TY - JOUR
T1 - Mannitol enhances therapeutic effects of intra-arterial transplantation of mesenchymal stem cells into the brain after traumatic brain injury
AU - Okuma, Yu
AU - Wang, Feifei
AU - Toyoshima, Atsuhiko
AU - Kameda, Masahiro
AU - Hishikawa, Tomohito
AU - Tokunaga, Koji
AU - Sugiu, Kenji
AU - Liu, Keyue
AU - Haruma, Jun
AU - Nishibori, Masahiro
AU - Yasuhara, Takao
AU - Date, Isao
N1 - Funding Information:
The authors thank Ms. Masako Arao and Ms. Natsuki Uemori for their assistance. This work was supported in part by Grants-in-Aid for Scientific Research and Health Science Research Grants for Research on Brain Science from the Ministry of Education, Culture, Sports, Science and Technology, Japan .
PY - 2013/10/25
Y1 - 2013/10/25
N2 - Traumatic brain injury (TBI) sustained in a traffic accident or a fall is a major cause of death that affects a broad range of ages. The aim of this study was to investigate the therapeutic effects of intra-arterial transplantation of mesenchymal stem cells (MSCs) combined with hypertonic glycerol (25%) or mannitol (25%) in a TBI model of rats. TBI models were produced with a fluid percussion device. At 24h after TBI, MSCs (1×106cells/100μl) with glycerol or mannitol were administered via the right internal carotid artery. Rats were evaluated behaviorally and immunohistochemically, and hyperpermeability of the blood-brain barrier (BBB) induced by hypertonic solutions was explored. Compared to PBS or glycerol, the administration of mannitol resulted in increased BBB disruption. The mannitol-treated rats showed significant improvement in motor function. Intra-arterial transplantation of MSCs caused no thromboembolic ischemia. Immunohistochemically, more MSCs were observed in the injured brain tissues of mannitol-treated rats than in glycerol or PBS-treated rats at 24h after transplantation. Intra-arterial transplantation of MSCs combined with mannitol is an effective treatment in a TBI model of rats. This technique might be used for patients with diseases of the central nervous system including TBI.
AB - Traumatic brain injury (TBI) sustained in a traffic accident or a fall is a major cause of death that affects a broad range of ages. The aim of this study was to investigate the therapeutic effects of intra-arterial transplantation of mesenchymal stem cells (MSCs) combined with hypertonic glycerol (25%) or mannitol (25%) in a TBI model of rats. TBI models were produced with a fluid percussion device. At 24h after TBI, MSCs (1×106cells/100μl) with glycerol or mannitol were administered via the right internal carotid artery. Rats were evaluated behaviorally and immunohistochemically, and hyperpermeability of the blood-brain barrier (BBB) induced by hypertonic solutions was explored. Compared to PBS or glycerol, the administration of mannitol resulted in increased BBB disruption. The mannitol-treated rats showed significant improvement in motor function. Intra-arterial transplantation of MSCs caused no thromboembolic ischemia. Immunohistochemically, more MSCs were observed in the injured brain tissues of mannitol-treated rats than in glycerol or PBS-treated rats at 24h after transplantation. Intra-arterial transplantation of MSCs combined with mannitol is an effective treatment in a TBI model of rats. This technique might be used for patients with diseases of the central nervous system including TBI.
KW - Hypertonic solution
KW - Mesenchymal stem cells
KW - Traumatic brain injury
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U2 - 10.1016/j.neulet.2013.08.058
DO - 10.1016/j.neulet.2013.08.058
M3 - Article
C2 - 24016413
AN - SCOPUS:84887365441
VL - 554
SP - 156
EP - 161
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
ER -
