Malignant Lymphoma Induction in Rabbits by Oral Inoculation of Crude Virus Fraction Prepared from Ts-B6 Cells (Cynomolgus B-Lymphoblastoid Cells Harboring Epstein-Barr Virus-Related Simian Herpesvirus)

Hong Li Chen, Kazuhiko Hayashi, Tirtha Raj Koirala, Hideo Ino, Koji Fujimoto, Yasuhiro Yoshikawa, Chitta Ranjan Choudhury, Tadaatsu Akagi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Malignant lymphoma was induced in Japanese (JWY), New Zealand (NZY) and Dutch (DUY) white rabbits by oral spray of cell-free pellets of culture fluid (crude virus fraction) of Ts-B6 cells (cynomolgus monkey B-lymphoblastoid cells harboring Epstein Barr virus-related simian herpesvirus or Cyno-EBV). Nine of 11 inoculated rabbits developed malignant lymphomas within 42-160 days after oral inoculation (JWY, 2/3; NZY, 5/6; DUY, 2/2). In contrast, none of the control rabbits inoculated in the same fashion with B95-8 (EBV-producing marmoset cell line) cell-free pellets developed malignant lymphoma. Most rabbits showed increased anti-VCA IgG and anti-EA-DR IgG antibody titers after inoculation by oral spray of Ts-B6 cell-free pellets. EBV-encoded RNA-1 was revealed in the tumor cells by in situ hybridization. EBV DNA was detected in the rabbit peripheral blood leukocytes (PBL) by polymerase chain reaction; the earliest positive result was obtained only two days after oral inoculation. These data suggest that orally administered Cyno-EBV in Ts-B6 cells infects PBL and then induces malignant lymphoma in rabbits. The availability of this animal model promises to clarify the role of EBV in human lymphoma and provides a means for studying prophylactic and therapeutic regimens.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalActa medica Okayama
Volume51
Issue number3
Publication statusPublished - Jun 1 1997

Keywords

  • Animal model
  • Epstein-Barr virus
  • Malignant lymphoma
  • Oral inoculation
  • Simian herpesvirus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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