Mapping of cDNA clones on contig of Chlorella chromosome I

Sinya Maki, Yutaka Ohta, Yoshiteru Noutoshi, Makoto Fujie, Shohji Usami, Takashi Yamada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Complementary DNA (cDNA) clones specific to the smallest chromosome (chromosome I) of Chlorella vulgaris C-169 were selected from cDNA libraries with probes of chromosome I DNA fragments amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). A total of 15 clones was obtained, which included gene homologs for α-tubulin, inosine-5'-monophosphate dehydrogenase, β-1,4-mannase, a TTG-binding protein, a heat shock protein, thioredoxin/protein disulfide isomerase, transcription factor NF-E2, an oxidoreductase, and UDP-n-acetylglucosamine enolpyruvyltransferase. These clones were definitely localized at specific sites on the chromosome I physical map constructed on the basis of overlapping cosmid clones (the contig). They were predominantly distributed within the left two-thirds of the chromosome. This contrasts with the distribution of repetitive elements such as short interspersed elements (SINEs), which are rather abundant in the right two-thirds of chromosome I. The comparative simplicity of the gene arrangement of Chlorella chromosome I suggests that it may be able to serve as a prototypic system for deciphering the complexity of huge plant chromosomes.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume90
Issue number4
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

Keywords

  • Chlorella chromosome I
  • Cosmid contig
  • DOP-PCR
  • Streptavidin-biotin affinity
  • cDNA mapping

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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