Macroscopic characterization of bilayer membranes composed of triglyceride and phosphatidylcholine investigated using high-pressure ESR spin probe technique

Yoshimi Sueishi, Shinpei Iwamoto, Keitaro Miyazono, Shinichiro Nakatani, Kouichi Nakagawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effect of high static pressure on the microscopic properties of triglyceride (HCO-10) and phosphatidylcholine (DLPC) membranes was investigated by ESR (electron spin resonance). The external pressure was varied to ascertain the distribution equilibrium constant between the aqueous and vesicle phases, depending on the molecular sizes of various nitroxide probes relative to that of the interstitial space of the HCO-10 and DLPC membranes. The distribution equilibrium of any probe for the HCO-10 membrane was shifted to the aqueous phase side with increasing the external pressure. However, the distribution equilibrium of di-tert-butyl nitroxide (DTBN) for the DLPC membrane was shifted to the vesicle phase side with increasing external pressure. Furthermore, information on the rotational motion of the nitroxide probes in the HCO-10 and DLPC vesicle was obtained from the anisotropic ESR signals. Rotational correlation times (τR) of the nitroxide probes in the vesicle phases increased with increasing external pressure. The magnitude of the activation volume (ΔV) obtained using τR in both vesicles is TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl)>DTBN>DMDEN (2,2-dimethyl-5,5-diethylpyrroline N-oxide), which might be attributed to the interaction between the probes and the membrane chains.

Original languageEnglish
Pages (from-to)262-267
Number of pages6
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume415
DOIs
Publication statusPublished - 2012

Fingerprint

Phosphatidylcholines
Paramagnetic resonance
electron paramagnetic resonance
Triglycerides
membranes
Membranes
probes
static pressure
Equilibrium constants
Oxides
interstitials
Chemical activation
activation
oxides
interactions

Keywords

  • Bilayer membrane
  • ESR
  • High pressure
  • Phosphatidylcholine
  • Spin probe
  • Triglyceride

ASJC Scopus subject areas

  • Colloid and Surface Chemistry

Cite this

Macroscopic characterization of bilayer membranes composed of triglyceride and phosphatidylcholine investigated using high-pressure ESR spin probe technique. / Sueishi, Yoshimi; Iwamoto, Shinpei; Miyazono, Keitaro; Nakatani, Shinichiro; Nakagawa, Kouichi.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 415, 2012, p. 262-267.

Research output: Contribution to journalArticle

@article{bd3c279f6a854e0d85f4b2630a066894,
title = "Macroscopic characterization of bilayer membranes composed of triglyceride and phosphatidylcholine investigated using high-pressure ESR spin probe technique",
abstract = "The effect of high static pressure on the microscopic properties of triglyceride (HCO-10) and phosphatidylcholine (DLPC) membranes was investigated by ESR (electron spin resonance). The external pressure was varied to ascertain the distribution equilibrium constant between the aqueous and vesicle phases, depending on the molecular sizes of various nitroxide probes relative to that of the interstitial space of the HCO-10 and DLPC membranes. The distribution equilibrium of any probe for the HCO-10 membrane was shifted to the aqueous phase side with increasing the external pressure. However, the distribution equilibrium of di-tert-butyl nitroxide (DTBN) for the DLPC membrane was shifted to the vesicle phase side with increasing external pressure. Furthermore, information on the rotational motion of the nitroxide probes in the HCO-10 and DLPC vesicle was obtained from the anisotropic ESR signals. Rotational correlation times (τR) of the nitroxide probes in the vesicle phases increased with increasing external pressure. The magnitude of the activation volume (ΔV‡) obtained using τR in both vesicles is TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl)>DTBN>DMDEN (2,2-dimethyl-5,5-diethylpyrroline N-oxide), which might be attributed to the interaction between the probes and the membrane chains.",
keywords = "Bilayer membrane, ESR, High pressure, Phosphatidylcholine, Spin probe, Triglyceride",
author = "Yoshimi Sueishi and Shinpei Iwamoto and Keitaro Miyazono and Shinichiro Nakatani and Kouichi Nakagawa",
year = "2012",
doi = "10.1016/j.colsurfa.2012.08.065",
language = "English",
volume = "415",
pages = "262--267",
journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",
issn = "0927-7757",
publisher = "Elsevier",

}

TY - JOUR

T1 - Macroscopic characterization of bilayer membranes composed of triglyceride and phosphatidylcholine investigated using high-pressure ESR spin probe technique

AU - Sueishi, Yoshimi

AU - Iwamoto, Shinpei

AU - Miyazono, Keitaro

AU - Nakatani, Shinichiro

AU - Nakagawa, Kouichi

PY - 2012

Y1 - 2012

N2 - The effect of high static pressure on the microscopic properties of triglyceride (HCO-10) and phosphatidylcholine (DLPC) membranes was investigated by ESR (electron spin resonance). The external pressure was varied to ascertain the distribution equilibrium constant between the aqueous and vesicle phases, depending on the molecular sizes of various nitroxide probes relative to that of the interstitial space of the HCO-10 and DLPC membranes. The distribution equilibrium of any probe for the HCO-10 membrane was shifted to the aqueous phase side with increasing the external pressure. However, the distribution equilibrium of di-tert-butyl nitroxide (DTBN) for the DLPC membrane was shifted to the vesicle phase side with increasing external pressure. Furthermore, information on the rotational motion of the nitroxide probes in the HCO-10 and DLPC vesicle was obtained from the anisotropic ESR signals. Rotational correlation times (τR) of the nitroxide probes in the vesicle phases increased with increasing external pressure. The magnitude of the activation volume (ΔV‡) obtained using τR in both vesicles is TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl)>DTBN>DMDEN (2,2-dimethyl-5,5-diethylpyrroline N-oxide), which might be attributed to the interaction between the probes and the membrane chains.

AB - The effect of high static pressure on the microscopic properties of triglyceride (HCO-10) and phosphatidylcholine (DLPC) membranes was investigated by ESR (electron spin resonance). The external pressure was varied to ascertain the distribution equilibrium constant between the aqueous and vesicle phases, depending on the molecular sizes of various nitroxide probes relative to that of the interstitial space of the HCO-10 and DLPC membranes. The distribution equilibrium of any probe for the HCO-10 membrane was shifted to the aqueous phase side with increasing the external pressure. However, the distribution equilibrium of di-tert-butyl nitroxide (DTBN) for the DLPC membrane was shifted to the vesicle phase side with increasing external pressure. Furthermore, information on the rotational motion of the nitroxide probes in the HCO-10 and DLPC vesicle was obtained from the anisotropic ESR signals. Rotational correlation times (τR) of the nitroxide probes in the vesicle phases increased with increasing external pressure. The magnitude of the activation volume (ΔV‡) obtained using τR in both vesicles is TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl)>DTBN>DMDEN (2,2-dimethyl-5,5-diethylpyrroline N-oxide), which might be attributed to the interaction between the probes and the membrane chains.

KW - Bilayer membrane

KW - ESR

KW - High pressure

KW - Phosphatidylcholine

KW - Spin probe

KW - Triglyceride

UR - http://www.scopus.com/inward/record.url?scp=84870271578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870271578&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfa.2012.08.065

DO - 10.1016/j.colsurfa.2012.08.065

M3 - Article

VL - 415

SP - 262

EP - 267

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

ER -