2-positive fibers decreased constantly after 24 hr. A MMP-2 inhibitor completely suppressed these degradations. These results suggest that the patterns of fibrillin-1 and fibrillin-2 degradation differ between the eye and the PDL, possibly reflecting the sensitivity of fibrillin-1 and fibrillin-2 of each type of oxytalan fiber against MMP-2.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalActa Histochemica et Cytochemica
Volume46
Issue number5
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Periodontal Ligament
Matrix Metalloproteinase 2
Ligaments
Fibers
Degradation
Chemical activation
Fibroblasts
Cell culture
Epithelial Cells
Microfibrils
oxytalan
Fibrillin-1
In Vitro Techniques
Fibrillin-2
Matrix Metalloproteinase Inhibitors
Concanavalin A
Matrix Metalloproteinases
Corrosion inhibitors
Fluorescent Antibody Technique
Cell Culture Techniques

Keywords

  • Ciliary zonule
  • Fibrillin
  • Microfibrils
  • Oxytalan fibers

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Pathology and Forensic Medicine
  • Histology

Cite this

Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro. / Kawagoe, Megumi; Tsuruga, Eichi; Oka, Kyoko; Sawa, Yoshihiko; Ishikawa, Hiroyuki.

In: Acta Histochemica et Cytochemica, Vol. 46, No. 5, 2013, p. 153-159.

Research output: Contribution to journalArticle

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AU - Oka, Kyoko

AU - Sawa, Yoshihiko

AU - Ishikawa, Hiroyuki

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AB - Oxytalan fibers are distributed in the eye and periodontal ligaments (PDL). The ciliary zonule, known as Zinn's zonule, in the eye is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1 and fibrillin-2. As turnover of oxytalan fibers is slow during life, their degradation mechanism remains unclarified. This study was performed to examine degradation pattern of fibrillin-1 and fibrillin-2 by experimental MMP activation. We cultured human non-pigmented ciliary epithelial cells (HNPCEC) and PDL fibroblasts for 7 days, then treated them with concanavalin A to activate matrix metalloproteinase (MMP)-2, and examined the degradation of fibrillin-1 and fibrillin-2 for 72 hr using immunofluorescence. At 7 days of HNPCEC culture, fibrillin-1-positive fibers were observed, some of which merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin and disappeared by 72 hr, while fibrillin-2-positive fibers disappeared almost completely within 24 hr. At 7 days of PDL fibroblast culture, fibrillin-1-positive fibers were mostly merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin by 24 hr and had almost disappeared by 48 hr, while fibrillin-2-positive fibers decreased constantly after 24 hr. A MMP-2 inhibitor completely suppressed these degradations. These results suggest that the patterns of fibrillin-1 and fibrillin-2 degradation differ between the eye and the PDL, possibly reflecting the sensitivity of fibrillin-1 and fibrillin-2 of each type of oxytalan fiber against MMP-2.

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