Macrophages cultured in vitro release leukotriene B4 and neutrophil attractant/activation protein (interleukin 8) sequentially in response to stimulation with lipopolysaccharide and zymosan

John A. Rankin, Ilona Sylvester, Sharon Smith, Teizo Yoshimura, Edward J. Leonard

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The capacity of lipopolysaccharide (LPS), zymosan, arid calcium ionophore A23187 to induce neutrophil chemotactic activity (NCA), leukotriene B4 (LTB4), and neutrophil attractant/activation protein (NAP-1) release from human alveolar macrophages (AM) retrieved from normal nonsmokers was evaluated. LPS induced a dose-dependent release Of LTB4 that began by 1 h, 4.0±3.2 ng/106 viable AM; peaked at 3 h, 24.7±13.5 ng/106 viable AM; and decreased by 24 h, 1.2±1.0 ng/106 viable AM (n = 8). Quantities of LTB4 in cell-free supernatants of AM stimulated with LPS were determined by reverse-phase high-performance liquid chromatography and corresponded well with results obtained by radioimmunoassay. By contrast, NAP-1 release began ∼ 3-5 h after stimulation of AM with LPS, 197±192 ng/ml, and peaked at 24 h, 790±124 ng/ml. Release of NAP-1 was stimulus specific because A23187 evoked the release of LTB4 but not NAP-1, whereas LPS and zymosan induced the release of both LTB4 and NAP-1. The appearance of neutrophil chemotactic activity in supernatants of AM challenged with LPS for 3 h could be explained completely by the quantities of LTB4 present. After stimulation with LPS or zymosan for 24 h, AM had metabolized almost all generated LTB4. Preincubation of AM with nordihydroguiaretic acid (10-4 M) completely abolished the appearance of NCA, LTB4, and NAP-1 in supernatants of AM challenged with LPS. Therefore, LPS and zymosan particles were potent stimuli of the sequential release of LTB4 and NAP-I from AM.

Original languageEnglish
Pages (from-to)1556-1564
Number of pages9
JournalJournal of Clinical Investigation
Volume86
Issue number5
Publication statusPublished - Nov 1990
Externally publishedYes

Fingerprint

Neutrophil Activation
Zymosan
Leukotriene B4
Alveolar Macrophages
Interleukin-8
Lipopolysaccharides
Macrophages
Proteins
Neutrophils
Calcimycin
In Vitro Techniques
Calcium Ionophores
Reverse-Phase Chromatography
Radioimmunoassay
1-nitro-2-acetylpyrrole
High Pressure Liquid Chromatography

Keywords

  • Alveolar macrophages
  • Interleukin 8
  • Leukotriene B
  • Lipopolysaccharide
  • Neutrophil attractant/activation protein
  • Zymosan

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Macrophages cultured in vitro release leukotriene B4 and neutrophil attractant/activation protein (interleukin 8) sequentially in response to stimulation with lipopolysaccharide and zymosan. / Rankin, John A.; Sylvester, Ilona; Smith, Sharon; Yoshimura, Teizo; Leonard, Edward J.

In: Journal of Clinical Investigation, Vol. 86, No. 5, 11.1990, p. 1556-1564.

Research output: Contribution to journalArticle

@article{057acf834a9d46f59b234dd625ba2ba0,
title = "Macrophages cultured in vitro release leukotriene B4 and neutrophil attractant/activation protein (interleukin 8) sequentially in response to stimulation with lipopolysaccharide and zymosan",
abstract = "The capacity of lipopolysaccharide (LPS), zymosan, arid calcium ionophore A23187 to induce neutrophil chemotactic activity (NCA), leukotriene B4 (LTB4), and neutrophil attractant/activation protein (NAP-1) release from human alveolar macrophages (AM) retrieved from normal nonsmokers was evaluated. LPS induced a dose-dependent release Of LTB4 that began by 1 h, 4.0±3.2 ng/106 viable AM; peaked at 3 h, 24.7±13.5 ng/106 viable AM; and decreased by 24 h, 1.2±1.0 ng/106 viable AM (n = 8). Quantities of LTB4 in cell-free supernatants of AM stimulated with LPS were determined by reverse-phase high-performance liquid chromatography and corresponded well with results obtained by radioimmunoassay. By contrast, NAP-1 release began ∼ 3-5 h after stimulation of AM with LPS, 197±192 ng/ml, and peaked at 24 h, 790±124 ng/ml. Release of NAP-1 was stimulus specific because A23187 evoked the release of LTB4 but not NAP-1, whereas LPS and zymosan induced the release of both LTB4 and NAP-1. The appearance of neutrophil chemotactic activity in supernatants of AM challenged with LPS for 3 h could be explained completely by the quantities of LTB4 present. After stimulation with LPS or zymosan for 24 h, AM had metabolized almost all generated LTB4. Preincubation of AM with nordihydroguiaretic acid (10-4 M) completely abolished the appearance of NCA, LTB4, and NAP-1 in supernatants of AM challenged with LPS. Therefore, LPS and zymosan particles were potent stimuli of the sequential release of LTB4 and NAP-I from AM.",
keywords = "Alveolar macrophages, Interleukin 8, Leukotriene B, Lipopolysaccharide, Neutrophil attractant/activation protein, Zymosan",
author = "Rankin, {John A.} and Ilona Sylvester and Sharon Smith and Teizo Yoshimura and Leonard, {Edward J.}",
year = "1990",
month = "11",
language = "English",
volume = "86",
pages = "1556--1564",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "5",

}

TY - JOUR

T1 - Macrophages cultured in vitro release leukotriene B4 and neutrophil attractant/activation protein (interleukin 8) sequentially in response to stimulation with lipopolysaccharide and zymosan

AU - Rankin, John A.

AU - Sylvester, Ilona

AU - Smith, Sharon

AU - Yoshimura, Teizo

AU - Leonard, Edward J.

PY - 1990/11

Y1 - 1990/11

N2 - The capacity of lipopolysaccharide (LPS), zymosan, arid calcium ionophore A23187 to induce neutrophil chemotactic activity (NCA), leukotriene B4 (LTB4), and neutrophil attractant/activation protein (NAP-1) release from human alveolar macrophages (AM) retrieved from normal nonsmokers was evaluated. LPS induced a dose-dependent release Of LTB4 that began by 1 h, 4.0±3.2 ng/106 viable AM; peaked at 3 h, 24.7±13.5 ng/106 viable AM; and decreased by 24 h, 1.2±1.0 ng/106 viable AM (n = 8). Quantities of LTB4 in cell-free supernatants of AM stimulated with LPS were determined by reverse-phase high-performance liquid chromatography and corresponded well with results obtained by radioimmunoassay. By contrast, NAP-1 release began ∼ 3-5 h after stimulation of AM with LPS, 197±192 ng/ml, and peaked at 24 h, 790±124 ng/ml. Release of NAP-1 was stimulus specific because A23187 evoked the release of LTB4 but not NAP-1, whereas LPS and zymosan induced the release of both LTB4 and NAP-1. The appearance of neutrophil chemotactic activity in supernatants of AM challenged with LPS for 3 h could be explained completely by the quantities of LTB4 present. After stimulation with LPS or zymosan for 24 h, AM had metabolized almost all generated LTB4. Preincubation of AM with nordihydroguiaretic acid (10-4 M) completely abolished the appearance of NCA, LTB4, and NAP-1 in supernatants of AM challenged with LPS. Therefore, LPS and zymosan particles were potent stimuli of the sequential release of LTB4 and NAP-I from AM.

AB - The capacity of lipopolysaccharide (LPS), zymosan, arid calcium ionophore A23187 to induce neutrophil chemotactic activity (NCA), leukotriene B4 (LTB4), and neutrophil attractant/activation protein (NAP-1) release from human alveolar macrophages (AM) retrieved from normal nonsmokers was evaluated. LPS induced a dose-dependent release Of LTB4 that began by 1 h, 4.0±3.2 ng/106 viable AM; peaked at 3 h, 24.7±13.5 ng/106 viable AM; and decreased by 24 h, 1.2±1.0 ng/106 viable AM (n = 8). Quantities of LTB4 in cell-free supernatants of AM stimulated with LPS were determined by reverse-phase high-performance liquid chromatography and corresponded well with results obtained by radioimmunoassay. By contrast, NAP-1 release began ∼ 3-5 h after stimulation of AM with LPS, 197±192 ng/ml, and peaked at 24 h, 790±124 ng/ml. Release of NAP-1 was stimulus specific because A23187 evoked the release of LTB4 but not NAP-1, whereas LPS and zymosan induced the release of both LTB4 and NAP-1. The appearance of neutrophil chemotactic activity in supernatants of AM challenged with LPS for 3 h could be explained completely by the quantities of LTB4 present. After stimulation with LPS or zymosan for 24 h, AM had metabolized almost all generated LTB4. Preincubation of AM with nordihydroguiaretic acid (10-4 M) completely abolished the appearance of NCA, LTB4, and NAP-1 in supernatants of AM challenged with LPS. Therefore, LPS and zymosan particles were potent stimuli of the sequential release of LTB4 and NAP-I from AM.

KW - Alveolar macrophages

KW - Interleukin 8

KW - Leukotriene B

KW - Lipopolysaccharide

KW - Neutrophil attractant/activation protein

KW - Zymosan

UR - http://www.scopus.com/inward/record.url?scp=0025016233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025016233&partnerID=8YFLogxK

M3 - Article

C2 - 2173722

AN - SCOPUS:0025016233

VL - 86

SP - 1556

EP - 1564

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 5

ER -