TY - JOUR
T1 - MAP3Kinase-dependent SnRK2-kinase activation is required for abscisic acid signal transduction and rapid osmotic stress response
AU - Takahashi, Yohei
AU - Zhang, Jingbo
AU - Hsu, Po Kai
AU - Ceciliato, Paulo H.O.
AU - Zhang, Li
AU - Dubeaux, Guillaume
AU - Munemasa, Shintaro
AU - Ge, Chennan
AU - Zhao, Yunde
AU - Hauser, Felix
AU - Schroeder, Julian I.
N1 - Funding Information:
This research was funded by National Institutes of Health (GM060396-ES010337) and National Science Foundation (MCB-1900567) grants to J.I.S., a Postdoctral Fellowship for Research Abroad from the Japan Society for the promotion of Science to Y.T., a Ciencias sem Fronteiras/CNPq fellowship (203406/2014-1) to P.H.O.C. and a scholarship from the China Scholarship Council to L.Z., an EMBO long-term post-doctoral fellowship (ALTF334-2018) to G.D., and in part NIH grant GM114660 to Y.Z. We thank Dr. Majid Ghassemian (University of California, San Diego) for advice at analyzing LC-MS/ MS data (National Institutes of Health grant number: S10OD021724). We thank Mr.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Abiotic stresses, including drought and salinity, trigger a complex osmotic-stress and abscisic acid (ABA) signal transduction network. The core ABA signalling components are snf1-related protein kinase2s (SnRK2s), which are activated by ABA-triggered inhibition of type-2C protein-phosphatases (PP2Cs). SnRK2 kinases are also activated by a rapid, largely unknown, ABA-independent osmotic-stress signalling pathway. Here, through a combination of a redundancy-circumventing genetic screen and biochemical analyses, we have identified functionally-redundant MAPKK-kinases (M3Ks) that are necessary for activation of SnRK2 kinases. These M3Ks phosphorylate a specific SnRK2/OST1 site, which is indispensable for ABA-induced reactivation of PP2C-dephosphorylated SnRK2 kinases. ABA-triggered SnRK2 activation, transcription factor phosphorylation and SLAC1 activation require these M3Ks in vitro and in plants. M3K triple knock-out plants show reduced ABA sensitivity and strongly impaired rapid osmotic-stress-induced SnRK2 activation. These findings demonstrate that this M3K clade is required for ABA- and osmotic-stress-activation of SnRK2 kinases, enabling robust ABA and osmotic stress signal transduction.
AB - Abiotic stresses, including drought and salinity, trigger a complex osmotic-stress and abscisic acid (ABA) signal transduction network. The core ABA signalling components are snf1-related protein kinase2s (SnRK2s), which are activated by ABA-triggered inhibition of type-2C protein-phosphatases (PP2Cs). SnRK2 kinases are also activated by a rapid, largely unknown, ABA-independent osmotic-stress signalling pathway. Here, through a combination of a redundancy-circumventing genetic screen and biochemical analyses, we have identified functionally-redundant MAPKK-kinases (M3Ks) that are necessary for activation of SnRK2 kinases. These M3Ks phosphorylate a specific SnRK2/OST1 site, which is indispensable for ABA-induced reactivation of PP2C-dephosphorylated SnRK2 kinases. ABA-triggered SnRK2 activation, transcription factor phosphorylation and SLAC1 activation require these M3Ks in vitro and in plants. M3K triple knock-out plants show reduced ABA sensitivity and strongly impaired rapid osmotic-stress-induced SnRK2 activation. These findings demonstrate that this M3K clade is required for ABA- and osmotic-stress-activation of SnRK2 kinases, enabling robust ABA and osmotic stress signal transduction.
UR - http://www.scopus.com/inward/record.url?scp=85077457173&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077457173&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-13875-y
DO - 10.1038/s41467-019-13875-y
M3 - Article
C2 - 31896774
AN - SCOPUS:85077457173
VL - 11
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 12
ER -