Lysine-mediated tissue osmication in combination with a tannin-osmium conductive staining method for non-coated scanning electron microscopy of biological specimens.

T. Murakami, Z. L. Song, H. Hinenoya, Aiji Ohtsuka, Takehito Taguchi, J. J. Liu, T. Sano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5-30 kV and a specimen current of 1 X 10(-10) A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutaraldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).

Original languageEnglish
Pages (from-to)485-493
Number of pages9
JournalArchivum Histologicum Japonicum
Volume50
Issue number5
Publication statusPublished - Dec 1987

Fingerprint

Osmium
Tannins
Electron Scanning Microscopy
Lysine
Glutaral
Staining and Labeling
Electrons
Osmium Tetroxide
Podocytes
Gold
Metals
Kidney

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Lysine-mediated tissue osmication in combination with a tannin-osmium conductive staining method for non-coated scanning electron microscopy of biological specimens. / Murakami, T.; Song, Z. L.; Hinenoya, H.; Ohtsuka, Aiji; Taguchi, Takehito; Liu, J. J.; Sano, T.

In: Archivum Histologicum Japonicum, Vol. 50, No. 5, 12.1987, p. 485-493.

Research output: Contribution to journalArticle

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