Lysophosphatidic Acid Synthesis and its Receptors’ Expression in the Bovine Oviduct During the Oestrous Cycle

E. Sinderewicz, K. Grycmacher, D. Boruszewska, I. Kowalczyk-Zięba, Yuki Yamamoto, Y. Yoshimoto, I. Woclawek-Potocka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lysophosphatidic acid (LPA) is a naturally occurring simple phospholipid which in the bovine reproductive system can be produced in the endometrium, corpus luteum, ovarian follicle and embryo. In this study, we examined the possibility that LPA receptors are expressed, and LPA synthesized, in the bovine oviduct. We found that the concentration of LPA was highest in infundibulum in the follicular phase of the oestrous cycle and was relatively high during the early-luteal phase in all examined parts of the oviduct. We also documented that LPA synthesis engages both available pathways for LPA production. The autotaxin (ATX) protein expression was significantly higher in the infundibulum compared to the isthmus during the follicular phase of the oestrous cycle. During the early-luteal phase of the oestrous cycle, ATX and phospholipase A2 (PLA2) protein expression was highest in ampulla, although the expression of LPARs was not as dynamic as LPA concentration in the oviduct tissue, and we presume that in the bovine oviduct, the most abundantly expressed receptor is LPAR2. In conclusion, our results indicate that the bovine oviduct is a site of LPA synthesis and a target for LPA action in the bovine reproductive tract. We documented that LPAR2 is the most abundantly expressed in the bovine oviduct. We hypothesize that in the bovine oviduct, LPA may be involved in the transport of gametes, fertilization and cellular signalling between the oviduct and cumulus–oocyte complex.

Original languageEnglish
Pages (from-to)541-549
Number of pages9
JournalReproduction in Domestic Animals
Volume51
Issue number4
DOIs
Publication statusPublished - Aug 1 2016

Fingerprint

Oviducts
oviducts
estrous cycle
receptors
synthesis
acids
cattle
corpus luteum
Follicular Phase
Luteal Phase
Pituitary Gland
Lysophosphatidic Acid Receptors
protein synthesis
lysophosphatidic acid
accessory sex glands
Ovarian Follicle
Phospholipases A2
Corpus Luteum
cell communication
phospholipase A2

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Endocrinology

Cite this

Sinderewicz, E., Grycmacher, K., Boruszewska, D., Kowalczyk-Zięba, I., Yamamoto, Y., Yoshimoto, Y., & Woclawek-Potocka, I. (2016). Lysophosphatidic Acid Synthesis and its Receptors’ Expression in the Bovine Oviduct During the Oestrous Cycle. Reproduction in Domestic Animals, 51(4), 541-549. https://doi.org/10.1111/rda.12717

Lysophosphatidic Acid Synthesis and its Receptors’ Expression in the Bovine Oviduct During the Oestrous Cycle. / Sinderewicz, E.; Grycmacher, K.; Boruszewska, D.; Kowalczyk-Zięba, I.; Yamamoto, Yuki; Yoshimoto, Y.; Woclawek-Potocka, I.

In: Reproduction in Domestic Animals, Vol. 51, No. 4, 01.08.2016, p. 541-549.

Research output: Contribution to journalArticle

Sinderewicz, E, Grycmacher, K, Boruszewska, D, Kowalczyk-Zięba, I, Yamamoto, Y, Yoshimoto, Y & Woclawek-Potocka, I 2016, 'Lysophosphatidic Acid Synthesis and its Receptors’ Expression in the Bovine Oviduct During the Oestrous Cycle', Reproduction in Domestic Animals, vol. 51, no. 4, pp. 541-549. https://doi.org/10.1111/rda.12717
Sinderewicz, E. ; Grycmacher, K. ; Boruszewska, D. ; Kowalczyk-Zięba, I. ; Yamamoto, Yuki ; Yoshimoto, Y. ; Woclawek-Potocka, I. / Lysophosphatidic Acid Synthesis and its Receptors’ Expression in the Bovine Oviduct During the Oestrous Cycle. In: Reproduction in Domestic Animals. 2016 ; Vol. 51, No. 4. pp. 541-549.
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