Mass fabrication of small cell spheroids by using micro-patterned tissue culture plate

Akinari Iwasaki, Takuya Matsumoto, Go Tazaki, Hitoshi Tsuruta, Hiroshi Egusa, Hiroyuki Miyajima, Taiji Sohmura

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A newly designed micro-patterned chamber was utilized to fabricate cell spheroids with a constant size (<200μm) and cell number. By applying cytochalasin D as a chemical to control cell adhesion and aggregation, thousands of aggregated cells were formed in each patterned chamber. Importantly, the formed cell spheroids were collected by a simple pipetting process without using proteinase.

Original languageEnglish
Pages (from-to)801-804
Number of pages4
JournalAdvanced Engineering Materials
Volume11
Issue number10
DOIs
Publication statusPublished - Oct 2009
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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