Marine acidophilic sulfur-oxidizing bacterium requiring salts for the oxidation of reduced inorganic sulfur compounds

Kazuo Kamimura, Emi Higashino, Souichi Moriya, Tsuyoshi Sugio

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An acidophilic sulfur-oxidizing bacterium was isolated from seawater, and designated as strain SH. Strain SH was a Gram-negative, rod-shaped and motile bacterium, which had an optimum temperature and pH value for growth of 30°C and 4.0, respectively. The mol% guanine plus cytosine of the DNA was 46.0. Chemolithotrophic growth was observed with elemental sulfur and tetrathionate at pH 4.0, and was not observed with ferrous ion. The isolate was able to utilize carbon dioxide as a carbon source, and was unable to grow heterotrophically with yeast extract or glucose. The growth of strain SH was activated in medium supplemented with NaCl. However, LiCl and KCl did not sustain the growth of strain SH. The results indicate that strain SH was an acidophilic, halophilic, and obligately chemolithotrophic sulfur-oxidizing bacterium. Phylogenetic analysis based on 16S rDNA sequences indicated that strain SH had a close relationship to Acidithiobacillus thiooxidans. The oxidizing activities of sulfur and sulfite with resting cells were stimulated not only by the addition of NaCl, but also by KCl and LiCl. The oxidation of sulfite was inhibited by ionophores, carbonyl cyanide-m-chlorophenylhydrazone (CCCP), and monensin, and respiratory inhibitors, KCN and 2-heptyl-4-hydroxy-quinoline-N-oxode (HQNO).

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalExtremophiles
Volume7
Issue number2
DOIs
Publication statusPublished - Apr 2003

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Sulfur Compounds
Sulfur
Salts
Bacteria
Sulfites
Chemoautotrophic Growth
Acidithiobacillus thiooxidans
Growth
Monensin
Cytosine
Ionophores
Guanine
Seawater
Ribosomal DNA
Carbon Dioxide
Carbon
Yeasts
Ions
Glucose
Temperature

Keywords

  • Acidithiobacillus
  • Acidophile
  • Chemolithotroph
  • Halophile
  • Phylogeny
  • Sulfur-oxidizing bacterium

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Marine acidophilic sulfur-oxidizing bacterium requiring salts for the oxidation of reduced inorganic sulfur compounds. / Kamimura, Kazuo; Higashino, Emi; Moriya, Souichi; Sugio, Tsuyoshi.

In: Extremophiles, Vol. 7, No. 2, 04.2003, p. 95-99.

Research output: Contribution to journalArticle

Kamimura, Kazuo ; Higashino, Emi ; Moriya, Souichi ; Sugio, Tsuyoshi. / Marine acidophilic sulfur-oxidizing bacterium requiring salts for the oxidation of reduced inorganic sulfur compounds. In: Extremophiles. 2003 ; Vol. 7, No. 2. pp. 95-99.
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