Mannose receptor, C type 1 contributes to bacterial uptake by placental trophoblast giant cells

Masanori Hashino, Masato Tachibana, Takashi Shimizu, Masahisa Watarai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

During pregnancy, maternal immune function is strictly controlled and immune tolerance is induced. Trophoblast giant (TG) cells exhibit phagocytic activity and show macrophage-like activity against microorganisms in the placenta. However, details of molecular receptors and mechanisms for uptake by TG cells have not been clarified. In this study, we investigated the involvement of the mannose receptor, C type 1 (MRC1), in the uptake of the abortion-inducible bacterium Listeria monocytogenes and abortion-uninducible bacteria Bacillus subtilis and Escherichia coli by TG cells differentiated from a mouse trophoblast stem cell line in vitro. Knockdown of MRC1 inhibited the uptake of all of these bacteria, as did the blocking of MRC1 by MRC1 ligands. The uptake of bacteria by MRC1 delayed the maturation of phagolysosomes. These findings suggest that MRC1 plays an important role in the uptake of various bacteria by TG cells and may provide an opportunity for those bacteria to escape from phagosomes.

Original languageEnglish
Pages (from-to)427-435
Number of pages9
JournalFEMS Immunology and Medical Microbiology
Volume66
Issue number3
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Trophoblasts
Giant Cells
Bacteria
Phagosomes
Immune Tolerance
Listeria monocytogenes
Bacillus subtilis
Placenta
mannose receptor
Stem Cells
Macrophages
Mothers
Escherichia coli
Ligands
Cell Line
Pregnancy

Keywords

  • Abortion
  • Bacterial uptake
  • MRC1
  • Placenta
  • Trophoblast

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Mannose receptor, C type 1 contributes to bacterial uptake by placental trophoblast giant cells. / Hashino, Masanori; Tachibana, Masato; Shimizu, Takashi; Watarai, Masahisa.

In: FEMS Immunology and Medical Microbiology, Vol. 66, No. 3, 2012, p. 427-435.

Research output: Contribution to journalArticle

@article{5c2bd5ac87eb43e4aec7a3d6e9ad38d8,
title = "Mannose receptor, C type 1 contributes to bacterial uptake by placental trophoblast giant cells",
abstract = "During pregnancy, maternal immune function is strictly controlled and immune tolerance is induced. Trophoblast giant (TG) cells exhibit phagocytic activity and show macrophage-like activity against microorganisms in the placenta. However, details of molecular receptors and mechanisms for uptake by TG cells have not been clarified. In this study, we investigated the involvement of the mannose receptor, C type 1 (MRC1), in the uptake of the abortion-inducible bacterium Listeria monocytogenes and abortion-uninducible bacteria Bacillus subtilis and Escherichia coli by TG cells differentiated from a mouse trophoblast stem cell line in vitro. Knockdown of MRC1 inhibited the uptake of all of these bacteria, as did the blocking of MRC1 by MRC1 ligands. The uptake of bacteria by MRC1 delayed the maturation of phagolysosomes. These findings suggest that MRC1 plays an important role in the uptake of various bacteria by TG cells and may provide an opportunity for those bacteria to escape from phagosomes.",
keywords = "Abortion, Bacterial uptake, MRC1, Placenta, Trophoblast",
author = "Masanori Hashino and Masato Tachibana and Takashi Shimizu and Masahisa Watarai",
year = "2012",
doi = "10.1111/1574-695X.12009",
language = "English",
volume = "66",
pages = "427--435",
journal = "Pathogens and Disease",
issn = "2049-632X",
publisher = "John Wiley & Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Mannose receptor, C type 1 contributes to bacterial uptake by placental trophoblast giant cells

AU - Hashino, Masanori

AU - Tachibana, Masato

AU - Shimizu, Takashi

AU - Watarai, Masahisa

PY - 2012

Y1 - 2012

N2 - During pregnancy, maternal immune function is strictly controlled and immune tolerance is induced. Trophoblast giant (TG) cells exhibit phagocytic activity and show macrophage-like activity against microorganisms in the placenta. However, details of molecular receptors and mechanisms for uptake by TG cells have not been clarified. In this study, we investigated the involvement of the mannose receptor, C type 1 (MRC1), in the uptake of the abortion-inducible bacterium Listeria monocytogenes and abortion-uninducible bacteria Bacillus subtilis and Escherichia coli by TG cells differentiated from a mouse trophoblast stem cell line in vitro. Knockdown of MRC1 inhibited the uptake of all of these bacteria, as did the blocking of MRC1 by MRC1 ligands. The uptake of bacteria by MRC1 delayed the maturation of phagolysosomes. These findings suggest that MRC1 plays an important role in the uptake of various bacteria by TG cells and may provide an opportunity for those bacteria to escape from phagosomes.

AB - During pregnancy, maternal immune function is strictly controlled and immune tolerance is induced. Trophoblast giant (TG) cells exhibit phagocytic activity and show macrophage-like activity against microorganisms in the placenta. However, details of molecular receptors and mechanisms for uptake by TG cells have not been clarified. In this study, we investigated the involvement of the mannose receptor, C type 1 (MRC1), in the uptake of the abortion-inducible bacterium Listeria monocytogenes and abortion-uninducible bacteria Bacillus subtilis and Escherichia coli by TG cells differentiated from a mouse trophoblast stem cell line in vitro. Knockdown of MRC1 inhibited the uptake of all of these bacteria, as did the blocking of MRC1 by MRC1 ligands. The uptake of bacteria by MRC1 delayed the maturation of phagolysosomes. These findings suggest that MRC1 plays an important role in the uptake of various bacteria by TG cells and may provide an opportunity for those bacteria to escape from phagosomes.

KW - Abortion

KW - Bacterial uptake

KW - MRC1

KW - Placenta

KW - Trophoblast

UR - http://www.scopus.com/inward/record.url?scp=85027921553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027921553&partnerID=8YFLogxK

U2 - 10.1111/1574-695X.12009

DO - 10.1111/1574-695X.12009

M3 - Article

C2 - 23163874

AN - SCOPUS:85027921553

VL - 66

SP - 427

EP - 435

JO - Pathogens and Disease

JF - Pathogens and Disease

SN - 2049-632X

IS - 3

ER -