Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes

Toru Ide, Takaaki Aoki, Yuko Takeuchi, Toshio Yanagida

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Lysenin, a hemolytic protein derived from the body fluid of earthworm, was incorporated into artificial bilayer membranes. Upon insertion, it formed a voltage-dependent large conductance channel in asolectin bilayers in a sphingomyelin-dependent manner. The channel had low ion-selectivity. Single-channel conductance was calculated as approximately 550 pS in 100 mM KCl. The channel in asolectin bilayers closed when the membrane was held at a positive potential. In contrast, the channel showed no voltage dependency in membranes made of pure phosphatidylcholine and sphingomyelin, suggesting some lipid contents included in the asolectin membranes affected channel gating.

Original languageEnglish
Pages (from-to)288-292
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume346
Issue number1
DOIs
Publication statusPublished - Jul 21 2006
Externally publishedYes

Fingerprint

Lipid bilayers
Lipid Bilayers
Membrane Lipids
Sphingomyelins
Membranes
Electric potential
Artificial Membranes
Oligochaeta
Body fluids
Body Fluids
Phosphatidylcholines
Ion Channels
Ions
Lipids
lysenin
asolectin
Proteins

Keywords

  • Lysenin
  • Planar bilayer
  • Single-channel
  • Sphingomyelin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes. / Ide, Toru; Aoki, Takaaki; Takeuchi, Yuko; Yanagida, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 346, No. 1, 21.07.2006, p. 288-292.

Research output: Contribution to journalArticle

Ide, Toru ; Aoki, Takaaki ; Takeuchi, Yuko ; Yanagida, Toshio. / Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 346, No. 1. pp. 288-292.
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