Abstract
Biosurfactant-producing bacteria were isolated from mangrove sediment samples collected in the southern part of Thailand by an enrichment-culture technique in which lubricating oil was the sole carbon source. A total of 1,600 colonies were obtained, which were screened for biosurfactant production using the qualitative dropcollapsing test in a mineral salts medium containing 1% of different carbon sources (commercial sugar, glucose, molasses, and used lubricating oil). Ninety-five isolates were positive for biosurfactant production based on the results of this test, among which 20 could reduce the surface tension of the 48-h culture supernatant. The phylogenetic position of these 20 isolates was evaluated by 16S rRNA gene sequence analysis. The production of biosurfactants was determined for strains representative of eight different bacterial genera. Leucobacter komagatae 183, one of the newly isolated strains showing biosurfactant production, produced extracellular biosurfactants which reduced the surface tension of the culture supernatant from 72.0 to 32.0 m/Nm. Eighteen strains released extracellular emulsifiers able to stabilize the emulsion formed. Among these, the strains L. komagatae 183 and Ochrobactrum anthropi 11/6 exhibited emulsification activities comparable to those of synthetic surfactants. Overall, the new biosurfactantproducing strains isolated in this study display promising features for the future development and use in economically efficient industrial-scale biotechnological processes.
| Original language | English |
|---|---|
| Pages (from-to) | 1669-1679 |
| Number of pages | 11 |
| Journal | Annals of Microbiology |
| Volume | 62 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 2012 |
Fingerprint
Keywords
- Bioemulsifier
- Biosurfactant
- Mangrove sediment
- Renewable substrate
- Screening
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
Cite this
Mangrove sediment, a new source of potential biosurfactant-producing bacteria. / Saimmai, Atipan; Tani, Akio; Sobhon, Vorasan; Maneerat, Suppasil.
In: Annals of Microbiology, Vol. 62, No. 4, 12.2012, p. 1669-1679.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mangrove sediment, a new source of potential biosurfactant-producing bacteria
AU - Saimmai, Atipan
AU - Tani, Akio
AU - Sobhon, Vorasan
AU - Maneerat, Suppasil
PY - 2012/12
Y1 - 2012/12
N2 - Biosurfactant-producing bacteria were isolated from mangrove sediment samples collected in the southern part of Thailand by an enrichment-culture technique in which lubricating oil was the sole carbon source. A total of 1,600 colonies were obtained, which were screened for biosurfactant production using the qualitative dropcollapsing test in a mineral salts medium containing 1% of different carbon sources (commercial sugar, glucose, molasses, and used lubricating oil). Ninety-five isolates were positive for biosurfactant production based on the results of this test, among which 20 could reduce the surface tension of the 48-h culture supernatant. The phylogenetic position of these 20 isolates was evaluated by 16S rRNA gene sequence analysis. The production of biosurfactants was determined for strains representative of eight different bacterial genera. Leucobacter komagatae 183, one of the newly isolated strains showing biosurfactant production, produced extracellular biosurfactants which reduced the surface tension of the culture supernatant from 72.0 to 32.0 m/Nm. Eighteen strains released extracellular emulsifiers able to stabilize the emulsion formed. Among these, the strains L. komagatae 183 and Ochrobactrum anthropi 11/6 exhibited emulsification activities comparable to those of synthetic surfactants. Overall, the new biosurfactantproducing strains isolated in this study display promising features for the future development and use in economically efficient industrial-scale biotechnological processes.
AB - Biosurfactant-producing bacteria were isolated from mangrove sediment samples collected in the southern part of Thailand by an enrichment-culture technique in which lubricating oil was the sole carbon source. A total of 1,600 colonies were obtained, which were screened for biosurfactant production using the qualitative dropcollapsing test in a mineral salts medium containing 1% of different carbon sources (commercial sugar, glucose, molasses, and used lubricating oil). Ninety-five isolates were positive for biosurfactant production based on the results of this test, among which 20 could reduce the surface tension of the 48-h culture supernatant. The phylogenetic position of these 20 isolates was evaluated by 16S rRNA gene sequence analysis. The production of biosurfactants was determined for strains representative of eight different bacterial genera. Leucobacter komagatae 183, one of the newly isolated strains showing biosurfactant production, produced extracellular biosurfactants which reduced the surface tension of the culture supernatant from 72.0 to 32.0 m/Nm. Eighteen strains released extracellular emulsifiers able to stabilize the emulsion formed. Among these, the strains L. komagatae 183 and Ochrobactrum anthropi 11/6 exhibited emulsification activities comparable to those of synthetic surfactants. Overall, the new biosurfactantproducing strains isolated in this study display promising features for the future development and use in economically efficient industrial-scale biotechnological processes.
KW - Bioemulsifier
KW - Biosurfactant
KW - Mangrove sediment
KW - Renewable substrate
KW - Screening
UR - http://www.scopus.com/inward/record.url?scp=84871457126&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871457126&partnerID=8YFLogxK
U2 - 10.1007/s13213-012-0424-9
DO - 10.1007/s13213-012-0424-9
M3 - Article
AN - SCOPUS:84871457126
VL - 62
SP - 1669
EP - 1679
JO - Annals of Microbiology
JF - Annals of Microbiology
SN - 1590-4261
IS - 4
ER -