Mannitol enhances therapeutic effects of intra-arterial transplantation of mesenchymal stem cells into the brain after traumatic brain injury

Yu Okuma, Feifei Wang, Atsuhiko Toyoshima, Masahiro Kameda, Tomohito Hishikawa, Koji Tokunaga, Kenji Sugiu, Keyue Liu, Jun Haruma, Masahiro Nishibori, Takao Yasuhara, Isao Date

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Traumatic brain injury (TBI) sustained in a traffic accident or a fall is a major cause of death that affects a broad range of ages. The aim of this study was to investigate the therapeutic effects of intra-arterial transplantation of mesenchymal stem cells (MSCs) combined with hypertonic glycerol (25%) or mannitol (25%) in a TBI model of rats. TBI models were produced with a fluid percussion device. At 24h after TBI, MSCs (1×106cells/100μl) with glycerol or mannitol were administered via the right internal carotid artery. Rats were evaluated behaviorally and immunohistochemically, and hyperpermeability of the blood-brain barrier (BBB) induced by hypertonic solutions was explored. Compared to PBS or glycerol, the administration of mannitol resulted in increased BBB disruption. The mannitol-treated rats showed significant improvement in motor function. Intra-arterial transplantation of MSCs caused no thromboembolic ischemia. Immunohistochemically, more MSCs were observed in the injured brain tissues of mannitol-treated rats than in glycerol or PBS-treated rats at 24h after transplantation. Intra-arterial transplantation of MSCs combined with mannitol is an effective treatment in a TBI model of rats. This technique might be used for patients with diseases of the central nervous system including TBI.

Original languageEnglish
Pages (from-to)156-161
Number of pages6
JournalNeuroscience Letters
Volume554
DOIs
Publication statusPublished - Oct 25 2013

Keywords

  • Hypertonic solution
  • Mesenchymal stem cells
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)

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