Abstract
Lycorine, an alkaloid extracted from Amaryllidaceae genera, exhibits antitumor activities against several human solid-tumor and leukemia cells with extensive influence on various cell signaling molecules. However, the effect of lycorine on bladder cancer has not yet been investigated. In this study, we demonstrated that lycorine induced apoptosis in human bladder cancer T24 cells, an effect that is mediated via inhibition of phospho-Akt expression and the consequent activation of caspase-3 and Bax in vitro. In an in vivo experiment, T24 cells were subcutaneously implanted in the right rear flank of nu/nu mice. Lycorine treatment for 14 days significantly inhibited tumor growth compared with that in controls. Collectively, our findings suggest that lycorine suppressed the Akt pathway and activated the intrinsic apoptotic cascade, leading to the apoptosis of bladder cancer cells. We suggest that lycorine can be a viable therapeutic option for bladder cancer patients.
| Original language | English |
|---|---|
| Pages (from-to) | 197-202 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 483 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 29 2017 |
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Keywords
- Apoptosis
- Bladder cancer
- Lycorine
- Phospho-Akt
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Lycorine induces apoptosis of bladder cancer T24 cells by inhibiting phospho-Akt and activating the intrinsic apoptotic cascade. / Wang, Chongshan; Wang, Qiongren; Li, Xiezhao; Jin, Zhong; Xu, Peng; Xu, Naijin; Xu, Abai; Xu, Yawen; Zheng, Shaobo; Zheng, Junxia; Liu, Chunxiao; Huang, Peng.
In: Biochemical and Biophysical Research Communications, Vol. 483, No. 1, 29.01.2017, p. 197-202.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lycorine induces apoptosis of bladder cancer T24 cells by inhibiting phospho-Akt and activating the intrinsic apoptotic cascade
AU - Wang, Chongshan
AU - Wang, Qiongren
AU - Li, Xiezhao
AU - Jin, Zhong
AU - Xu, Peng
AU - Xu, Naijin
AU - Xu, Abai
AU - Xu, Yawen
AU - Zheng, Shaobo
AU - Zheng, Junxia
AU - Liu, Chunxiao
AU - Huang, Peng
PY - 2017/1/29
Y1 - 2017/1/29
N2 - Lycorine, an alkaloid extracted from Amaryllidaceae genera, exhibits antitumor activities against several human solid-tumor and leukemia cells with extensive influence on various cell signaling molecules. However, the effect of lycorine on bladder cancer has not yet been investigated. In this study, we demonstrated that lycorine induced apoptosis in human bladder cancer T24 cells, an effect that is mediated via inhibition of phospho-Akt expression and the consequent activation of caspase-3 and Bax in vitro. In an in vivo experiment, T24 cells were subcutaneously implanted in the right rear flank of nu/nu mice. Lycorine treatment for 14 days significantly inhibited tumor growth compared with that in controls. Collectively, our findings suggest that lycorine suppressed the Akt pathway and activated the intrinsic apoptotic cascade, leading to the apoptosis of bladder cancer cells. We suggest that lycorine can be a viable therapeutic option for bladder cancer patients.
AB - Lycorine, an alkaloid extracted from Amaryllidaceae genera, exhibits antitumor activities against several human solid-tumor and leukemia cells with extensive influence on various cell signaling molecules. However, the effect of lycorine on bladder cancer has not yet been investigated. In this study, we demonstrated that lycorine induced apoptosis in human bladder cancer T24 cells, an effect that is mediated via inhibition of phospho-Akt expression and the consequent activation of caspase-3 and Bax in vitro. In an in vivo experiment, T24 cells were subcutaneously implanted in the right rear flank of nu/nu mice. Lycorine treatment for 14 days significantly inhibited tumor growth compared with that in controls. Collectively, our findings suggest that lycorine suppressed the Akt pathway and activated the intrinsic apoptotic cascade, leading to the apoptosis of bladder cancer cells. We suggest that lycorine can be a viable therapeutic option for bladder cancer patients.
KW - Apoptosis
KW - Bladder cancer
KW - Lycorine
KW - Phospho-Akt
UR - http://www.scopus.com/inward/record.url?scp=85010441709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85010441709&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2016.12.168
DO - 10.1016/j.bbrc.2016.12.168
M3 - Article
C2 - 28042037
AN - SCOPUS:85010441709
VL - 483
SP - 197
EP - 202
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -