Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome

Paul R J Ames, C. Tommasino, G. Fossati, Eiji Matsuura, A. Margarita, A. Saulino, L. Lopez, G. Scenna, V. Brancaccio

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The aim of this study was to evaluate a possible association between lymphocyte subsets and intima media thickness (IMT) of carotid arteries in primary antiphospholipid syndrome (PAPS). We used a cross-sectional study on PAPS patients (n = 18) and healthy controls (n = 16). IgG anti-cardiolipin antibody (aCL), IgG anti-β2glycoprotein-I (anti- β2GPI), IgG anti-β2glycoprotein-I complexed to oxidized low-density lipoprotein (oxLDL) and to a specific oxidized moiety of LDL (oxLig1), and β2GPI-oxLDL were measured by ELISA. Lymphocyte immunophenotyping was performed using pairs of monoclonal antibodies directly labelled with fluorescein isothiocyanate, or phycoerythrin or phycoerythrin-Texas-red-X. Intima media thickness (IMT) of carotid arteries was determined by high-resolution sonography. Total peripheral blood lymphocytes did not differ between PAPS and controls. Memory CD4+/CD45RO + T cells were lower in PAPS than controls (P = 0.0007) as well as CD16+56+ natural killer cells (P = 0.02). In PAPS memory T CD45RO + cells positively correlated with IgG anti-β2GPI-oxLig1 (P = 0.002) and to IMT of carotid arteries (common carotid P = 0.02, bifurcation P = 0.007). Naïve CD4+/CD45RA+ T cells inversely correlated with β2GPI-oxLDL (P = 0.009). The relation between IgG anti-β2GPI-oxLig1 and IMT of carotid arteries with memory CD45RO + T lymphocytes suggests a role for the latter in PAPS related atherogenesis.

Original languageEnglish
Pages (from-to)809-813
Number of pages5
JournalLupus
Volume14
Issue number10
DOIs
Publication statusPublished - 2005

Fingerprint

Antiphospholipid Syndrome
Lymphocyte Subsets
Carotid Arteries
Phycoerythrin
Common Carotid Artery
T-Lymphocytes
Immunophenotyping
Carotid Intima-Media Thickness
Cardiolipins
Fluorescein
Natural Killer Cells
Anti-Idiotypic Antibodies
Ultrasonography
Atherosclerosis
Cross-Sectional Studies
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Lymphocytes
anti-IgG

Keywords

  • Antiphospholipid antibodies
  • Atherosclerosis
  • IgG anti-β2GPI-oxLig1
  • Lymphocytes

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Ames, P. R. J., Tommasino, C., Fossati, G., Matsuura, E., Margarita, A., Saulino, A., ... Brancaccio, V. (2005). Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome. Lupus, 14(10), 809-813. https://doi.org/10.1191/0961203305lu2181oa

Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome. / Ames, Paul R J; Tommasino, C.; Fossati, G.; Matsuura, Eiji; Margarita, A.; Saulino, A.; Lopez, L.; Scenna, G.; Brancaccio, V.

In: Lupus, Vol. 14, No. 10, 2005, p. 809-813.

Research output: Contribution to journalArticle

Ames, PRJ, Tommasino, C, Fossati, G, Matsuura, E, Margarita, A, Saulino, A, Lopez, L, Scenna, G & Brancaccio, V 2005, 'Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome', Lupus, vol. 14, no. 10, pp. 809-813. https://doi.org/10.1191/0961203305lu2181oa
Ames, Paul R J ; Tommasino, C. ; Fossati, G. ; Matsuura, Eiji ; Margarita, A. ; Saulino, A. ; Lopez, L. ; Scenna, G. ; Brancaccio, V. / Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome. In: Lupus. 2005 ; Vol. 14, No. 10. pp. 809-813.
@article{685ebd72fc2e4f7fa14884b780f1ec5f,
title = "Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome",
abstract = "The aim of this study was to evaluate a possible association between lymphocyte subsets and intima media thickness (IMT) of carotid arteries in primary antiphospholipid syndrome (PAPS). We used a cross-sectional study on PAPS patients (n = 18) and healthy controls (n = 16). IgG anti-cardiolipin antibody (aCL), IgG anti-β2glycoprotein-I (anti- β2GPI), IgG anti-β2glycoprotein-I complexed to oxidized low-density lipoprotein (oxLDL) and to a specific oxidized moiety of LDL (oxLig1), and β2GPI-oxLDL were measured by ELISA. Lymphocyte immunophenotyping was performed using pairs of monoclonal antibodies directly labelled with fluorescein isothiocyanate, or phycoerythrin or phycoerythrin-Texas-red-X. Intima media thickness (IMT) of carotid arteries was determined by high-resolution sonography. Total peripheral blood lymphocytes did not differ between PAPS and controls. Memory CD4+/CD45RO + T cells were lower in PAPS than controls (P = 0.0007) as well as CD16+56+ natural killer cells (P = 0.02). In PAPS memory T CD45RO + cells positively correlated with IgG anti-β2GPI-oxLig1 (P = 0.002) and to IMT of carotid arteries (common carotid P = 0.02, bifurcation P = 0.007). Na{\"i}ve CD4+/CD45RA+ T cells inversely correlated with β2GPI-oxLDL (P = 0.009). The relation between IgG anti-β2GPI-oxLig1 and IMT of carotid arteries with memory CD45RO + T lymphocytes suggests a role for the latter in PAPS related atherogenesis.",
keywords = "Antiphospholipid antibodies, Atherosclerosis, IgG anti-β2GPI-oxLig1, Lymphocytes",
author = "Ames, {Paul R J} and C. Tommasino and G. Fossati and Eiji Matsuura and A. Margarita and A. Saulino and L. Lopez and G. Scenna and V. Brancaccio",
year = "2005",
doi = "10.1191/0961203305lu2181oa",
language = "English",
volume = "14",
pages = "809--813",
journal = "Lupus",
issn = "0961-2033",
publisher = "SAGE Publications Ltd",
number = "10",

}

TY - JOUR

T1 - Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome

AU - Ames, Paul R J

AU - Tommasino, C.

AU - Fossati, G.

AU - Matsuura, Eiji

AU - Margarita, A.

AU - Saulino, A.

AU - Lopez, L.

AU - Scenna, G.

AU - Brancaccio, V.

PY - 2005

Y1 - 2005

N2 - The aim of this study was to evaluate a possible association between lymphocyte subsets and intima media thickness (IMT) of carotid arteries in primary antiphospholipid syndrome (PAPS). We used a cross-sectional study on PAPS patients (n = 18) and healthy controls (n = 16). IgG anti-cardiolipin antibody (aCL), IgG anti-β2glycoprotein-I (anti- β2GPI), IgG anti-β2glycoprotein-I complexed to oxidized low-density lipoprotein (oxLDL) and to a specific oxidized moiety of LDL (oxLig1), and β2GPI-oxLDL were measured by ELISA. Lymphocyte immunophenotyping was performed using pairs of monoclonal antibodies directly labelled with fluorescein isothiocyanate, or phycoerythrin or phycoerythrin-Texas-red-X. Intima media thickness (IMT) of carotid arteries was determined by high-resolution sonography. Total peripheral blood lymphocytes did not differ between PAPS and controls. Memory CD4+/CD45RO + T cells were lower in PAPS than controls (P = 0.0007) as well as CD16+56+ natural killer cells (P = 0.02). In PAPS memory T CD45RO + cells positively correlated with IgG anti-β2GPI-oxLig1 (P = 0.002) and to IMT of carotid arteries (common carotid P = 0.02, bifurcation P = 0.007). Naïve CD4+/CD45RA+ T cells inversely correlated with β2GPI-oxLDL (P = 0.009). The relation between IgG anti-β2GPI-oxLig1 and IMT of carotid arteries with memory CD45RO + T lymphocytes suggests a role for the latter in PAPS related atherogenesis.

AB - The aim of this study was to evaluate a possible association between lymphocyte subsets and intima media thickness (IMT) of carotid arteries in primary antiphospholipid syndrome (PAPS). We used a cross-sectional study on PAPS patients (n = 18) and healthy controls (n = 16). IgG anti-cardiolipin antibody (aCL), IgG anti-β2glycoprotein-I (anti- β2GPI), IgG anti-β2glycoprotein-I complexed to oxidized low-density lipoprotein (oxLDL) and to a specific oxidized moiety of LDL (oxLig1), and β2GPI-oxLDL were measured by ELISA. Lymphocyte immunophenotyping was performed using pairs of monoclonal antibodies directly labelled with fluorescein isothiocyanate, or phycoerythrin or phycoerythrin-Texas-red-X. Intima media thickness (IMT) of carotid arteries was determined by high-resolution sonography. Total peripheral blood lymphocytes did not differ between PAPS and controls. Memory CD4+/CD45RO + T cells were lower in PAPS than controls (P = 0.0007) as well as CD16+56+ natural killer cells (P = 0.02). In PAPS memory T CD45RO + cells positively correlated with IgG anti-β2GPI-oxLig1 (P = 0.002) and to IMT of carotid arteries (common carotid P = 0.02, bifurcation P = 0.007). Naïve CD4+/CD45RA+ T cells inversely correlated with β2GPI-oxLDL (P = 0.009). The relation between IgG anti-β2GPI-oxLig1 and IMT of carotid arteries with memory CD45RO + T lymphocytes suggests a role for the latter in PAPS related atherogenesis.

KW - Antiphospholipid antibodies

KW - Atherosclerosis

KW - IgG anti-β2GPI-oxLig1

KW - Lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=27744438197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744438197&partnerID=8YFLogxK

U2 - 10.1191/0961203305lu2181oa

DO - 10.1191/0961203305lu2181oa

M3 - Article

C2 - 16302675

AN - SCOPUS:27744438197

VL - 14

SP - 809

EP - 813

JO - Lupus

JF - Lupus

SN - 0961-2033

IS - 10

ER -