Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays

Keiji Enpuku; Katsuhiro Inoue; Kohji Yoshinaga; Akira Tsukamoto; Kazuo Saitoh; Keiji Tsukada; Akihiko Kandori; Yoshinori Sugiura; Shigenori Hamaoka; Hiroyuki Morita; Hiroyuki Kuma; Naotaka Hamasaki

Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays

Keiji Enpuku, Katsuhiro Inoue, Kohji Yoshinaga, Akira Tsukamoto, Kazuo Saitoh, Keiji Tsukada, Akihiko Kandori, Yoshinori Sugiura, Shigenori Hamaoka, Hiroyuki Morita, Hiroyuki Kuma, Naotaka Hamasaki

Research output: Contribution to journal › Article

15 Citations (Scopus)

Abstract

Magnetic immunoassays utilizing magnetic marker and high T_c superconducting quantum interference device (SQUID) have been performed. In this magnetic method, binding-reaction between an antigen and its antibody is detected by measuring the magnetic field from the magnetic marker. First, we discuss the magnetic property of the marker, and show that Fe₃O ₄ particles with diameter of 25 nm can be used for remanence measurement. We also show a design of the SQUID for sensitive detection of the magnetic signal from the marker. Next, we developed a measurement system utilizing the SQUID and a reaction chamber with very low magnetic contamination. Finally, we conducted an experiment on the detection of the biological materials called IL8 and IgE. At present, a few atto-mol of IL8 and IgE has been detected, which shows the high sensitivity of the present method.

Original language	English
Pages (from-to)	158-165
Number of pages	8
Journal	IEICE Transactions on Electronics
Volume	E88-C
Issue number	2
Publication status	Published - 2005
Externally published	Yes

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Keywords

High T SQUID
Immunoassay
Magnetic marker
Magnetic nanoparticle
Remanence

ASJC Scopus subject areas

Electrical and Electronic Engineering

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Enpuku, K., Inoue, K., Yoshinaga, K., Tsukamoto, A., Saitoh, K., Tsukada, K., Kandori, A., Sugiura, Y., Hamaoka, S., Morita, H., Kuma, H., & Hamasaki, N. (2005). Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays. IEICE Transactions on Electronics, E88-C(2), 158-165.

Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays. / Enpuku, Keiji; Inoue, Katsuhiro; Yoshinaga, Kohji; Tsukamoto, Akira; Saitoh, Kazuo; Tsukada, Keiji; Kandori, Akihiko; Sugiura, Yoshinori; Hamaoka, Shigenori; Morita, Hiroyuki; Kuma, Hiroyuki; Hamasaki, Naotaka.

In: IEICE Transactions on Electronics, Vol. E88-C, No. 2, 2005, p. 158-165.

Research output: Contribution to journal › Article

Enpuku, Keiji ; Inoue, Katsuhiro ; Yoshinaga, Kohji ; Tsukamoto, Akira ; Saitoh, Kazuo ; Tsukada, Keiji ; Kandori, Akihiko ; Sugiura, Yoshinori ; Hamaoka, Shigenori ; Morita, Hiroyuki ; Kuma, Hiroyuki ; Hamasaki, Naotaka. / Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays. In: IEICE Transactions on Electronics. 2005 ; Vol. E88-C, No. 2. pp. 158-165.

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abstract = "Magnetic immunoassays utilizing magnetic marker and high Tc superconducting quantum interference device (SQUID) have been performed. In this magnetic method, binding-reaction between an antigen and its antibody is detected by measuring the magnetic field from the magnetic marker. First, we discuss the magnetic property of the marker, and show that Fe3O 4 particles with diameter of 25 nm can be used for remanence measurement. We also show a design of the SQUID for sensitive detection of the magnetic signal from the marker. Next, we developed a measurement system utilizing the SQUID and a reaction chamber with very low magnetic contamination. Finally, we conducted an experiment on the detection of the biological materials called IL8 and IgE. At present, a few atto-mol of IL8 and IgE has been detected, which shows the high sensitivity of the present method.",

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AU - Enpuku, Keiji

AU - Inoue, Katsuhiro

AU - Yoshinaga, Kohji

AU - Tsukamoto, Akira

AU - Saitoh, Kazuo

AU - Tsukada, Keiji

AU - Kandori, Akihiko

AU - Sugiura, Yoshinori

AU - Hamaoka, Shigenori

AU - Morita, Hiroyuki

AU - Kuma, Hiroyuki

AU - Hamasaki, Naotaka

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AB - Magnetic immunoassays utilizing magnetic marker and high Tc superconducting quantum interference device (SQUID) have been performed. In this magnetic method, binding-reaction between an antigen and its antibody is detected by measuring the magnetic field from the magnetic marker. First, we discuss the magnetic property of the marker, and show that Fe3O 4 particles with diameter of 25 nm can be used for remanence measurement. We also show a design of the SQUID for sensitive detection of the magnetic signal from the marker. Next, we developed a measurement system utilizing the SQUID and a reaction chamber with very low magnetic contamination. Finally, we conducted an experiment on the detection of the biological materials called IL8 and IgE. At present, a few atto-mol of IL8 and IgE has been detected, which shows the high sensitivity of the present method.

KW - High T SQUID

KW - Immunoassay

KW - Magnetic marker

KW - Magnetic nanoparticle

KW - Remanence

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SN - 0916-8524

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ER -

Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays

Abstract

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Keywords

ASJC Scopus subject areas

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