Abstract
Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase (PL)D2 and PLA2G1B) inthe bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPAR1) was positively correlated with the expression of PGE2 synthase (PGES) and negatively correlated with the expression of PGF2α synthase (aldose reductasewith20 α-hydroxysteroid dehydrogenase activity - PGFS) during early pregnancy. In vivo LPA induced P4 and PGE2 secretionwas inhibited by LPAR1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover, LPAR1 gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracrine roles in the bovine uterus. LPAR1 gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE2 production (PGES) in endometrium. In cow, LPA stimulates P4 and PGE2 secretion. Thus, LPA in the bovine reproductive tractmay indirectly (via endometrium) or directly support corpus luteumaction via the increase of P4 synthesis and the increase of PGE2/PGF2α ratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow.
| Original language | English |
|---|---|
| Pages (from-to) | 95-105 |
| Number of pages | 11 |
| Journal | Reproduction |
| Volume | 137 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 |
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ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Reproductive Medicine
- Embryology
- Endocrinology
- Cell Biology
Cite this
Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus. / Woclawek-Potocka, Izabela; Komiyama, Junichi; Saulnier-Blache, Jean Sebastian; Brzezicka, Edyta; Bah, Mamadou Moussa; Okuda, Kiyoshi; Skarzynski, Dariusz J.
In: Reproduction, Vol. 137, No. 1, 2009, p. 95-105.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus
AU - Woclawek-Potocka, Izabela
AU - Komiyama, Junichi
AU - Saulnier-Blache, Jean Sebastian
AU - Brzezicka, Edyta
AU - Bah, Mamadou Moussa
AU - Okuda, Kiyoshi
AU - Skarzynski, Dariusz J.
PY - 2009
Y1 - 2009
N2 - Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase (PL)D2 and PLA2G1B) inthe bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPAR1) was positively correlated with the expression of PGE2 synthase (PGES) and negatively correlated with the expression of PGF2α synthase (aldose reductasewith20 α-hydroxysteroid dehydrogenase activity - PGFS) during early pregnancy. In vivo LPA induced P4 and PGE2 secretionwas inhibited by LPAR1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover, LPAR1 gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracrine roles in the bovine uterus. LPAR1 gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE2 production (PGES) in endometrium. In cow, LPA stimulates P4 and PGE2 secretion. Thus, LPA in the bovine reproductive tractmay indirectly (via endometrium) or directly support corpus luteumaction via the increase of P4 synthesis and the increase of PGE2/PGF2α ratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow.
AB - Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase (PL)D2 and PLA2G1B) inthe bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPAR1) was positively correlated with the expression of PGE2 synthase (PGES) and negatively correlated with the expression of PGF2α synthase (aldose reductasewith20 α-hydroxysteroid dehydrogenase activity - PGFS) during early pregnancy. In vivo LPA induced P4 and PGE2 secretionwas inhibited by LPAR1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover, LPAR1 gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracrine roles in the bovine uterus. LPAR1 gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE2 production (PGES) in endometrium. In cow, LPA stimulates P4 and PGE2 secretion. Thus, LPA in the bovine reproductive tractmay indirectly (via endometrium) or directly support corpus luteumaction via the increase of P4 synthesis and the increase of PGE2/PGF2α ratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow.
UR - http://www.scopus.com/inward/record.url?scp=65949123347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65949123347&partnerID=8YFLogxK
U2 - 10.1530/REP-08-0209
DO - 10.1530/REP-08-0209
M3 - Article
C2 - 18829944
AN - SCOPUS:65949123347
VL - 137
SP - 95
EP - 105
JO - Reproduction
JF - Reproduction
SN - 1470-1626
IS - 1
ER -