Mangrove sediment, a new source of potential biosurfactant-producing bacteria

Atipan Saimmai, Akio Tani, Vorasan Sobhon, Suppasil Maneerat

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Biosurfactant-producing bacteria were isolated from mangrove sediment samples collected in the southern part of Thailand by an enrichment-culture technique in which lubricating oil was the sole carbon source. A total of 1,600 colonies were obtained, which were screened for biosurfactant production using the qualitative dropcollapsing test in a mineral salts medium containing 1% of different carbon sources (commercial sugar, glucose, molasses, and used lubricating oil). Ninety-five isolates were positive for biosurfactant production based on the results of this test, among which 20 could reduce the surface tension of the 48-h culture supernatant. The phylogenetic position of these 20 isolates was evaluated by 16S rRNA gene sequence analysis. The production of biosurfactants was determined for strains representative of eight different bacterial genera. Leucobacter komagatae 183, one of the newly isolated strains showing biosurfactant production, produced extracellular biosurfactants which reduced the surface tension of the culture supernatant from 72.0 to 32.0 m/Nm. Eighteen strains released extracellular emulsifiers able to stabilize the emulsion formed. Among these, the strains L. komagatae 183 and Ochrobactrum anthropi 11/6 exhibited emulsification activities comparable to those of synthetic surfactants. Overall, the new biosurfactantproducing strains isolated in this study display promising features for the future development and use in economically efficient industrial-scale biotechnological processes.

Original languageEnglish
Pages (from-to)1669-1679
Number of pages11
JournalAnnals of Microbiology
Volume62
Issue number4
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Surface Tension
Ochrobactrum anthropi
Oils
Carbon
Bacteria
Molasses
Culture Techniques
Thailand
Emulsions
rRNA Genes
Surface-Active Agents
Minerals
Sequence Analysis
Salts
Glucose

Keywords

  • Bioemulsifier
  • Biosurfactant
  • Mangrove sediment
  • Renewable substrate
  • Screening

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Mangrove sediment, a new source of potential biosurfactant-producing bacteria. / Saimmai, Atipan; Tani, Akio; Sobhon, Vorasan; Maneerat, Suppasil.

In: Annals of Microbiology, Vol. 62, No. 4, 12.2012, p. 1669-1679.

Research output: Contribution to journalArticle

Saimmai, Atipan ; Tani, Akio ; Sobhon, Vorasan ; Maneerat, Suppasil. / Mangrove sediment, a new source of potential biosurfactant-producing bacteria. In: Annals of Microbiology. 2012 ; Vol. 62, No. 4. pp. 1669-1679.
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