Lycorine induces apoptosis of bladder cancer T24 cells by inhibiting phospho-Akt and activating the intrinsic apoptotic cascade

Chongshan Wang, Qiongren Wang, Xiezhao Li, Zhong Jin, Peng Xu, Naijin Xu, Abai Xu, Yawen Xu, Shaobo Zheng, Junxia Zheng, Chunxiao Liu, Peng Huang

Research output: Contribution to journalArticle

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Abstract

Lycorine, an alkaloid extracted from Amaryllidaceae genera, exhibits antitumor activities against several human solid-tumor and leukemia cells with extensive influence on various cell signaling molecules. However, the effect of lycorine on bladder cancer has not yet been investigated. In this study, we demonstrated that lycorine induced apoptosis in human bladder cancer T24 cells, an effect that is mediated via inhibition of phospho-Akt expression and the consequent activation of caspase-3 and Bax in vitro. In an in vivo experiment, T24 cells were subcutaneously implanted in the right rear flank of nu/nu mice. Lycorine treatment for 14 days significantly inhibited tumor growth compared with that in controls. Collectively, our findings suggest that lycorine suppressed the Akt pathway and activated the intrinsic apoptotic cascade, leading to the apoptosis of bladder cancer cells. We suggest that lycorine can be a viable therapeutic option for bladder cancer patients.

Original languageEnglish
Pages (from-to)197-202
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume483
Issue number1
DOIs
Publication statusPublished - Jan 29 2017

Fingerprint

Urinary Bladder Neoplasms
Apoptosis
Tumors
Amaryllidaceae Alkaloids
Cell signaling
Alkaloids
Caspase 3
lycorine
Neoplasms
Leukemia
Chemical activation
Cells
Molecules
Therapeutics
Growth
Experiments

Keywords

  • Apoptosis
  • Bladder cancer
  • Lycorine
  • Phospho-Akt

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lycorine induces apoptosis of bladder cancer T24 cells by inhibiting phospho-Akt and activating the intrinsic apoptotic cascade. / Wang, Chongshan; Wang, Qiongren; Li, Xiezhao; Jin, Zhong; Xu, Peng; Xu, Naijin; Xu, Abai; Xu, Yawen; Zheng, Shaobo; Zheng, Junxia; Liu, Chunxiao; Huang, Peng.

In: Biochemical and Biophysical Research Communications, Vol. 483, No. 1, 29.01.2017, p. 197-202.

Research output: Contribution to journalArticle

Wang, Chongshan ; Wang, Qiongren ; Li, Xiezhao ; Jin, Zhong ; Xu, Peng ; Xu, Naijin ; Xu, Abai ; Xu, Yawen ; Zheng, Shaobo ; Zheng, Junxia ; Liu, Chunxiao ; Huang, Peng. / Lycorine induces apoptosis of bladder cancer T24 cells by inhibiting phospho-Akt and activating the intrinsic apoptotic cascade. In: Biochemical and Biophysical Research Communications. 2017 ; Vol. 483, No. 1. pp. 197-202.
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AU - Xu, Naijin

AU - Xu, Abai

AU - Xu, Yawen

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