Mast cells modulate interleukin-4 production independently of interferon-gamma generation by invariant Natural Killer T cells through the suppression of Notch Signaling

Daisuke Kurihara, Nobuyuki Fukuishi, Kazue Kadota, Hirofumi Hamano, Hayato Teruya, Nobuaki Matsui, Masaaki Akagi

Research output: Contribution to journalArticlepeer-review

Abstract

The spatial contact of immune cells is predominant machinery for the modulation of local inflammatory sites as much as humoral regulation is for inflammation. In this study, we investigated the role of mast cells in the regulation of invariant natural killer T cells (iNKTs) functions. Co-cultivation of bonemarrow derived mouse mast cells (BMMCs) with purified and stimulated mouse iNKTs caused marked suppression of interleukin (IL)-4 but not interferon (IFN)-γ production in iNKTs. Suppression was accompanied by down-regulation of IL-4 mRNA expression. The Notch intracellular cytoplasmic domain in iNKTs was also completely diminished by co-cultivation with BMMCs. Suppressed IL-4 production was recovered when iNKTs were separately co-cultured with BMMCs using the transwell system. Furthermore, co-culture of iNKTs with activated BMMCs elicited partial suppression of IL-4 production. These findings suggest that mast cells modulate the function of iNKTs via the inhibition of Notch signaling.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalJournal of Health Science
Volume57
Issue number2
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

Keywords

  • Cell-cell contact
  • Invariant natural killer T cell
  • Mast cell
  • Notch signaling

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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