Mapping of a hypovirus p29 protease symptom determinant domain with sequence similarity to potyvirus HC-Pro protease

Nobuhiro Suzuki, Baoshan Chen, Donald L. Nuss

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Hypovirus infection of the chestnut blight fungus Cryphonectria parasitica results in a spectrum of phenotypic changes that can include alterations in colony morphology and significant reductions in pigmentation, asexual sporulation, and virulence (hypovirulence). Deletion of 88% [Phe(25) to Pro(243)] of the virus-encoded papain-like protease, p29, in the context of an infectious cDNA clone of the prototypic hypovirus CHV1-EP713 (recombinant virus Ap29) partially relieved virus-mediated suppression of pigmentation and sporulation without altering the level of hypovirulence. We now report mapping of the p29 symptom determinant domain to a region extending from Phe(25) through Gln(73) by a gain-of-function analysis following progressive repair of the Δp29 deletion mutant. This domain was previously shown to share sequence similarity [including conserved cysteine residues Cys(38), Cys(48), Cys(70), and Cys(72)] with the N-terminal portion of the potyvirus-encoded helper component-proteinase (HC-Pro), a multifunctional protein implicated in aphid-mediated transmission, genome amplification, polyprotein processing, long-distance movement, and suppression of posttranscriptional silencing. Substitution of a glycine residue for either Cys(38) or Cys(48) resulted in no qualitative or quantitative changes in virus-mediated symptoms. Unexpectedly, mutation of Cys(70) resulted in a very severe phenotype that included significantly reduced mycelial growth and profoundly altered colony morphology. In contrast, substitution for Cys(72) resulted in a less severe symptom phenotype approaching that observed for Δp29. The finding that p29-mediated symptom expression is influenced by two cysteine residues that are conserved in the potyvirus-encoded HC-Pro raises the possibility that these related viral-papain-like proteases function in their respective fungal and plant hosts by impacting ancestrally related regulatory pathways.

Original languageEnglish
Pages (from-to)9478-9484
Number of pages7
JournalJournal of Virology
Volume73
Issue number11
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Hypovirus
Potyvirus
signs and symptoms (plants)
Peptide Hydrolases
proteinases
Viruses
Cryphonectria parasitica
viruses
virulence
Papain
papain
Pigmentation
sporulation
pigmentation
Cysteine
cysteine
Phenotype
phenotype
Polyproteins
Aphids

ASJC Scopus subject areas

  • Immunology

Cite this

Mapping of a hypovirus p29 protease symptom determinant domain with sequence similarity to potyvirus HC-Pro protease. / Suzuki, Nobuhiro; Chen, Baoshan; Nuss, Donald L.

In: Journal of Virology, Vol. 73, No. 11, 1999, p. 9478-9484.

Research output: Contribution to journalArticle

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