Mass spectrometric map of neuropeptide expression and analysis of the γ-prepro-tachykinin gene expression in the medaka (Oryzias latipes) brain

Yuji Suehiro, Akikazu Yasuda, Teruhiro Okuyama, Haruka Imada, Yoshiko Kuroyanagi, Takeo Kubo, Hideaki Takeuchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Neuropeptides have important roles in modulating behavioral patterns such as social interaction. With the aim to determine the presence of neuropeptides known to be involved in social interaction as well as novel peptides, we used MALDI-TOF/MS to analyze neuropeptide profiles in some medaka brain regions. In the telencephalon, hypothalamus, and pituitary gland, 3, 6, and 10 peaks, respectively, were identified as neuropeptides (Arg-vasotocin [AVT], growth hormone-releasing hormone [GHRH], neuropeptide FF, substance P [SP], somatostatin-1 and -2, melanin-concentrating hormone [MCH], MCH gene-related peptide [Mgrp], melanocyte-stimulating hormone [MSH], corticotropin-like intermediate lobe peptide [CLIP], and β-endorphin). The neuropeptide profile of telencephalon similar to that of the hypothalamus, but completely different from that of pituitary gland. For the future genetic analysis, we identified cDNAs encoding precursor proteins for the identified peptides. We also detect its expression of γ-prepro-tachykinin gene encoding a SP precursor protein in both the telencephalon and hypothalamus. Our results indicated that the medaka brain contains some neuropeptides (AVT, SP, and somatostatins) that may be involved in modulating medaka behaviors such as social interaction.

Original languageEnglish
Pages (from-to)138-145
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume161
Issue number1
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Keywords

  • Medaka
  • Social interaction
  • Somatostatin
  • Tachykinin
  • Vasotocin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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