Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus

Izabela Woclawek-Potocka, Junichi Komiyama, Jean Sebastian Saulnier-Blache, Edyta Brzezicka, Mamadou Moussa Bah, Kiyoshi Okuda, Dariusz J. Skarzynski

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Abstract

Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase (PL)D2 and PLA2G1B) inthe bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPAR1) was positively correlated with the expression of PGE2 synthase (PGES) and negatively correlated with the expression of PGF synthase (aldose reductasewith20 α-hydroxysteroid dehydrogenase activity - PGFS) during early pregnancy. In vivo LPA induced P4 and PGE2 secretionwas inhibited by LPAR1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover, LPAR1 gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracrine roles in the bovine uterus. LPAR1 gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE2 production (PGES) in endometrium. In cow, LPA stimulates P4 and PGE2 secretion. Thus, LPA in the bovine reproductive tractmay indirectly (via endometrium) or directly support corpus luteumaction via the increase of P4 synthesis and the increase of PGE2/PGF ratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow.

Original languageEnglish
Pages (from-to)95-105
Number of pages11
JournalReproduction
Volume137
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

Uterus
Prostaglandins
Endometrium
Acids
Lysophosphatidic Acid Receptors
Dinoprostone
Dinoprost
Estrous Cycle
Pregnancy
lysophosphatidic acid
Pregnancy Maintenance
Gene Expression
Hydroxysteroid Dehydrogenases
Enzymes
Embryonic Structures
Mortality

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Cite this

Woclawek-Potocka, I., Komiyama, J., Saulnier-Blache, J. S., Brzezicka, E., Bah, M. M., Okuda, K., & Skarzynski, D. J. (2009). Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus. Reproduction, 137(1), 95-105. https://doi.org/10.1530/REP-08-0209

Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus. / Woclawek-Potocka, Izabela; Komiyama, Junichi; Saulnier-Blache, Jean Sebastian; Brzezicka, Edyta; Bah, Mamadou Moussa; Okuda, Kiyoshi; Skarzynski, Dariusz J.

In: Reproduction, Vol. 137, No. 1, 2009, p. 95-105.

Research output: Contribution to journalArticle

Woclawek-Potocka, I, Komiyama, J, Saulnier-Blache, JS, Brzezicka, E, Bah, MM, Okuda, K & Skarzynski, DJ 2009, 'Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus', Reproduction, vol. 137, no. 1, pp. 95-105. https://doi.org/10.1530/REP-08-0209
Woclawek-Potocka I, Komiyama J, Saulnier-Blache JS, Brzezicka E, Bah MM, Okuda K et al. Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus. Reproduction. 2009;137(1):95-105. https://doi.org/10.1530/REP-08-0209
Woclawek-Potocka, Izabela ; Komiyama, Junichi ; Saulnier-Blache, Jean Sebastian ; Brzezicka, Edyta ; Bah, Mamadou Moussa ; Okuda, Kiyoshi ; Skarzynski, Dariusz J. / Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus. In: Reproduction. 2009 ; Vol. 137, No. 1. pp. 95-105.
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