Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome

Paul R.J. Ames, C. Tommasino, G. Fossati, E. Matsuura, A. Margarita, A. Saulino, L. Lopez, G. Scenna, V. Brancaccio

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    7 Citations (Scopus)

    Abstract

    The aim of this study was to evaluate a possible association between lymphocyte subsets and intima media thickness (IMT) of carotid arteries in primary antiphospholipid syndrome (PAPS). We used a cross-sectional study on PAPS patients (n = 18) and healthy controls (n = 16). IgG anti-cardiolipin antibody (aCL), IgG anti-β2glycoprotein-I (anti- β2GPI), IgG anti-β2glycoprotein-I complexed to oxidized low-density lipoprotein (oxLDL) and to a specific oxidized moiety of LDL (oxLig1), and β2GPI-oxLDL were measured by ELISA. Lymphocyte immunophenotyping was performed using pairs of monoclonal antibodies directly labelled with fluorescein isothiocyanate, or phycoerythrin or phycoerythrin-Texas-red-X. Intima media thickness (IMT) of carotid arteries was determined by high-resolution sonography. Total peripheral blood lymphocytes did not differ between PAPS and controls. Memory CD4+/CD45RO + T cells were lower in PAPS than controls (P = 0.0007) as well as CD16+56+ natural killer cells (P = 0.02). In PAPS memory T CD45RO + cells positively correlated with IgG anti-β2GPI-oxLig1 (P = 0.002) and to IMT of carotid arteries (common carotid P = 0.02, bifurcation P = 0.007). Naïve CD4+/CD45RA+ T cells inversely correlated with β2GPI-oxLDL (P = 0.009). The relation between IgG anti-β2GPI-oxLig1 and IMT of carotid arteries with memory CD45RO + T lymphocytes suggests a role for the latter in PAPS related atherogenesis.

    Original languageEnglish
    Pages (from-to)809-813
    Number of pages5
    JournalLupus
    Volume14
    Issue number10
    DOIs
    Publication statusPublished - 2005

    Keywords

    • Antiphospholipid antibodies
    • Atherosclerosis
    • IgG anti-β2GPI-oxLig1
    • Lymphocytes

    ASJC Scopus subject areas

    • Rheumatology

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