Mapping by transposons of the inversion termini in Escherichia coli K-12 strain 1485IN

M. Enomoto, Y. Komoda, A. Tominaga

Research output: Contribution to journalArticle

Abstract

Strain 1485IN carries a chromosomal inversion which corresponds to 35% of the chromosome and includes proC, trp and his genes. The termini of the inversion lie between the lac and proC loci and between his and cdd of the normal strain. Using Tn10 and Tn5 in transduction crosses between the normal and inversion strains, the termini were mapped to sites located approximately 0.25 min and 1.6 min away from proC and his, respectively within a region of roughly 4 kb long. The crosses where the normal strains carrying Tn10 near the terminus are donors and the inversion strain is a recipient, yielded unusual Tet(r) His- recombinants, which arose from illegitimate recombination leading to the replacement of a chromosomal his+ region with a transducing fragment carrying proC. Another rearrangement was detected between the normal and inversion strains in a region outside the inverted segment near the cdd locus.

Original languageEnglish
Pages (from-to)631-638
Number of pages8
JournalGenetics
Volume129
Issue number3
Publication statusPublished - Jan 1 1991

ASJC Scopus subject areas

  • Genetics

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