TY - JOUR
T1 - Mass spectrometric map of neuropeptide expression and analysis of the γ-prepro-tachykinin gene expression in the medaka (Oryzias latipes) brain
AU - Suehiro, Yuji
AU - Yasuda, Akikazu
AU - Okuyama, Teruhiro
AU - Imada, Haruka
AU - Kuroyanagi, Yoshiko
AU - Kubo, Takeo
AU - Takeuchi, Hideaki
N1 - Funding Information:
We thank Dr. Atsuko Shimada, Profs. Hiroyuki Takeda (The University of Tokyo) and Kiyoshi Naruse (National Institute for Basic Biology) for discussion and for instructing us in the handling of medaka fish. We thank the Medaka National BioResource Project for the use of the medaka cDNA database. This work was supported by the Ministry of Education, Culture, Sports, Science, and Technology, Scientific Research on Priority Areas (Area No. 454, Mobiligence Project).
PY - 2009/3
Y1 - 2009/3
N2 - Neuropeptides have important roles in modulating behavioral patterns such as social interaction. With the aim to determine the presence of neuropeptides known to be involved in social interaction as well as novel peptides, we used MALDI-TOF/MS to analyze neuropeptide profiles in some medaka brain regions. In the telencephalon, hypothalamus, and pituitary gland, 3, 6, and 10 peaks, respectively, were identified as neuropeptides (Arg-vasotocin [AVT], growth hormone-releasing hormone [GHRH], neuropeptide FF, substance P [SP], somatostatin-1 and -2, melanin-concentrating hormone [MCH], MCH gene-related peptide [Mgrp], melanocyte-stimulating hormone [MSH], corticotropin-like intermediate lobe peptide [CLIP], and β-endorphin). The neuropeptide profile of telencephalon similar to that of the hypothalamus, but completely different from that of pituitary gland. For the future genetic analysis, we identified cDNAs encoding precursor proteins for the identified peptides. We also detect its expression of γ-prepro-tachykinin gene encoding a SP precursor protein in both the telencephalon and hypothalamus. Our results indicated that the medaka brain contains some neuropeptides (AVT, SP, and somatostatins) that may be involved in modulating medaka behaviors such as social interaction.
AB - Neuropeptides have important roles in modulating behavioral patterns such as social interaction. With the aim to determine the presence of neuropeptides known to be involved in social interaction as well as novel peptides, we used MALDI-TOF/MS to analyze neuropeptide profiles in some medaka brain regions. In the telencephalon, hypothalamus, and pituitary gland, 3, 6, and 10 peaks, respectively, were identified as neuropeptides (Arg-vasotocin [AVT], growth hormone-releasing hormone [GHRH], neuropeptide FF, substance P [SP], somatostatin-1 and -2, melanin-concentrating hormone [MCH], MCH gene-related peptide [Mgrp], melanocyte-stimulating hormone [MSH], corticotropin-like intermediate lobe peptide [CLIP], and β-endorphin). The neuropeptide profile of telencephalon similar to that of the hypothalamus, but completely different from that of pituitary gland. For the future genetic analysis, we identified cDNAs encoding precursor proteins for the identified peptides. We also detect its expression of γ-prepro-tachykinin gene encoding a SP precursor protein in both the telencephalon and hypothalamus. Our results indicated that the medaka brain contains some neuropeptides (AVT, SP, and somatostatins) that may be involved in modulating medaka behaviors such as social interaction.
KW - Medaka
KW - Social interaction
KW - Somatostatin
KW - Tachykinin
KW - Vasotocin
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U2 - 10.1016/j.ygcen.2008.12.001
DO - 10.1016/j.ygcen.2008.12.001
M3 - Article
C2 - 19118555
AN - SCOPUS:60449088072
VL - 161
SP - 138
EP - 145
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
SN - 0016-6480
IS - 1
ER -