Abstract
Magnetic immunoassays utilizing magnetic marker and high Tc superconducting quantum interference device (SQUID) have been performed. In this magnetic method, binding-reaction between an antigen and its antibody is detected by measuring the magnetic field from the magnetic marker. First, we discuss the magnetic property of the marker, and show that Fe3O 4 particles with diameter of 25 nm can be used for remanence measurement. We also show a design of the SQUID for sensitive detection of the magnetic signal from the marker. Next, we developed a measurement system utilizing the SQUID and a reaction chamber with very low magnetic contamination. Finally, we conducted an experiment on the detection of the biological materials called IL8 and IgE. At present, a few atto-mol of IL8 and IgE has been detected, which shows the high sensitivity of the present method.
| Original language | English |
|---|---|
| Pages (from-to) | 158-165 |
| Number of pages | 8 |
| Journal | IEICE Transactions on Electronics |
| Volume | E88-C |
| Issue number | 2 |
| Publication status | Published - 2005 |
| Externally published | Yes |
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Keywords
- High T SQUID
- Immunoassay
- Magnetic marker
- Magnetic nanoparticle
- Remanence
ASJC Scopus subject areas
- Electrical and Electronic Engineering
Cite this
Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays. / Enpuku, Keiji; Inoue, Katsuhiro; Yoshinaga, Kohji; Tsukamoto, Akira; Saitoh, Kazuo; Tsukada, Keiji; Kandori, Akihiko; Sugiura, Yoshinori; Hamaoka, Shigenori; Morita, Hiroyuki; Kuma, Hiroyuki; Hamasaki, Naotaka.
In: IEICE Transactions on Electronics, Vol. E88-C, No. 2, 2005, p. 158-165.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays
AU - Enpuku, Keiji
AU - Inoue, Katsuhiro
AU - Yoshinaga, Kohji
AU - Tsukamoto, Akira
AU - Saitoh, Kazuo
AU - Tsukada, Keiji
AU - Kandori, Akihiko
AU - Sugiura, Yoshinori
AU - Hamaoka, Shigenori
AU - Morita, Hiroyuki
AU - Kuma, Hiroyuki
AU - Hamasaki, Naotaka
PY - 2005
Y1 - 2005
N2 - Magnetic immunoassays utilizing magnetic marker and high Tc superconducting quantum interference device (SQUID) have been performed. In this magnetic method, binding-reaction between an antigen and its antibody is detected by measuring the magnetic field from the magnetic marker. First, we discuss the magnetic property of the marker, and show that Fe3O 4 particles with diameter of 25 nm can be used for remanence measurement. We also show a design of the SQUID for sensitive detection of the magnetic signal from the marker. Next, we developed a measurement system utilizing the SQUID and a reaction chamber with very low magnetic contamination. Finally, we conducted an experiment on the detection of the biological materials called IL8 and IgE. At present, a few atto-mol of IL8 and IgE has been detected, which shows the high sensitivity of the present method.
AB - Magnetic immunoassays utilizing magnetic marker and high Tc superconducting quantum interference device (SQUID) have been performed. In this magnetic method, binding-reaction between an antigen and its antibody is detected by measuring the magnetic field from the magnetic marker. First, we discuss the magnetic property of the marker, and show that Fe3O 4 particles with diameter of 25 nm can be used for remanence measurement. We also show a design of the SQUID for sensitive detection of the magnetic signal from the marker. Next, we developed a measurement system utilizing the SQUID and a reaction chamber with very low magnetic contamination. Finally, we conducted an experiment on the detection of the biological materials called IL8 and IgE. At present, a few atto-mol of IL8 and IgE has been detected, which shows the high sensitivity of the present method.
KW - High T SQUID
KW - Immunoassay
KW - Magnetic marker
KW - Magnetic nanoparticle
KW - Remanence
UR - http://www.scopus.com/inward/record.url?scp=24144431637&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24144431637&partnerID=8YFLogxK
M3 - Article
VL - E88-C
SP - 158
EP - 165
JO - IEICE Transactions on Electronics
JF - IEICE Transactions on Electronics
SN - 0916-8524
IS - 2
ER -