Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus

Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase (PL)D₂ and PLA2G1B) inthe bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPAR1) was positively correlated with the expression of PGE₂ synthase (PGES) and negatively correlated with the expression of PGF_2α synthase (aldose reductasewith20 α-hydroxysteroid dehydrogenase activity - PGFS) during early pregnancy. In vivo LPA induced P4 and PGE₂ secretionwas inhibited by LPAR1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover, LPAR1 gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracrine roles in the bovine uterus. LPAR1 gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE₂ production (PGES) in endometrium. In cow, LPA stimulates P4 and PGE₂ secretion. Thus, LPA in the bovine reproductive tractmay indirectly (via endometrium) or directly support corpus luteumaction via the increase of P4 synthesis and the increase of PGE₂/PGF_2α ratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow.