Lysine-Mediated Tissue Osmication in Combination with a Tannin-Osmium Conductive Staining Method for Non-Coated Scanning Electron Microscopy of Biological Specimens

Takuro Murakami, Zhen lan Song, Hitoshi Hinenoya, Aiji Ohtsuka, Takehito Taguch, Jing jie Liu, Tadashi Sano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5–30 kV and a specimen current of 1 x 10-10A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutar-aldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).

Original languageEnglish
Pages (from-to)485-493
Number of pages9
JournalArchivum histologicum japonicum
Volume50
Issue number5
DOIs
Publication statusPublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Anatomy
  • Histology

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