M-RIP, a novel target of JNK signaling and a requirement for human cancer cell invasion

Ryoko Ono, Junji Matsuoka, Tomoki Yamatsuji, Yoshio Naomoto, Noriaki Tanaka, Hideki Matsui, Masayuki Matsushita

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cell motility is involved in physiological and pathological processes such as the invasion and migration of cells. c-Jun N-terminal kinase (JNK) cascades are involved in the invasion and metastasis of cancer cells. However, little is known about the downstream signaling of JNK. In the present study, we used small interfering RNA (siRNA) directed against JNK1 to reduce its expression. We used microarray techniques to compare the gene expression profiles of epidermal growth factor (EGF)-stimulated HeLa cells with and without JNK1 siRNA treatment. We identified a JNK1 target gene, myosin phosphatase-Rho interacting protein (M-RIP). RNA interference-mediated inhibition of JNK1 strongly inhibited M-RIP mRNA expression induced by EGF, as well as the invasion of HeLa cells. In addition, M-RIP siRNA-treated cells showed significantly reduced invasive activity. Thus, a functional analysis of JNK1 and M-RIP with RNA interference reveals a critical role for this cascade in the invasive behavior of cancer cells.

Original languageEnglish
Pages (from-to)199-203
Number of pages5
JournalInternational journal of molecular medicine
Volume22
Issue number2
DOIs
Publication statusPublished - Aug 1 2008

Keywords

  • Eukaryotic initiation factor
  • Focal adhesion kinase
  • Jun
  • Myosin phosphatase-Rho interacting protein
  • Small interfering RNA

ASJC Scopus subject areas

  • Genetics

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