Abstract
Malignant lymphoma was induced in Japanese (JWY), New Zealand (NZY) and Dutch (DUY) white rabbits by oral spray of cell-free pellets of culture fluid (crude virus fraction) of Ts-B6 cells (cynomolgus monkey B-lymphoblastoid cells harboring Epstein Barr virus-related simian herpesvirus or Cyno-EBV). Nine of 11 inoculated rabbits developed malignant lymphomas within 42-160 days after oral inoculation (JWY, 2/3; NZY, 5/6; DUY, 2/2). In contrast, none of the control rabbits inoculated in the same fashion with B95-8 (EBV-producing marmoset cell line) cell-free pellets developed malignant lymphoma. Most rabbits showed increased anti-VCA IgG and anti-EA-DR IgG antibody titers after inoculation by oral spray of Ts-B6 cell-free pellets. EBV-encoded RNA-1 was revealed in the tumor cells by in situ hybridization. EBV DNA was detected in the rabbit peripheral blood leukocytes (PBL) by polymerase chain reaction; the earliest positive result was obtained only two days after oral inoculation. These data suggest that orally administered Cyno-EBV in Ts-B6 cells infects PBL and then induces malignant lymphoma in rabbits. The availability of this animal model promises to clarify the role of EBV in human lymphoma and provides a means for studying prophylactic and therapeutic regimens.
| Original language | English |
|---|---|
| Pages (from-to) | 141-147 |
| Number of pages | 7 |
| Journal | Acta Medica Okayama |
| Volume | 51 |
| Issue number | 3 |
| Publication status | Published - Jun 1997 |
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Keywords
- Animal model
- Epstein-Barr virus
- Malignant lymphoma
- Oral inoculation
- Simian herpesvirus
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Malignant Lymphoma Induction in Rabbits by Oral Inoculation of Crude Virus Fraction Prepared from Ts-B6 Cells (Cynomolgus B-Lymphoblastoid Cells Harboring Epstein-Barr Virus-Related Simian Herpesvirus). / Chen, Hong Li; Hayashi, Kazuhiko; Koirala, Tirtha Raj; Ino, Hideo; Fujimoto, Koji; Yoshikawa, Yasuhiro; Choudhury, Chitta Ranjan; Akagi, Tadaatsu.
In: Acta Medica Okayama, Vol. 51, No. 3, 06.1997, p. 141-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Malignant Lymphoma Induction in Rabbits by Oral Inoculation of Crude Virus Fraction Prepared from Ts-B6 Cells (Cynomolgus B-Lymphoblastoid Cells Harboring Epstein-Barr Virus-Related Simian Herpesvirus)
AU - Chen, Hong Li
AU - Hayashi, Kazuhiko
AU - Koirala, Tirtha Raj
AU - Ino, Hideo
AU - Fujimoto, Koji
AU - Yoshikawa, Yasuhiro
AU - Choudhury, Chitta Ranjan
AU - Akagi, Tadaatsu
PY - 1997/6
Y1 - 1997/6
N2 - Malignant lymphoma was induced in Japanese (JWY), New Zealand (NZY) and Dutch (DUY) white rabbits by oral spray of cell-free pellets of culture fluid (crude virus fraction) of Ts-B6 cells (cynomolgus monkey B-lymphoblastoid cells harboring Epstein Barr virus-related simian herpesvirus or Cyno-EBV). Nine of 11 inoculated rabbits developed malignant lymphomas within 42-160 days after oral inoculation (JWY, 2/3; NZY, 5/6; DUY, 2/2). In contrast, none of the control rabbits inoculated in the same fashion with B95-8 (EBV-producing marmoset cell line) cell-free pellets developed malignant lymphoma. Most rabbits showed increased anti-VCA IgG and anti-EA-DR IgG antibody titers after inoculation by oral spray of Ts-B6 cell-free pellets. EBV-encoded RNA-1 was revealed in the tumor cells by in situ hybridization. EBV DNA was detected in the rabbit peripheral blood leukocytes (PBL) by polymerase chain reaction; the earliest positive result was obtained only two days after oral inoculation. These data suggest that orally administered Cyno-EBV in Ts-B6 cells infects PBL and then induces malignant lymphoma in rabbits. The availability of this animal model promises to clarify the role of EBV in human lymphoma and provides a means for studying prophylactic and therapeutic regimens.
AB - Malignant lymphoma was induced in Japanese (JWY), New Zealand (NZY) and Dutch (DUY) white rabbits by oral spray of cell-free pellets of culture fluid (crude virus fraction) of Ts-B6 cells (cynomolgus monkey B-lymphoblastoid cells harboring Epstein Barr virus-related simian herpesvirus or Cyno-EBV). Nine of 11 inoculated rabbits developed malignant lymphomas within 42-160 days after oral inoculation (JWY, 2/3; NZY, 5/6; DUY, 2/2). In contrast, none of the control rabbits inoculated in the same fashion with B95-8 (EBV-producing marmoset cell line) cell-free pellets developed malignant lymphoma. Most rabbits showed increased anti-VCA IgG and anti-EA-DR IgG antibody titers after inoculation by oral spray of Ts-B6 cell-free pellets. EBV-encoded RNA-1 was revealed in the tumor cells by in situ hybridization. EBV DNA was detected in the rabbit peripheral blood leukocytes (PBL) by polymerase chain reaction; the earliest positive result was obtained only two days after oral inoculation. These data suggest that orally administered Cyno-EBV in Ts-B6 cells infects PBL and then induces malignant lymphoma in rabbits. The availability of this animal model promises to clarify the role of EBV in human lymphoma and provides a means for studying prophylactic and therapeutic regimens.
KW - Animal model
KW - Epstein-Barr virus
KW - Malignant lymphoma
KW - Oral inoculation
KW - Simian herpesvirus
UR - http://www.scopus.com/inward/record.url?scp=0031155160&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031155160&partnerID=8YFLogxK
M3 - Article
C2 - 9227793
AN - SCOPUS:0031155160
VL - 51
SP - 141
EP - 147
JO - Acta Medica Okayama
JF - Acta Medica Okayama
SN - 0386-300X
IS - 3
ER -