TY - JOUR
T1 - Macrophages cultured in vitro release leukotriene B4 and neutrophil attractant/activation protein (interleukin 8) sequentially in response to stimulation with lipopolysaccharide and zymosan
AU - Rankin, John A.
AU - Sylvester, Ilona
AU - Smith, Sharon
AU - Yoshimura, T.
AU - Leonard, Edward J.
PY - 1990/11
Y1 - 1990/11
N2 - The capacity of lipopolysaccharide (LPS), zymosan, arid calcium ionophore A23187 to induce neutrophil chemotactic activity (NCA), leukotriene B4 (LTB4), and neutrophil attractant/activation protein (NAP-1) release from human alveolar macrophages (AM) retrieved from normal nonsmokers was evaluated. LPS induced a dose-dependent release Of LTB4 that began by 1 h, 4.0±3.2 ng/106 viable AM; peaked at 3 h, 24.7±13.5 ng/106 viable AM; and decreased by 24 h, 1.2±1.0 ng/106 viable AM (n = 8). Quantities of LTB4 in cell-free supernatants of AM stimulated with LPS were determined by reverse-phase high-performance liquid chromatography and corresponded well with results obtained by radioimmunoassay. By contrast, NAP-1 release began ∼ 3-5 h after stimulation of AM with LPS, 197±192 ng/ml, and peaked at 24 h, 790±124 ng/ml. Release of NAP-1 was stimulus specific because A23187 evoked the release of LTB4 but not NAP-1, whereas LPS and zymosan induced the release of both LTB4 and NAP-1. The appearance of neutrophil chemotactic activity in supernatants of AM challenged with LPS for 3 h could be explained completely by the quantities of LTB4 present. After stimulation with LPS or zymosan for 24 h, AM had metabolized almost all generated LTB4. Preincubation of AM with nordihydroguiaretic acid (10-4 M) completely abolished the appearance of NCA, LTB4, and NAP-1 in supernatants of AM challenged with LPS. Therefore, LPS and zymosan particles were potent stimuli of the sequential release of LTB4 and NAP-I from AM.
AB - The capacity of lipopolysaccharide (LPS), zymosan, arid calcium ionophore A23187 to induce neutrophil chemotactic activity (NCA), leukotriene B4 (LTB4), and neutrophil attractant/activation protein (NAP-1) release from human alveolar macrophages (AM) retrieved from normal nonsmokers was evaluated. LPS induced a dose-dependent release Of LTB4 that began by 1 h, 4.0±3.2 ng/106 viable AM; peaked at 3 h, 24.7±13.5 ng/106 viable AM; and decreased by 24 h, 1.2±1.0 ng/106 viable AM (n = 8). Quantities of LTB4 in cell-free supernatants of AM stimulated with LPS were determined by reverse-phase high-performance liquid chromatography and corresponded well with results obtained by radioimmunoassay. By contrast, NAP-1 release began ∼ 3-5 h after stimulation of AM with LPS, 197±192 ng/ml, and peaked at 24 h, 790±124 ng/ml. Release of NAP-1 was stimulus specific because A23187 evoked the release of LTB4 but not NAP-1, whereas LPS and zymosan induced the release of both LTB4 and NAP-1. The appearance of neutrophil chemotactic activity in supernatants of AM challenged with LPS for 3 h could be explained completely by the quantities of LTB4 present. After stimulation with LPS or zymosan for 24 h, AM had metabolized almost all generated LTB4. Preincubation of AM with nordihydroguiaretic acid (10-4 M) completely abolished the appearance of NCA, LTB4, and NAP-1 in supernatants of AM challenged with LPS. Therefore, LPS and zymosan particles were potent stimuli of the sequential release of LTB4 and NAP-I from AM.
KW - Alveolar macrophages
KW - Interleukin 8
KW - Leukotriene B
KW - Lipopolysaccharide
KW - Neutrophil attractant/activation protein
KW - Zymosan
UR - http://www.scopus.com/inward/record.url?scp=0025016233&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025016233&partnerID=8YFLogxK
U2 - 10.1172/JCI114875
DO - 10.1172/JCI114875
M3 - Article
C2 - 2173722
AN - SCOPUS:0025016233
VL - 86
SP - 1556
EP - 1564
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 5
ER -