Macrophage Migration Inhibitory Factor Deficiency Causes Prolonged Hearing Loss After Acoustic Overstimulation

Shin Kariya, Mitsuhiro Okano, Yukihide Maeda, Haruka Hirai, Takaya Higaki, Yasuyuki Noyama, Takenori Haruna, Jun Nishihira, Kazunori Nishizaki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Hypothesis: Macrophage migration inhibitory factor plays an important role in noise-induced hearing loss. Background: Macrophage migration inhibitory factor is an essential factor in axis formation and neural development. Macrophage migration inhibitory factor is expressed in the inner ear, but its function remains to be elucidated. Methods: Macrophage migration inhibitory factor-deficient mice (MIF-/- mice) were used in this study. Wild-type and MIF-/- mice received noise exposure composed of octave band noise. Auditory brainstem response thresholds were examined before (control) and at 0, 12, and 24 hours and 2 weeks after the intense noise exposure. Morphological findings of cochlear hair cells were investigated using scanning electron microscopy. Histopathological examination with hematoxylin and eosin staining and TUNEL assay were also performed. Results: In both the wild-type and MIF-/- mice, acoustic overstimulation induced significant hearing loss compared with the control level. Two weeks after the intense noise exposure, the MIF-/- mice had an increased hearing threshold compared with the wild-type mice. Scanning electron microscopy demonstrated that the outer hair cells in the MIF-/- mice were affected 2 weeks after noise exposure compared with the wild-type mice. TUNEL-positive cells were identified in the organ of Corti of the MIF-/- mice. Conclusion: The MIF-/- mice had prolonged hearing loss and significant loss of cochlear hair cells after intense noise exposure. Macrophage migration inhibitory factor may play an important role in recovery from acoustic trauma. Management of macrophage migration inhibitory factor may be a novel therapeutic option for noise-induced hearing loss.

Original languageEnglish
Pages (from-to)1103-1108
Number of pages6
JournalOtology and Neurotology
Volume36
Issue number6
DOIs
Publication statusPublished - Jul 26 2015

Fingerprint

Macrophage Migration-Inhibitory Factors
Hearing Loss
Acoustics
Noise
Noise-Induced Hearing Loss
Auditory Hair Cells
In Situ Nick-End Labeling
Outer Auditory Hair Cells
Electron Scanning Microscopy
Organ of Corti
Brain Stem Auditory Evoked Potentials
Inner Ear
Hematoxylin
Eosine Yellowish-(YS)
Hearing
Cell Movement
Staining and Labeling

Keywords

  • Cochlea
  • Ear
  • Hearing loss
  • Macrophage migration inhibitory factor
  • Noise-induced hearing loss

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Clinical Neurology
  • Sensory Systems

Cite this

Macrophage Migration Inhibitory Factor Deficiency Causes Prolonged Hearing Loss After Acoustic Overstimulation. / Kariya, Shin; Okano, Mitsuhiro; Maeda, Yukihide; Hirai, Haruka; Higaki, Takaya; Noyama, Yasuyuki; Haruna, Takenori; Nishihira, Jun; Nishizaki, Kazunori.

In: Otology and Neurotology, Vol. 36, No. 6, 26.07.2015, p. 1103-1108.

Research output: Contribution to journalArticle

Kariya, Shin ; Okano, Mitsuhiro ; Maeda, Yukihide ; Hirai, Haruka ; Higaki, Takaya ; Noyama, Yasuyuki ; Haruna, Takenori ; Nishihira, Jun ; Nishizaki, Kazunori. / Macrophage Migration Inhibitory Factor Deficiency Causes Prolonged Hearing Loss After Acoustic Overstimulation. In: Otology and Neurotology. 2015 ; Vol. 36, No. 6. pp. 1103-1108.
@article{ecf4bd2c067d4fb281a6d55df0f2466e,
title = "Macrophage Migration Inhibitory Factor Deficiency Causes Prolonged Hearing Loss After Acoustic Overstimulation",
abstract = "Hypothesis: Macrophage migration inhibitory factor plays an important role in noise-induced hearing loss. Background: Macrophage migration inhibitory factor is an essential factor in axis formation and neural development. Macrophage migration inhibitory factor is expressed in the inner ear, but its function remains to be elucidated. Methods: Macrophage migration inhibitory factor-deficient mice (MIF-/- mice) were used in this study. Wild-type and MIF-/- mice received noise exposure composed of octave band noise. Auditory brainstem response thresholds were examined before (control) and at 0, 12, and 24 hours and 2 weeks after the intense noise exposure. Morphological findings of cochlear hair cells were investigated using scanning electron microscopy. Histopathological examination with hematoxylin and eosin staining and TUNEL assay were also performed. Results: In both the wild-type and MIF-/- mice, acoustic overstimulation induced significant hearing loss compared with the control level. Two weeks after the intense noise exposure, the MIF-/- mice had an increased hearing threshold compared with the wild-type mice. Scanning electron microscopy demonstrated that the outer hair cells in the MIF-/- mice were affected 2 weeks after noise exposure compared with the wild-type mice. TUNEL-positive cells were identified in the organ of Corti of the MIF-/- mice. Conclusion: The MIF-/- mice had prolonged hearing loss and significant loss of cochlear hair cells after intense noise exposure. Macrophage migration inhibitory factor may play an important role in recovery from acoustic trauma. Management of macrophage migration inhibitory factor may be a novel therapeutic option for noise-induced hearing loss.",
keywords = "Cochlea, Ear, Hearing loss, Macrophage migration inhibitory factor, Noise-induced hearing loss",
author = "Shin Kariya and Mitsuhiro Okano and Yukihide Maeda and Haruka Hirai and Takaya Higaki and Yasuyuki Noyama and Takenori Haruna and Jun Nishihira and Kazunori Nishizaki",
year = "2015",
month = "7",
day = "26",
doi = "10.1097/MAO.0000000000000755",
language = "English",
volume = "36",
pages = "1103--1108",
journal = "Otology and Neurotology",
issn = "1531-7129",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Macrophage Migration Inhibitory Factor Deficiency Causes Prolonged Hearing Loss After Acoustic Overstimulation

AU - Kariya, Shin

AU - Okano, Mitsuhiro

AU - Maeda, Yukihide

AU - Hirai, Haruka

AU - Higaki, Takaya

AU - Noyama, Yasuyuki

AU - Haruna, Takenori

AU - Nishihira, Jun

AU - Nishizaki, Kazunori

PY - 2015/7/26

Y1 - 2015/7/26

N2 - Hypothesis: Macrophage migration inhibitory factor plays an important role in noise-induced hearing loss. Background: Macrophage migration inhibitory factor is an essential factor in axis formation and neural development. Macrophage migration inhibitory factor is expressed in the inner ear, but its function remains to be elucidated. Methods: Macrophage migration inhibitory factor-deficient mice (MIF-/- mice) were used in this study. Wild-type and MIF-/- mice received noise exposure composed of octave band noise. Auditory brainstem response thresholds were examined before (control) and at 0, 12, and 24 hours and 2 weeks after the intense noise exposure. Morphological findings of cochlear hair cells were investigated using scanning electron microscopy. Histopathological examination with hematoxylin and eosin staining and TUNEL assay were also performed. Results: In both the wild-type and MIF-/- mice, acoustic overstimulation induced significant hearing loss compared with the control level. Two weeks after the intense noise exposure, the MIF-/- mice had an increased hearing threshold compared with the wild-type mice. Scanning electron microscopy demonstrated that the outer hair cells in the MIF-/- mice were affected 2 weeks after noise exposure compared with the wild-type mice. TUNEL-positive cells were identified in the organ of Corti of the MIF-/- mice. Conclusion: The MIF-/- mice had prolonged hearing loss and significant loss of cochlear hair cells after intense noise exposure. Macrophage migration inhibitory factor may play an important role in recovery from acoustic trauma. Management of macrophage migration inhibitory factor may be a novel therapeutic option for noise-induced hearing loss.

AB - Hypothesis: Macrophage migration inhibitory factor plays an important role in noise-induced hearing loss. Background: Macrophage migration inhibitory factor is an essential factor in axis formation and neural development. Macrophage migration inhibitory factor is expressed in the inner ear, but its function remains to be elucidated. Methods: Macrophage migration inhibitory factor-deficient mice (MIF-/- mice) were used in this study. Wild-type and MIF-/- mice received noise exposure composed of octave band noise. Auditory brainstem response thresholds were examined before (control) and at 0, 12, and 24 hours and 2 weeks after the intense noise exposure. Morphological findings of cochlear hair cells were investigated using scanning electron microscopy. Histopathological examination with hematoxylin and eosin staining and TUNEL assay were also performed. Results: In both the wild-type and MIF-/- mice, acoustic overstimulation induced significant hearing loss compared with the control level. Two weeks after the intense noise exposure, the MIF-/- mice had an increased hearing threshold compared with the wild-type mice. Scanning electron microscopy demonstrated that the outer hair cells in the MIF-/- mice were affected 2 weeks after noise exposure compared with the wild-type mice. TUNEL-positive cells were identified in the organ of Corti of the MIF-/- mice. Conclusion: The MIF-/- mice had prolonged hearing loss and significant loss of cochlear hair cells after intense noise exposure. Macrophage migration inhibitory factor may play an important role in recovery from acoustic trauma. Management of macrophage migration inhibitory factor may be a novel therapeutic option for noise-induced hearing loss.

KW - Cochlea

KW - Ear

KW - Hearing loss

KW - Macrophage migration inhibitory factor

KW - Noise-induced hearing loss

UR - http://www.scopus.com/inward/record.url?scp=84933505886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84933505886&partnerID=8YFLogxK

U2 - 10.1097/MAO.0000000000000755

DO - 10.1097/MAO.0000000000000755

M3 - Article

C2 - 25853607

AN - SCOPUS:84933505886

VL - 36

SP - 1103

EP - 1108

JO - Otology and Neurotology

JF - Otology and Neurotology

SN - 1531-7129

IS - 6

ER -